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Calcified Tissue International

Erschienen in:

01.10.2003 | Laboratory Investigations

FGF-2 Regulates Enamel and Dentine Formation in Mouse Tooth Germ

verfasst von: T. Tsuboi, S. Mizutani, M. Nakano, K. Hirukawa, A. Togari

Erschienen in: Calcified Tissue International | Ausgabe 5/2003

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Abstract

We examined the effects of basic fibroblast growth factor (FGF-2) on cultured lower molar tooth germ at the differentiative (bell) stage. Although FGF-2 has been detected in odontogenesis, its roles in biological activities, such as cell proliferation, differentiation and extracellular matrix mineralization are unclear. We assayed mRNA levels of the differentiation markers, dentine sialophosphoprotein (DSPP), amelogenin and alkaline phosphatase (ALP) using reverse transcription-polymerase chain reaction (RT-PCR), and histological methods. Tooth germs dissected from 17-day-old embryonic mice were cultured for 4 days with either recombinant human FGF-2 or specific antisense phosphorothioate oligodeoxynucleotide (antisense ODN) for FGF-2. Exogenous FGF-2 decreased the gene expression of differentiation markers in molars at the bell stage. Abrogation of endogenous FGF-2 by antisense ODN increased the gene expression of differentiation markers, and also significantly enhanced enamel and dentine formation. This histological change was recovered by adding exogeneous FGF-2. These findings suggest that FGF-2 at the bell stage regulates cell differentiation and matrix secretion.

Klagsbrun, M 1989The fibroblast growth factor: structural and biological properties.Prog Growth Fact Res1207235

Gospodarowitz, D, Ferrara, N 1987Structural characterization and biological functions of fibroblast growth factor.Endocr Rev895114PubMed

Cam, Y, Neumann, MR, Oliver, L, Raulais, D, Janet, T, Ruch, JV 1992Immunolocalization of acidic and basic fibroblast growth factors during mouse odontogenesis.Int J Dev Biol36381389PubMed

Kettunen, P, Karavanova, I, Thesleff, I 1998Responsiveness of developing dental tissues to fibroblast growth factors: expression of splicing alternatives of FGFR1, -2, -3, and of FGFR4 and stimulation of cell proliferation by FGF-2, -4, -8, and -9.Dev Genet22374385PubMed

D’Souza, RN, Cavender, A, Sunavala, G, Alvarez, J, Ohshima, T, Kulkarni, AB, Macdougall, M 1997Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo.J Bone Miner Res1220402049PubMed

Bleicher, F, Couble, ML, Farges, JC, Couble, P, Magloire, H 1999Sequential expression of matrix protein genes in developing rat teeth.Matrix Biol18133143CrossRefPubMed

Oida, S, Nagano, T, Yamakoshi, Y, Ando, H, Yamada, M, Fukae, M 2002Amelogenin gene expression in porcine odontoblasts.J Dent Res81103108PubMed

Papagerakis, P, Berdal, A, Mesbah, M, Peuchmaur, M, Malaval, L, Nydegger, J, Simmer, J, Macdougall, M 2002Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth.Bone30377385CrossRefPubMed

Sasaki, S, Shimokawa, H 1995The amelogenin gene.Int J Dev Biol39127133PubMed

10.

Robey, PG 1996Vertebrate mineralized matrix proteins: structure and function.Connect Tissue Res35131136

11.

Hu, CC, Sakakura, Y, Sasano, Y, Shum, L, Bringas, P, Werb, Z, Slavkin, HC 1992Endogenous epidermal growth factor regulates the timing and pattern of embryonic mouse molar tooth morphogenesis.Int J Dev Biol36505516PubMed

12.

Slavkin, HC 1995Antisense oligonucleotides: an experimental strategy to advance a causal analysis of development.Int J Dev Biol39123126PubMed

13.

Tsuboi, T, Togari, A 1996Comparison of the effects of carboxyl-terminal parathyroid hormone peptide [53-84] and aminoterminal peptide [1-34] on mouse tooth germ in vitro.Arch Oral Biol35131136

14.

Hebert, JM, Basilico, C, Goldfarb, M, Haub, O, Martin, GR 1990Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis.Dev Biol138454463PubMed

15.

MacDougall, M, Simmons, D, Luan, X, Nydegger, J, Feng, J, Gu, TT 1997Dentin phosphoprotein and dentin sialoprotein are cleavage products expressed from a single transcript coded by a gene on human chromosome 4, dentine phosphoprotein DNA sequence determination.J Biol Chem272835842CrossRefPubMed

16.

Snead, ML, Lau, EC, Zeichner-David, M, Fincham, AG, Woo, SLC, Slavkin, HC 1985DNA sequence for cloned cDNA for murine amelogenin reveal the amino acid sequence for enamel-specific protein.Biochem Biophys Res Commun129812818PubMed

17.

Weiss, MJ, Cole, DE, Ray, K, Whyte, MP, Lafferty, MA, Mulivor, RA, Harris, H 1988A missense mutation in the human liver/bone/kidney alkaline phosphatase gene causing a lethal form of hypophosphatasia.Proc Natl Acad Sci USA8576667669PubMed

18.

Arcari, P, Martinelli, R, Salvatore, F 1984The complete sequence of a full length cDNA for human liver glyceraldehyde-3-phosphate dehydrogenase: evidence for multiple mRNA species.Nucleic Acids Res1291799189PubMed

19.

