Erschienen in:
01.10.2001 | Commentary
Ex vivo gene transfer in the years to come
verfasst von:
Thomas Pap, Renate E Gay, Ulf Müller-Ladner, Steffen Gay
Erschienen in:
Arthritis Research & Therapy
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Ausgabe 1/2001
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Abstract
Synovial fibroblasts (SFs) have become a major target for ex vivo gene transfer in rheumatoid arthritis (RA), but efficient transduction of RA-SFs still is a major problem. The low proliferation rate and heterogeneity of RA-SFs, together with their lack of highly specific surface receptors, have hampered a more extensive application of this technique. Improving transduction protocols with conventional viral vectors, therefore, as well as developing novel strategies, such as alternative target cells, and novel delivery systems constitute a major challenge. Recent progress in this field will lead to the achievement of high transgene expression, and will facilitate the use of gene transfer in human trials.