Skip to main content
Erschienen in: Archives of Virology 6/2014

01.06.2014 | Brief Report

Genetic variation analysis of apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China

Erschienen in: Archives of Virology | Ausgabe 6/2014

Einloggen, um Zugang zu erhalten

Abstract

In this study, we generated sequences of the apple chlorotic leaf spot virus (ACLSV) coat protein (CP) gene. Genetic variation and phylogenetic analyses were carried out on these sequences along with others reported previously. ACLSV populations clustered into four types: in three of the four types, combinations of three amino acids at positions of 40, 75 and 79 were conserved. The fourth phylogenetic type, newly identified here, was characterized by co-variation of Ser40-Tyr75-Ser79. Statistically significant genetic differentiation and infrequent gene flow were detected among the four types. Two natural recombinants were detected for the first time among ACLSV isolates/genotypes from China.
Anhänge
Nur mit Berechtigung zugänglich
Literatur
1.
Zurück zum Zitat Al Rwahnih M, Turturo C, Minafra A, Saldarelli P, Myrta A, Pallás V, Savino V (2004) Molecular variability of Apple chlorotic leaf spot virus in different hosts and geographical regions. J Plant Pathol 86:117–122 Al Rwahnih M, Turturo C, Minafra A, Saldarelli P, Myrta A, Pallás V, Savino V (2004) Molecular variability of Apple chlorotic leaf spot virus in different hosts and geographical regions. J Plant Pathol 86:117–122
2.
Zurück zum Zitat Cai Y, Xiang BC, Xi DH, Liu SX, Du YJ (2005) Cloning and prokaryotic expression of CP gene of Apple chlorotic leaf spot virus Kuerle isolate and preparation of its specific antiserum. J Agric Biotechnol 13:533–537 (in Chinese) Cai Y, Xiang BC, Xi DH, Liu SX, Du YJ (2005) Cloning and prokaryotic expression of CP gene of Apple chlorotic leaf spot virus Kuerle isolate and preparation of its specific antiserum. J Agric Biotechnol 13:533–537 (in Chinese)
3.
Zurück zum Zitat Candresse T, Lanneau M, Revers F, Macquaire G, German S, Grasseau N, Dunez J, Malinowski T (1995) An immunocapture PCR assay adapted to the detection and the analysis of the molecular variability of the Apple chlorotic leaf spot virus. Acta Hortic 386:136–147 Candresse T, Lanneau M, Revers F, Macquaire G, German S, Grasseau N, Dunez J, Malinowski T (1995) An immunocapture PCR assay adapted to the detection and the analysis of the molecular variability of the Apple chlorotic leaf spot virus. Acta Hortic 386:136–147
4.
Zurück zum Zitat Cieślińska M, Malinowski T, Zawadzka BJ (1995) Studies on several strains of Apple chlorotic leaf spot virus (ACLSV) isolated from different fruit tree species. Acta Hortic 386:63–71 Cieślińska M, Malinowski T, Zawadzka BJ (1995) Studies on several strains of Apple chlorotic leaf spot virus (ACLSV) isolated from different fruit tree species. Acta Hortic 386:63–71
6.
Zurück zum Zitat Desvignes JC, Boyé R (1989) Different diseases caused by the chlorotic leaf spot virus on the fruit trees. Acta Hortic 235:31–38 Desvignes JC, Boyé R (1989) Different diseases caused by the chlorotic leaf spot virus on the fruit trees. Acta Hortic 235:31–38
7.
Zurück zum Zitat Dunez J, Marenaud G, Delbos RP, Lansac M (1972) Variability of symptoms induced by the Apple chlorotic leaf spot (CLSV). A type of CLSV probably responsible for bark split disease of prune trees. Plant Dis Rep 56:293–295 Dunez J, Marenaud G, Delbos RP, Lansac M (1972) Variability of symptoms induced by the Apple chlorotic leaf spot (CLSV). A type of CLSV probably responsible for bark split disease of prune trees. Plant Dis Rep 56:293–295
8.
Zurück zum Zitat German-Retana S, Bergey B, Delbos RP, Candresse T, Dunez J (1997) Complete nucleotide sequence of the genome of a severe cherry isolate of apple chlorotic leaf spot trichovirus (ACLSV). Arch Virol 142:833–841PubMedCrossRef German-Retana S, Bergey B, Delbos RP, Candresse T, Dunez J (1997) Complete nucleotide sequence of the genome of a severe cherry isolate of apple chlorotic leaf spot trichovirus (ACLSV). Arch Virol 142:833–841PubMedCrossRef
