nach oben

Erschienen in:

25.11.2016 | Original Article

Final irrigation protocols may affect intraradicular dentin ultrastructure

verfasst von: Márcia Helena Wagner, Ricardo Abreu da Rosa, José Antonio Poli de Figueiredo, Marco Antonio Húngaro Duarte, Jefferson Ricardo Pereira, Marcus Vinicius Reis Só

Erschienen in: Clinical Oral Investigations | Ausgabe 7/2017

Einloggen, um Zugang zu erhalten

Abstract

Objectives

The aim of this study was to evaluate the effect of different irrigation protocols on the root dentin structure using scanning electron microscopy (SEM) and transmission electron microscopy (TEM).

Materials and methods

Thirty-nine lower bovine incisors were hemisected longitudinally and randomly divided into 13 groups (n = 3). After the root halves were reassembled, it was applied a specific irrigation protocol for each group, as following: G1, distilled water (control); G2, 0.9% saline; G3, saline + 17% EDTA; G4, saline + PUI; G5, saline + PUI + EDTA; G6 to G9 received the same protocol as above replacing 0.9% saline by 2.5% NaOCl; and G10 to G13 by 2% CHX. One-half of each sample was prepared and evaluated using SEM and the other one by TEM observations.

Results

TEM descriptive analysis showed modifications in dentin organic ultrastructure, characterized by the thinning of dentin collagen fibrils, caused by NaOCl, enhanced by EDTA and/or PUI. SEM analysis showed that NaOCl with PUI caused significantly larger erosion of the peritubular dentin than in all the other groups (P < 0.05), followed by NaOCl + EDTA and NaOCl + EDTA + PUI.

Conclusions

NaOCl caused ultrastructural alterations in the dentin collagen, and enhanced by EDTA and/or PUI, promoted peritubular and intertubular erosion.

Clinical relevance

The effect of irrigating solutions on dentin ultrastructure was still unclear. The acknowledgment about the kind of solution, concentrations, application time, and sequence of use was important to achieve the right sanitization without jeopardizing the dentin ultrastructure quality.

Nair PNR (2006) On the causes of persistent apical periodontitis: a review. Int Endod J 39:249–281CrossRefPubMed

Zehnder M (2006) Root canal irrigants. J Endod 32:389–398CrossRefPubMed

Haapasalo M, Shen Y, Qian W, Gao Y (2010) Irrigation in endodontics. Dent Clin N Am 54:291–312CrossRefPubMed

Rôças IN, Siqueira JF (2011) Comparison of the in vivo antimicrobial effectiveness of sodium hypochlorite and chlorhexidine used as root canal irrigants: a molecular microbiology study. J Endod 37:143–150CrossRefPubMed

Clark-Holke D, Drake D, Walton R, Rivera E, Guthmiller JM (2003) Bacterial penetration through canals of endodontically treated teeth in the presence or absence of the smear layer. J Dent 31:275–281CrossRefPubMed

van der Sluis LWM, Versluis M, Wu MK, Wesselink PR (2007) Passive ultrasonic irrigation of the root canal: a review of the literature. Int Endod J 40:415–426CrossRefPubMed

van der Sluis LWM, Vogels MPMJ, Verhaagen B, Macedo R, Wesselink PR (2010) Study on influence of refreshment/activation cycles and irrigants on mechanical cleaning efficiency during ultrasonic activation of the irrigant. J Endod 36:737–740CrossRefPubMed

Gregorio C, Estevez R, Cisneros R, Paranjpe A, Cohenca N (2010) Efficacy of different irrigation and activation systems on the penetration of sodium hypochlorite into simulated lateral canals and up to working length: an in vitro study. J Endod 36:1216–1221CrossRefPubMed

Vera J, Arias A, Romero M (2011) Effect of maintaining apical patency on irrigant penetration into the apical third of root canals when using passive ultrasonic irrigation. J Endod 37:1276–1278CrossRefPubMed

10.

