Skip to main content
Erschienen in: Lasers in Medical Science 2/2013

01.02.2013 | Original Article

Effects of low-level laser therapy (LLLT) on bone repair in rats: optical densitometry analysis

verfasst von: Danillo Barbosa, Renato Aparecido de Souza, Murilo Xavier, Fabiano Fernandes da Silva, Emilia Ângela Loschiavo Arisawa, Antonio Guillermo Jose Balbin Villaverde

Erschienen in: Lasers in Medical Science | Ausgabe 2/2013

Einloggen, um Zugang zu erhalten

Abstract

The aim of this study was to evaluate the process of bone repair in rats submitted to low-level laser therapy using optical densitometry. A total of 45 rats which underwent femoral osteotomy were randomly distributed into three groups: control (group I) and laser-treated groups using wavelengths in the red (λ, 660–690 nm) and in the infrared (λ, 790–830 nm) spectra (group II and group III, respectively). The animals (five per group) were killed after 7, 14, and 21 days and the femurs were removed for optical densitometry analysis. Optical density showed a significant increase in the degree of mineralization (gray level) in both groups treated with the laser after 7 days. After 14 days, only the group treated with laser therapy in the infrared spectrum showed higher bone density. No differences were observed between groups after 21 days. Such results suggest the positive effect of low-level laser therapy in bone repair is time- and wavelength-dependent. In addition, our results have confirmed that optical densitometry technique can measure bone mineralization status.
Literatur
1.
Zurück zum Zitat Pinheiro ALB, Gerbi MEM, Limeira Júnior FA, Ponzi EAC, Marques AMC, Carvalho CM, Santos RC, Oliveira PC, Nóia M, Ramalho LMP (2009) Bone repair following bone grafting hydroxyapatite guided bone regeneration and infra-red laser photobiomodulation: a histological study in a rodent model. Lasers Med Sci 24:234–240. doi:10.1007/s10103-008-0556-0 PubMedCrossRef Pinheiro ALB, Gerbi MEM, Limeira Júnior FA, Ponzi EAC, Marques AMC, Carvalho CM, Santos RC, Oliveira PC, Nóia M, Ramalho LMP (2009) Bone repair following bone grafting hydroxyapatite guided bone regeneration and infra-red laser photobiomodulation: a histological study in a rodent model. Lasers Med Sci 24:234–240. doi:10.​1007/​s10103-008-0556-0 PubMedCrossRef
3.
Zurück zum Zitat Lopes CB, Pinheiro AL, Sathaiah S, Da Silva NS, Salgado MA (2007) Infrared laser photobiomodulation (lambda 830 nm) on bone tissue around dental implants: a Raman spectroscopy and scanning electronic microscopy study in rabbits. Photomed Laser Surg 25(2):96–101. doi:10.1089/pho.2006.2030 PubMedCrossRef Lopes CB, Pinheiro AL, Sathaiah S, Da Silva NS, Salgado MA (2007) Infrared laser photobiomodulation (lambda 830 nm) on bone tissue around dental implants: a Raman spectroscopy and scanning electronic microscopy study in rabbits. Photomed Laser Surg 25(2):96–101. doi:10.​1089/​pho.​2006.​2030 PubMedCrossRef
5.
Zurück zum Zitat Nicolau RA, Jorgetti V, Rigau J, Pacheco MT, Dos Reis LM, Zângaro RA (2003) Effect of low-power GaAlAs laser (660 nm) on bone structure and cell activity: an experimental animal study. Lasers Med Sci 18:89–94. doi:10.1007/s10103-003-0260-z CrossRef Nicolau RA, Jorgetti V, Rigau J, Pacheco MT, Dos Reis LM, Zângaro RA (2003) Effect of low-power GaAlAs laser (660 nm) on bone structure and cell activity: an experimental animal study. Lasers Med Sci 18:89–94. doi:10.​1007/​s10103-003-0260-z CrossRef
6.
Zurück zum Zitat Karu T (1987) Phobobiological fundamental of low power laser therapy. IEEE J Quantum Electron 23:1703CrossRef Karu T (1987) Phobobiological fundamental of low power laser therapy. IEEE J Quantum Electron 23:1703CrossRef