Mansukhani, A, Moscatelli, D, Talarico, D, Levytska, V, Basilico, C 1990A murine flbroblast growth factor (FGF) receptor expressed in CHO cells is activated by basic FGF kaposi FGF.Proc Natl Acad Sci USA8743784382PubMed

20.

Mansukhani, A, Dell’Era, P, Moscatelli, D, Kornbluth, S, Hanafusa, H, Basilico, C 1992Characterization of the murine BEK flbroblast growth factor (FGF) receptor: activation by three members of the FGF family and requirement for heparin.Proc Natl Acad Sci USA8933053308PubMed

21.

Heid, CA, Stevens, J, Livak, KJ, Williams, PM 1996Real time quantitative PCR.Genome Res6986994PubMed

22.

Russo, LG, Maharajan, P, Maharajan, V 1998Basic fibroblast growth factor (FGF-2) in mouse tooth morphogenesis.Growth factor15125133PubMed

23.

Ornitz, DM, Xu, J, Colvin, JS, McEwen, DG, MacArthur, CA, Coulier, F, Gao, G, Goldfarb, M 1996Receptor specificity of the fibroblast growth factor family.J Biol Chem2711529215297PubMed

24.

Nakamura, T, Hara, Y, Tagawa, M, Tamura, M, Yuge, T, Fukuda, H, Nigi, H 1998Recombinant human basic fibroblast growth factor accelerates fracture healing by enhancing callus remodeling in experimental dog tibial fracture.J Bone Miner Res13942949PubMed

25.

Takayama, S, Murakami, S, Shimabukuro, Y, Kitamura, M, Okada, H 2001Periodontal regeneration by FGF-2 (bFGF) in primate models.J Dent Res8020752079PubMed

26.

Canalis, E, Centrella, M, McCarthy, T 1988Effects of basic fibroblast growth factor on bone formation in vitro.J Clin Invest8115721577PubMed

27.

Rodan, SB, Wesolowski, G, Yoon, K, Rodan, GA 1989Opposing effects of fibroblast growth factor and pertussis toxin on alkaline phosphatase, osteopontin, osteocalcin, and type 1 collagen mRNA levels in ROS 17/2.8 cells.J Biochem2641993419941

28.

Debiais, F, Hott, M, Graulet, AM, Marie, PJ 1998The effects of fibroblast growth factor-2 on human neonatal calvaria osteoblastic cells are differentiation stage specific.J Bone Miner Res13645654PubMed

29.

Yamamoto, T, Katayama, I, Nishioka, K 1998Inhibitory effects of basic fibroblast growth factor antisense oligodeoxynucleotide on in vitro proliferation of mouse 3T3 fibroblasts.Arch Dermatol Res290171173CrossRefPubMed

30.

Varghese, S, Ramsby, ML, Jeffrey, JJ, Canalis, E 1995Basic fibroblast growth factor stimulates expression of interstitial collagenase and metalloproteinases in rat bone cells.Endocrinology13621562162PubMed

31.

Kato, Y, Iwamoto, M 1990Fibroblast growth factor is an inhibitor of chondorocyte terminal differentiation.J Biol Chem26559035909PubMed

32.

Wroblewski, J, Edwall-Arvidsson, C 1995Inhibitory effects of basic fibroblast growth factor on chondrocyte differentiation.J Bone Miner Res10735742PubMed

33.

Couble, ML, Farges, JC, Bleicher, F, Perrat-Mabillon, B, Boudeulle, M, Magloire, H 2000Odontoblast differentiation of human dental pulp cells in explant cultures.Calcif Tissue Int66129138PubMed

34.

Shiba, H, Nakamura, S, Shirakawa, M, Nakanishi, K, Okamoto, H, Satakeda, H, Noshiro, M, Kamihagi, K, Katayama, M, Kato, Y 1995Effects of basic fibroblast growth factor on proliferation, the expression of osteonectin (SPARC) and alkaline phosphatase, and calcification in cultures of human pulp cells.Dev Biol170457466PubMed

35.

Unda, FJ, Martin, A, Hilario, E, Begue-Kirn, C, Ruch, JV, Arechaga, J 2000Dissection of the odontoblast differentiation process in vitro by a combination of FGF1, FGF2, and TGFβ1.Dev Dyn218480489CrossRefPubMed

36.

Zhou, M, Sutliff, RL, Paul, RJ, et al. 1998Fibroblast growth factor 2 control of vascular tone.Nat Med4201207PubMed

37.

Coffin, JD, Florkiewicz, RZ, Neumann, J, Mort-Hopkins, T, Dorn II, GW, Lightfoot, P, German, R, Howles, PN, Kier, A, O’Toole, BA 1995Abnormal bone growth and selective translational regulation in basic fibroblast growth factor (FGF-2) transgenic mice.Mol Biol Cell618611873PubMed

Titel: FGF-2 Regulates Enamel and Dentine Formation in Mouse Tooth Germ
verfasst von: T. Tsuboi
S. Mizutani
M. Nakano
K. Hirukawa
A. Togari
Publikationsdatum: 01.10.2003
Verlag: Springer-Verlag
Erschienen in: Calcified Tissue International / Ausgabe 5/2003
Print ISSN: 0171-967X
Elektronische ISSN: 1432-0827
DOI: https://doi.org/10.1007/s00223-002-4070-2

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