Torabinejad M, Cho Y, Khademi AA, Bakland LK, Shabahang S (2003) The effect of various concentrations of sodium hypochlorite on the ability of MTAD to remove the smear layer. J Endod 29:233–239CrossRefPubMed

11.

Arslan H, Ayranci LB, Karatas E, Topçuoğlu HS, Yavuz MS, Kesim B (2013) Effect of agitation of EDTA with 808-nanometer diode laser on removal of smear layer. J Endod 39:1589–1592CrossRefPubMed

12.

Ferraz CCR, Gomes BPFA, Zaia AA, Teixeira FB, Souza-Filho FJ (2001) In vitro assessment of the antimicrobial action and the mechanical ability of chlorhexidine gel as an endodontic irrigant. J Endod 27:452–455CrossRefPubMed

13.

Santos JN, Carrilho MRO, De Goes MF, Zaia AA, Gomes BPFA, Souza-Filho FJ, Ferraz CCR (2006) Effect of chemical irrigants on the bond strength of a self-etching adhesive to pulp chamber dentin. J Endod 32:1088–1090CrossRefPubMed

14.

Baumgartner JC, Mader CL (1987) A scanning electron microscopic evaluation of four root canal irrigation regimens. J Endod 13:147–156CrossRefPubMed

15.

Oyarzún A, Cordero AM, Whittle M (2002) Immunohistochemical evaluation of the effects of sodium hypochlorite on dentin collagen and glycosaminoglycans. J Endod 28:152–156CrossRefPubMed

16.

John C, Löst C, Elayouti A (2013) Ultrasonic monitoring of the effect of sodium hypochlorite on the elasticity of dentine. Int Endod J 46:477–482CrossRefPubMed

17.

Kuah HG, Lui JN, Tseng PSK, Chen NN (2009) The effect of EDTA with and without ultrasonics on removal of the smear layer. J Endod 35:393–396CrossRefPubMed

18.

Jiang LM, Verhaagen B, Versluis M, Zangrillo C, Cuckovic D, van der Sluis LWM (2010) An evaluation of the effect of pulsed ultrasound on the cleaning efficacy of passive ultrasonic irrigation. J Endod 36:1887–1891CrossRefPubMed

19.

Çalt S, Serper A (2002) Time-dependent effects of EDTA on dentin structures. J Endod 28:17–19CrossRefPubMed

20.

Şen BH, Ertürk O, Pişkin B (2009) The effect of different concentrations of EDTA on instrumented root canals walls. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 108:622–627CrossRefPubMed

21.

Mai S, Kim YK, Arola DD, Gu L, Kim JR, Pashley DH, Tay FR (2010) Differential aggressiveness of ethylenediamine tetraacetic acid in causing canal wall erosion in the presence of sodium hypochlorite. J Dent 38:201–206CrossRefPubMed

22.

Zhang K, Tay FR, Kim YK, Mitchell JK, Kim JR, Carrilho M, Pashley DH, Ling J (2010) The effect of initial irrigation with two different sodium hypochlorite concentrations on the erosion of instrumented radicular dentin. Dent Mater 26:514–523CrossRefPubMed

23.

Qian W, Shen Y, Haapasalo M (2011) Quantitative analysis of the effect of irrigant solution sequences on dentin erosion. J Endod 37:1437–1441CrossRefPubMed

24.

Uzunoglu E, Aktemur S, Uyanik MO, Durmaz V, Nagas E (2012) Effect of ethylenediaminetetraacetic acid on root fracture with respect to concentration at different time exposures. J Endod 38:1110–1113CrossRefPubMed

25.

Morris MD, Lee KW, Agee KA, Bouillaguet S, Pashley DH (2001) Effects of sodium hypochlorite and RC-prep on bond strengths of resin cement to endodontic surfaces. J Endod 27:753–757CrossRefPubMed

26.

Ari H, Yaşar E, Bellí S (2003) Effects of NaOCl on bond strengths of resin cements to root canal dentin. J Endod 29:248–251CrossRefPubMed

27.