7.
Zurück zum Zitat Schindl A, Schindl M, Pernerstorfer-Schön H, Schindl L (2000) Low-intensity laser therapy: a review. J Investig Med 48(5):312–326PubMed Schindl A, Schindl M, Pernerstorfer-Schön H, Schindl L (2000) Low-intensity laser therapy: a review. J Investig Med 48(5):312–326PubMed
8.
Zurück zum Zitat Nascimento SB, Cardoso CA, Ribeiro TP, Almeida JD, Albertini R, Munin E, Arisawa EA (2010) Effect of low-level laser therapy and calcitonin on bone repair in castrated rats: a densitometric study. Photomed Laser Surg 28(1):45–49. doi:10.1089/pho.2008.2396 PubMedCrossRef Nascimento SB, Cardoso CA, Ribeiro TP, Almeida JD, Albertini R, Munin E, Arisawa EA (2010) Effect of low-level laser therapy and calcitonin on bone repair in castrated rats: a densitometric study. Photomed Laser Surg 28(1):45–49. doi:10.​1089/​pho.​2008.​2396 PubMedCrossRef
9.
Zurück zum Zitat Da Silva RV, Camilli JA (2006) Repair of bone defects treated with autogenous bone graft and low-power laser. J Craniofac Surg 17:297–301PubMedCrossRef Da Silva RV, Camilli JA (2006) Repair of bone defects treated with autogenous bone graft and low-power laser. J Craniofac Surg 17:297–301PubMedCrossRef
10.
Zurück zum Zitat Freitas IGF, Baranauskas V, Cruz-Höfling MA (2000) Laser effects on osteogenesis. Appl Surf Sci 154–155:548–554CrossRef Freitas IGF, Baranauskas V, Cruz-Höfling MA (2000) Laser effects on osteogenesis. Appl Surf Sci 154–155:548–554CrossRef
12.
Zurück zum Zitat Trelles MA, Mayayo E (1987) Bone fracture consolidates faster with low-power laser. Lasers Surg Med 7(1):36–45PubMedCrossRef Trelles MA, Mayayo E (1987) Bone fracture consolidates faster with low-power laser. Lasers Surg Med 7(1):36–45PubMedCrossRef
13.
Zurück zum Zitat Garavello-Freitas I, Baranauskas V, Joazeiro P, Padovani CR, Dal Pai-Silva M, Da Cruz-Hofling MA (2003) Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J Photochem B Photobiol 70:81–89. doi:10.1016/S1011-1344(03)00058-7 CrossRef Garavello-Freitas I, Baranauskas V, Joazeiro P, Padovani CR, Dal Pai-Silva M, Da Cruz-Hofling MA (2003) Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J Photochem B Photobiol 70:81–89. doi:10.​1016/​S1011-1344(03)00058-7 CrossRef
16.
Zurück zum Zitat Anneroth G, Hall G, Rydén H, Zetterqvist L (1988) The effect of low-energy infrared laser radiation on wound healing in rats. Br J Oral Maxillofac Surg 26:12–17PubMedCrossRef Anneroth G, Hall G, Rydén H, Zetterqvist L (1988) The effect of low-energy infrared laser radiation on wound healing in rats. Br J Oral Maxillofac Surg 26:12–17PubMedCrossRef
18.
Zurück zum Zitat Vladimirov YA, Osipov AN, Klebanov GI (2004) Photobiological principles of therapeutic applications of laser radiation. Biochemistry 69(1):81–90PubMed Vladimirov YA, Osipov AN, Klebanov GI (2004) Photobiological principles of therapeutic applications of laser radiation. Biochemistry 69(1):81–90PubMed
19.
Zurück zum Zitat Martínez MEM, Pinheiro ALB, Ramalho LMP (2008) Effect of IR laser photobiomodulation on the repair of bone defects grafted with organic bovine bone. Lasers Med Sci 23:313–317. doi:10.1007/s10103-007-0488-0 CrossRef Martínez MEM, Pinheiro ALB, Ramalho LMP (2008) Effect of IR laser photobiomodulation on the repair of bone defects grafted with organic bovine bone. Lasers Med Sci 23:313–317. doi:10.​1007/​s10103-007-0488-0 CrossRef
20.
