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Journal of NeuroVirology

Erschienen in:

09.07.2018

Expression of pseudorabies virus-encoded long noncoding RNAs in epithelial cells and neurons

verfasst von: Xiang Guan, Jie Liu, Hui Jiang, Chang-Xian Wu, Huan-Chun Chen, Zheng-Fei Liu

Erschienen in: Journal of NeuroVirology | Ausgabe 5/2018

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Abstract

Long noncoding RNAs (lncRNAs) play important roles in regulating eukaryotic genome replication and gene expression in diverse biological systems. Here, we identified lncRNAs transcribed from pseudorabies virus (PRV)-infected PK-15 cells. Based on high-throughput sequencing data, we obtained 87,263,926 and 93,947,628 clean reads from mock-infected and PRV-infected PK-15 cells, respectively. Through a normalized analytic protocol, we identified three novel viral lncRNAs. According to an analysis of differential expression between the mock-infected and PRV-infected cells, 4151 host lncRNAs were significantly upregulated and 2327 host lncRNAs were significantly downregulated in the latter group. Viral lncRNAs and several host lncRNAs were verified by northern blotting and real-time PCR. The findings showed that the viral lncRNA LDI might regulate the expression of IE180, a potent transcriptional activator of viral genes. Furthermore, we characterized the expression of viral lncRNAs in a culture of infected primary chicken dorsal root ganglia (DRG). Collectively, the obtained data suggest that PRV generates lncRNAs in both epithelial cells and chick DRG neurons.

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Titel: Expression of pseudorabies virus-encoded long noncoding RNAs in epithelial cells and neurons
verfasst von: Xiang Guan
Jie Liu
Hui Jiang
Chang-Xian Wu
Huan-Chun Chen
Zheng-Fei Liu
Publikationsdatum: 09.07.2018
Verlag: Springer International Publishing
Erschienen in: Journal of NeuroVirology / Ausgabe 5/2018
Print ISSN: 1355-0284
Elektronische ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-018-0651-3

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