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Journal of Cancer Research and Clinical Oncology

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01.11.2011 | Original Paper

Gastrokine 1 functions as a tumor suppressor by inhibition of epithelial–mesenchymal transition in gastric cancers

verfasst von: Jung Hwan Yoon, Young Hwi Kang, Yoo Jin Choi, In Soo Park, Suk Woo Nam, Jung Young Lee, Yun Sil Lee, Won Sang Park

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 11/2011

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Abstract

Purpose

Gastrokine 1 (GKN1) plays an important role in the gastric mucosal defense mechanism and also acts as a functional gastric tumor suppressor. The specific aim of this study was to determine the molecular mechanisms underlying GKN1 tumor suppressor activity in the progression of gastric cancers.

Methods

We examined the effect of GKN1 on epithelial–mesenchymal transition (EMT) and cell migration in GKN1-transfected and recombinant GKN1-treated AGS gastric cancer cells using in vitro wound healing, microchemotaxis, and invasion assays.

Results

In GKN1-transfected AGS cells, we observed inhibition of cell migration and invasion in wound healing, transwell and Matrigel assay. Also, GKN1-transfected and recombinant GKN1-treated AGS cells showed decreased levels of ROS and expression of phosphatidylinositol 3-kinase (PI3K)/Akt pathway proteins, concomitant with re-expression of E-cadherin and decreased expression of cytoplasmic and nuclear expression of β-catenin, slug, snail, fibronectin, and vimentin.

Conclusions

These data suggest that the GKN1 gene may play an important role in the progression of sporadic gastric cancers via inhibition of EMT and cancer cell migration.

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Titel: Gastrokine 1 functions as a tumor suppressor by inhibition of epithelial–mesenchymal transition in gastric cancers
verfasst von: Jung Hwan Yoon
Young Hwi Kang
Yoo Jin Choi
In Soo Park
Suk Woo Nam
Jung Young Lee
Yun Sil Lee
Won Sang Park
Publikationsdatum: 01.11.2011
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 11/2011
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-011-1051-8

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Springer Medizin

Gastrokine 1 functions as a tumor suppressor by inhibition of epithelial–mesenchymal transition in gastric cancers

Abstract

Purpose

Methods

Results

Conclusions

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Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

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