Marending M, Luder HU, Brunner TJ, Knecht S, Stark WJ, Zehnder M (2007) Effects of sodium hypochlorite on human root dentine-mechanical, chemical and structural evaluation. Int Endod J 40:786–793CrossRefPubMed

28.

Pereira JR, Valle AL, Ghizoni JS, Lorenzoni FC, Ramos MB, Só MVR (2013) Push-out bond strengths of different dental cements used to cement glass fiber posts. J Prosthet Dent 110:134–140CrossRefPubMed

29.

Pereira JR, Valle AL, Ghizoni JS, Só MVR, Ramos MB, Lorenzoni FC (2013) Evaluation of push-out bond strength of four luting agents and SEM observation of the dentine/fibreglass bond interface. Int Endod J 46:982–992CrossRefPubMed

30.

Monticelli F, Grandini S, Goracci C, Ferrari M (2003) Clinical behavior of translucent-fiber posts: a 2-year prospective study. Int J Prosthodont 16:593–596PubMed

31.

van der Sluis LW, Wu MK, Wesselink PR (2005) A comparison between a smooth wire and a K-file in removing artificially placed dentine debris from root canals in resin blocks during ultrasonic irrigation. Int Endod J 38:593–596CrossRefPubMed

32.

Lui JN, Kuah HG, Chen NN (2007) Effect of EDTA with and without surfactants or ultrasonics on removal of smear layer. J Endod 33:472–475CrossRefPubMed

33.

Doğan H, Çalt S (2001) Effects of chelating agents and sodium hypochlorite on mineral content of root dentin. J Endod 27:578–580CrossRefPubMed

34.

Hu X, Peng Y, Sum C, Ling J (2010) Effects of concentrations and exposure times of sodium hypochlorite on dentin deproteination: attenuated total reflection fourier transform infrared spectroscopy study. J Endod 36:2008–2011CrossRefPubMed

Titel: Final irrigation protocols may affect intraradicular dentin ultrastructure
verfasst von: Márcia Helena Wagner
Ricardo Abreu da Rosa
José Antonio Poli de Figueiredo
Marco Antonio Húngaro Duarte
Jefferson Ricardo Pereira
Marcus Vinicius Reis Só
Publikationsdatum: 25.11.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 7/2017
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-2006-x

Neu im Fachgebiet Zahnmedizin

19.04.2024 | Vorhofflimmern | Nachrichten

Newsletter bestellen

Springer Medizin

Final irrigation protocols may affect intraradicular dentin ultrastructure

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Neu im Fachgebiet Zahnmedizin

Parodontalbehandlung verbessert Prognose bei Katheterablation

Invasive Zahnbehandlung: Wann eine Antibiotikaprophylaxe vor infektiöser Endokarditis schützt

Zell-Organisatoren unter Druck: Mechanismen des embryonalen Zahnwachstums aufgedeckt

Die Oralprophylaxe & Kinderzahnheilkunde umbenannt

Newsletter

Springer Medizin

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 7/2017

Semi-quantitative analysis of salivary gland scintigraphy in Sjögren’s syndrome diagnosis: a first-line tool

Effects of enamel matrix derivative on non-surgical management of peri-implant mucositis: a double-blind randomized clinical trial

Effect of photon-initiated photoacoustic streaming, passive ultrasonic, and sonic irrigation techniques on dentinal tubule penetration of irrigation solution: a confocal microscopic study

Osteogain® loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro

Oral appliance therapy versus nasal continuous positive airway pressure in obstructive sleep apnea: a randomized, placebo-controlled trial on psychological distress

Was molar-incisor hypomineralisation (MIH) present in archeological case series?

Neu im Fachgebiet Zahnmedizin

Parodontalbehandlung verbessert Prognose bei Katheterablation

Invasive Zahnbehandlung: Wann eine Antibiotikaprophylaxe vor infektiöser Endokarditis schützt

Zell-Organisatoren unter Druck: Mechanismen des embryonalen Zahnwachstums aufgedeckt

Die Oralprophylaxe & Kinderzahnheilkunde umbenannt

Newsletter