Background
Materials and methods
Human L5 study
Biochemical analysis of mouse blood plasma
Analysis of cellular senescence and DNA damage in mouse aortas
Analysis of cellular senescence in cultured BAECs
Western blot analysis of lectin-like oxidized LDL receptor-1 (LOX-1) and tumor necrosis factor-α (TNF-α)
Results
The increased percent of L5 in total LDL (L5%) in MetS patients is more pronounced in men than in women
Men | Women | Total | ||||
---|---|---|---|---|---|---|
Control (n = 11) | MetS (n = 15) | Control (n = 15) | MetS (n = 15) | Control (n = 26) | MetS (n = 30) | |
Age (years) | 41.2 ± 9.8 | 48.9 ± 7.1* | 49.3 ± 15.7 | 53.2 ± 4.4 | 45.9 ± 13.9 | 51.1 ± 6.2* |
Waist (cm) | 88.5 ± 10.6 | 106.4 ± 12.9** | 74.4 ± 8.0
#
| 106.2 ± 15.2** | 80.4 ± 11.5 | 106.3 ± 13.9** |
SBP (mmHg) | 121.3 ± 9.6 | 136.2 ± 16.5* | 120.1 ± 26.8 | 120.2 ± 14.8 | 120.6 ± 21.0 | 127.5 ± 17.3 |
DBP (mmHg) | 81.5 ± 10.2 | 83.4 ± 9.8 | 73.0 ± 14.6 | 74.5 ± 9.6 | 76.6 ± 13.4 | 78.6 ± 10.5 |
Glucose (mg/dL) | 89.2 ± 7.7 | 142.7 ± 59.1** | 85.1 ± 7.4 | 102.4 ± 19.4** | 86.9 ± 7.6 | 122.5 ± 47.8** |
TC (mg/dL) | 232.0 ± 47.8 | 196.9 ± 48.0 | 224.9 ± 60.0 | 220.0 ± 59.1 | 227.9 ± 54.2 | 208.5 ± 54.2 |
TG (mg/dL) | 151.8 ± 95.0 | 180.9 ± 151.8 | 106.3 ± 95.5 | 144.1 ± 59.0* | 125.6 ± 96.2 | 162.5 ± 114.7 |
HDL-C (mg/dL) | 52.7 ± 15.8 | 44.0 ± 12.2 | 62.1 ± 13.7 | 54.0 ± 19.2 | 58.1 ± 15.1 | 49.0 ± 16.6* |
LDL-C (mg/dL) | 149.0 ± 37.9 | 120.9 ± 45.3 | 142.3 ± 52.1 | 143.9 ± 41.2 | 145.2 ± 45.9 | 132.8 ± 44.0 |
L5 (%) | 4.1 ± 5.3 | 16.9 ± 14.6** | 5.1 ± 5.6 | 10.1 ± 8.5* | 4.7 ± 5.4 | 13.5 ± 12.2** |
Male db/db mice show increased DNA damage and aortic senescence
Male | Female | |||
---|---|---|---|---|
C57B6/J#(n = 8) | db/db
#
(n = 8) | C57B6/J (n = 6) | db/db(n = 5) | |
Body weight (g) | 36.50 ± 1.46 | 62.50 ± 3.33** | 35.00 ± 0.84 | 57.80 ± 4.84** |
Glucose (mg/dL) | 206.88 ± 10.74 | 398.00 ± 19.89** | 219.17 ± 9.04 | 307.20 ± 41.11* |
TC (mg/dL) | 74.63 ± 4.69 | 188.88 ± 16.00** | 73.17 ± 2.23 | 183.60 ± 10.80** |
TG (mg/dL) | 47.13 ± 5.78 | 52.88 ± 6.24 | 41.33 ± 8.10 | 49.40 ± 7.05 |
HDL-C (mg/dL) | 51.00 ± 3.29 | 97.25 ± 8.24** | 50.83 ± 2.82 | 89.60 ± 7.92** |
LDL-C (mg/dL) | 14.20 ± 1.89 | 81.05 ± 11.29** | 14.07 ± 4.08 | 84.12 ± 6.04** |