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Journal of Diabetes & Metabolic Disorders

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17.02.2021 | Research article

Gene set enrichment analysis of PPAR-γ regulators from Murraya odorata Blanco

verfasst von: Prarambh SR Dwivedi, V. P. Rasal, Ekta Kotharkar, Shailaja Nare, Pukar Khanal

Erschienen in: Journal of Diabetes & Metabolic Disorders | Ausgabe 1/2021

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Abstract

Background

Peroxisome proliferator-activated receptor gamma (PPAR-γ) is reported to regulate insulin sensitivity and progression of Type 2 diabetes mellitus (T2DM). Hence the present study is aimed to identify PPAR-γ regulators from Murraya odorata Blanco and predict their role to manage T2DM.

Methods

Multiple in-silico tools and databases like SwissTargetPrediction, ADVERPred, PubChem, and MolSoft, were used to retrieve the information related to bioactives, targets, druglikeness character, and probable side effects as applicable. Similarly, the Kyoto Encyclopedia of Genes and Genomes (KEGG) database was used to identify the regulated pathways. Further, the bioactives-protein-pathways network interaction was constructed using Cytoscape. Finally, molecular docking was performed using Autodock4.

Results

Twenty-five bioactives were shortlisted in which six were predicted as PPAR-γ modulators. Among them, stigmasterol was predicted to possess the best binding affinity towards PPAR-γ and possessed no side effects. Similarly, n-hexadecanoic acid was predicted to modulate the highest number of proteins, and protein CD14 was targeted by the highest number of bioactives. Further, the PI3K-Akt pathway was predicted as the maximum modulated genes.

Conclusions

The anti-diabetic property of the Murraya odorata Blanco of fruit pulp may be due to the presence of n-hexadecanoic acid and stigmasterol; may also involve in the regulation of the PI3K-Akt pathway which needs further investigated by in-vitro and in-vivo protocols.

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Titel: Gene set enrichment analysis of PPAR-γ regulators from Murraya odorata Blanco
verfasst von: Prarambh SR Dwivedi
V. P. Rasal
Ekta Kotharkar
Shailaja Nare
Pukar Khanal
Publikationsdatum: 17.02.2021
Verlag: Springer International Publishing
Erschienen in: Journal of Diabetes & Metabolic Disorders / Ausgabe 1/2021
Elektronische ISSN: 2251-6581
DOI: https://doi.org/10.1007/s40200-021-00754-x

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