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Erschienen in:
08.08.2017 | Gynecologic Endocrinology and Reproductive Medicine
Expression of H19 imprinted gene in patients with repeated implantation failure during the window of implantation
Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 4/2017
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Purpose
To detect the expression level of imprinted gene H19 in the endometrium of patients with repeated implantation failure (RIF) who were undergoing in vitro fertilization and embryo transfer (IVF-ET) or frozen embryo transfer (FET).
Methods
Patients who underwent IVF/FET in Reproductive Medicine Center of Xiangya Hospital during April 1, 2015 to October 31, 2015 were chosen. The endometrium was collected from patients with repeated implantation failure during mid-luteal phase. Real-time quantitative reverse transcription PCR (qRT-PCR) and western blot were used to determine the expression of H19 lncRNA and integrin β3 protein. Data analysis was performed using SPSS 19.0.
Results
When compared with the control group, decreased expression of H19 was detected in the RIF group (P = 0.04). The expression of integrin β3 protein was also decreased in the RIF group compared to that in the control group (P = 0.04). The expression of H19 lncRNA was positively correlated with the expression of integrin β3 protein (R = 0.477, P = 0.018).
Conclusions
The expression of H19 lncRNA and integrin β3 protein were down-regulated in the RIF patients.