Background
Materials and methods
Tissue specimens
Source | Samples | Quantity | No |
---|---|---|---|
Fresh tissues | Normal | 10 | N01,N02……N10 |
PHPT | 12 | P01,P02…….P12 | |
paraffin-embedded tissues | Normal | 11 | N11,N12……N21 |
PHPT | 31 | P13,P14……P43 |
RNA-Sequencing and data processing
PPI network construction and analysis
Whole-Genome Bisulfite Sequencing (WGBS) and data analysis
Real-time quantitative polymerase chain reaction (qRT-PCR)
Primer Name | Primer sequences | Product size |
---|---|---|
POMC-F | CTCCCGAGACAGAGCCTCA | 168 bp |
POMC-R | ACTCCAGCAGGTTGCTTTCC | |
GAPDH-F | CCATGGGTGGAATCATATTGGA | 139 bp |
GAPDH-R | TCAACGGATTTGGTCGTATTGG |
Western blot
Immunohistochemistry (IHC)
Methylation-specific-solymerase chain reaction (MS-PCR)
Primer name | Primer sequences | Product size |
---|---|---|
POMC-M-F | TAGTTTTTAAATAATGGGGAAATCG | 141 bp |
POMC-M-R | AACAACCTCTAAAATCGTTAAAACG | |
POMC-U-F | ATAGTTTTTAAATAATGGGGAAATTG | 140 bp |
POMC-U-R | CAACCTCTAAAATCATTAAAACAAA |