Background
Methods
Cell lines from cell banks
Cell isolation and establishment of cell lines
Transmission electron microscopy (TEM) examination
Evaluation of culture purity and cell cycle status by flow cytometry
Cell Counting Kit (CCK)-8 cell growth assay
TP53 mutation screening
Target | Forward primer (5′→3′) | Reverse primer (5′→3′) | Product (bp) | Codon position |
---|---|---|---|---|
Exon 1 | GTCGGCGAGAATCCTGACT | CCAACAATGCAACTCCTATGAT | 481 | 0 |
Exon 2 | TCAGACACTGGCATGGTGTT | GGGGACAGCATCAAATCATC | 498 | 1–25 |
Exon 3 | TCAGACACTGGCATGGTGTT | GGGGACAGCATCAAATCATC | 498 | 25–32 |
Exon 4 | GGGACTGACTTTCTGCTCTTGT | GCCAAAGGGTGAAGAGGAATC | 543 | 33–125 |
Exon 5 | GTTTCTTTGCTGCCGTCTTC | TTCCTTCCACTCGGATAAGATG | 387 | 126–187 |
Exon 6 | TACAAGCAGTCACAGCACATGA | GGTCAAATAAGCAGCAGGTTAAGA | 370 | 187–224 |
Exon 7 | GTGAAACCCCGTCTCTACTGAA | GAGGAGAAGCCACAGGTTAAGA | 588 | 225–261 |
Exon 8 | GGAGTAGATGGAGCCTGGTTTT | GTTGGGCAGTGCTAGGAAAG | 328 | 261–307 |
Exon 9 | GGTAAGCAAGCAGGACAAGAAG | TACAACCAGGAGCCATTGTCTT | 313 | 307–331 |
Exon 10 | CATGTTGCTTTTGTACCGTCAT | TGGATACACTGAGGCAAGAATG | 408 | 332–367 |
Exon 11 | AACATATTTGCATGGGGTGTG | CCAGTCTCCAGCCTTTGTTC | 1580 | 367–394 |