Zurück zum Zitat Queiroga AS, Sousa FB, Araújo JMS, Santos SD, Sousa CD’FS, Quintans TC, Almeida TP, Nonaka CFW, Batista LV, Limeira Junior FA (2008) Evaluation of bone repair in the femur of rats submitted to laser therapy in different wavelengths: an image segmentation method of analysis. Laser Phys 18(9):1087–1091. doi:10.1134/S1054660X08090156 CrossRef Queiroga AS, Sousa FB, Araújo JMS, Santos SD, Sousa CD’FS, Quintans TC, Almeida TP, Nonaka CFW, Batista LV, Limeira Junior FA (2008) Evaluation of bone repair in the femur of rats submitted to laser therapy in different wavelengths: an image segmentation method of analysis. Laser Phys 18(9):1087–1091. doi:10.​1134/​S1054660X0809015​6 CrossRef
23.
Zurück zum Zitat Taba M, Novaes AB, Souza SLS, Grisi MFM, Palioto DB, Pardini LC (2003) Radiographic evaluation of dental implants with different surface treatments: an experimental study in dogs. Implant Dent 12(3):252–258CrossRef Taba M, Novaes AB, Souza SLS, Grisi MFM, Palioto DB, Pardini LC (2003) Radiographic evaluation of dental implants with different surface treatments: an experimental study in dogs. Implant Dent 12(3):252–258CrossRef
24.
Zurück zum Zitat Martins MV, Da Silva MA, Medici Filho E, De Moraes LC, Castilho JC, Da Rocha RF (2005) Evaluation of digital optical density of bone repair in rats medicated with ketoprofen. Braz Dent J 16:207–212PubMedCrossRef Martins MV, Da Silva MA, Medici Filho E, De Moraes LC, Castilho JC, Da Rocha RF (2005) Evaluation of digital optical density of bone repair in rats medicated with ketoprofen. Braz Dent J 16:207–212PubMedCrossRef
25.
Zurück zum Zitat Sakakura CE, Giro G, Gonçalves D, Pereira RM, Orrico SR, Marcantonio E Jr (2006) Radiographic assessment of bone density around integrated titanium implants after ovariectomy in rats. Clin Oral Implants Res 17:134–138PubMedCrossRef Sakakura CE, Giro G, Gonçalves D, Pereira RM, Orrico SR, Marcantonio E Jr (2006) Radiographic assessment of bone density around integrated titanium implants after ovariectomy in rats. Clin Oral Implants Res 17:134–138PubMedCrossRef
26.
Zurück zum Zitat Gerbi MEM, Pinheiro ALB, Marzola C, Limeira Júnior FA, Ramalho LMP, Ponzi EAC, Soares AO, Carvalho LCB, Lima HV, Gonçalves TO (2005) Assessment of bone repair associated with the use of organic bovine bone and membrane irradiated at 830 nm. Photomed Laser Surg 23(4):382–388. doi:10.1089/pho.2005.23.382 PubMedCrossRef Gerbi MEM, Pinheiro ALB, Marzola C, Limeira Júnior FA, Ramalho LMP, Ponzi EAC, Soares AO, Carvalho LCB, Lima HV, Gonçalves TO (2005) Assessment of bone repair associated with the use of organic bovine bone and membrane irradiated at 830 nm. Photomed Laser Surg 23(4):382–388. doi:10.​1089/​pho.​2005.​23.​382 PubMedCrossRef
30.
Zurück zum Zitat Javadieh F, Bayat M, Abdi S, Mohsenifar Z, Razi S (2009) The effects of infrared low-level laser therapy on healing of partial osteotomy of tibia in streptozotocin-induced diabetic rats. Photomed Laser Surg 27(4):641–646. doi:10.1089/pho.2008.2370 PubMedCrossRef Javadieh F, Bayat M, Abdi S, Mohsenifar Z, Razi S (2009) The effects of infrared low-level laser therapy on healing of partial osteotomy of tibia in streptozotocin-induced diabetic rats. Photomed Laser Surg 27(4):641–646. doi:10.​1089/​pho.​2008.​2370 PubMedCrossRef
Metadaten
Titel
Effects of low-level laser therapy (LLLT) on bone repair in rats: optical densitometry analysis
verfasst von
Danillo Barbosa
Renato Aparecido de Souza
Murilo Xavier
Fabiano Fernandes da Silva
Emilia Ângela Loschiavo Arisawa
Antonio Guillermo Jose Balbin Villaverde
Publikationsdatum
01.02.2013
Verlag
Springer-Verlag
Erschienen in
Lasers in Medical Science / Ausgabe 2/2013
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-012-1125-0

Weitere Artikel der Ausgabe 2/2013

Lasers in Medical Science 2/2013 Zur Ausgabe