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Archives of Gynecology and Obstetrics
Erschienen in:

08.08.2017 | Gynecologic Endocrinology and Reproductive Medicine

Expression of H19 imprinted gene in patients with repeated implantation failure during the window of implantation

verfasst von: Hong Zeng, Xiuli Fan, Nenghui Liu

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 4/2017

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Abstract

Purpose

To detect the expression level of imprinted gene H19 in the endometrium of patients with repeated implantation failure (RIF) who were undergoing in vitro fertilization and embryo transfer (IVF-ET) or frozen embryo transfer (FET).

Methods

Patients who underwent IVF/FET in Reproductive Medicine Center of Xiangya Hospital during April 1, 2015 to October 31, 2015 were chosen. The endometrium was collected from patients with repeated implantation failure during mid-luteal phase. Real-time quantitative reverse transcription PCR (qRT-PCR) and western blot were used to determine the expression of H19 lncRNA and integrin β3 protein. Data analysis was performed using SPSS 19.0.

Results

When compared with the control group, decreased expression of H19 was detected in the RIF group (P = 0.04). The expression of integrin β3 protein was also decreased in the RIF group compared to that in the control group (P = 0.04). The expression of H19 lncRNA was positively correlated with the expression of integrin β3 protein (R = 0.477, P = 0.018).

Conclusions

The expression of H19 lncRNA and integrin β3 protein were down-regulated in the RIF patients.
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Metadaten
Titel
Expression of H19 imprinted gene in patients with repeated implantation failure during the window of implantation
verfasst von
Hong Zeng
Xiuli Fan
Nenghui Liu
Publikationsdatum
08.08.2017
Verlag
Springer Berlin Heidelberg
Erschienen in
Archives of Gynecology and Obstetrics / Ausgabe 4/2017
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI
https://doi.org/10.1007/s00404-017-4482-x

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