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01.09.2013 | Original Article

Efficacy of quantitative RT-PCR for detection of the nucleoprotein gene from different porcine rubulavirus strains

verfasst von: José Francisco Rivera-Benitez, Adelfa del Carmen García-Contreras, Julio Reyes-Leyva, Jesús Hernández, José Iván Sánchez-Betancourt, Humberto Ramírez-Mendoza

Erschienen in: Archives of Virology | Ausgabe 9/2013

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Abstract

Blue-eye disease is an emergent viral swine infection caused by porcine rubulavirus (PoRV). We have developed a qRT-PCR method to detect and quantify expression of the nucleoprotein gene for different PoRV strains. The limit of detection for this assay was 10² copies of synthetic RNA. Viral RNA from PoRV was detectable at a TCID₅₀ of 0.01. Significant differences were observed between viral RNA quantification and virus titration results for nine PoRV strains. For nasal and oral swab samples that were collected from experimentally infected pigs, the qRT-PCR assay was more sensitive (87.1–83.9 %) for the detection of positive samples than methods involving isolation of virus. The implementation of highly sensitive assays that yield results quickly will be of great assistance in the eradication of PoRV from Mexico. We also believe that the newly developed qRT-PCR assay will help reduce the spread of this viral infection to other countries.

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Titel: Efficacy of quantitative RT-PCR for detection of the nucleoprotein gene from different porcine rubulavirus strains
verfasst von: José Francisco Rivera-Benitez
Adelfa del Carmen García-Contreras
Julio Reyes-Leyva
Jesús Hernández
José Iván Sánchez-Betancourt
Humberto Ramírez-Mendoza
Publikationsdatum: 01.09.2013
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 9/2013
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-013-1672-0

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