9.
Zurück zum Zitat German S, Candresse T, Lanneau M, Huet JC, Pernollet JC, Dunez J (1990) Nucleotide sequence and genomic organization of Apple chlorotic leaf spot closterovirus. Virology 179:104–112PubMedCrossRef German S, Candresse T, Lanneau M, Huet JC, Pernollet JC, Dunez J (1990) Nucleotide sequence and genomic organization of Apple chlorotic leaf spot closterovirus. Virology 179:104–112PubMedCrossRef
10.
Zurück zum Zitat German S, Delbos RP, Candresse T, Lanneau M, Dunez J (1997) Complete nucleotide sequence of the genome of a severe cherry isolate of Apple chlorotic leaf spot trichovirus (ACLSV). Arch Virol 142:833–841CrossRef German S, Delbos RP, Candresse T, Lanneau M, Dunez J (1997) Complete nucleotide sequence of the genome of a severe cherry isolate of Apple chlorotic leaf spot trichovirus (ACLSV). Arch Virol 142:833–841CrossRef
12.
Zurück zum Zitat Hudson RR, Boos DD, Kaplan NL (1992) A statistical test for detecting geographic subdivision. Mol Biol Evol 9:138–151PubMed Hudson RR, Boos DD, Kaplan NL (1992) A statistical test for detecting geographic subdivision. Mol Biol Evol 9:138–151PubMed
13.
Zurück zum Zitat Jelkmann W (1996) The nucleotide sequence of a strain of Apple chlorotic leaf spot virus (ACLSV) responsible for plum pseudopox and its relation to an apple and plum bark split strain. Phytopathology 86:101 Jelkmann W (1996) The nucleotide sequence of a strain of Apple chlorotic leaf spot virus (ACLSV) responsible for plum pseudopox and its relation to an apple and plum bark split strain. Phytopathology 86:101
14.
Zurück zum Zitat Jelkmann W, Kunze L (1995) Plum pseudopox in German prune after infection with an isolate of Apple chlorotic leafspot virus causing plum line pattern. Acta Hortic 386:122–125 Jelkmann W, Kunze L (1995) Plum pseudopox in German prune after infection with an isolate of Apple chlorotic leafspot virus causing plum line pattern. Acta Hortic 386:122–125
15.
Zurück zum Zitat King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2012) Virus taxonomy: classification and nomenclature of viruses: ninth report of the international committee on taxonomy of viruses. Elsevier, San Diego King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (2012) Virus taxonomy: classification and nomenclature of viruses: ninth report of the international committee on taxonomy of viruses. Elsevier, San Diego
16.
Zurück zum Zitat Krizbai L, Ember I, Nemeth M, Kôlber M, Pasquini G, Faggioli F (2001) Characterization of Hungarian isolates of Apple chlorotic leafspot virus. Acta Hortic 1:291–306 Krizbai L, Ember I, Nemeth M, Kôlber M, Pasquini G, Faggioli F (2001) Characterization of Hungarian isolates of Apple chlorotic leafspot virus. Acta Hortic 1:291–306
17.
Zurück zum Zitat Liu FC, Wang HY (1989) On latent viruses of apple in China II: indexing of latent viruses in apple scion varieties and dwarf rootstocks. Acta Phytopathol Sinica 19:193–197 (in Chinese) Liu FC, Wang HY (1989) On latent viruses of apple in China II: indexing of latent viruses in apple scion varieties and dwarf rootstocks. Acta Phytopathol Sinica 19:193–197 (in Chinese)
18.
Zurück zum Zitat Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452PubMedCrossRef Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452PubMedCrossRef
19.
Zurück zum Zitat Lemey P, Salemi M, Vandamme A (2009) The phylogenetic handbook: a practical approach to phylogenetic analysis and hypothesis testing, 2nd edn. Cambridge University Press, CambridgeCrossRef Lemey P, Salemi M, Vandamme A (2009) The phylogenetic handbook: a practical approach to phylogenetic analysis and hypothesis testing, 2nd edn. Cambridge University Press, CambridgeCrossRef
20.
Zurück zum Zitat Magome H, Yoshikawa N, Takahashi T, Ito T, Miyakawa T (1997) Molecular variability of the genomes of capilloviruses from apple, Japanese pear, European pear, and citrus trees. Phytopathology 87:389–396PubMedCrossRef Magome H, Yoshikawa N, Takahashi T, Ito T, Miyakawa T (1997) Molecular variability of the genomes of capilloviruses from apple, Japanese pear, European pear, and citrus trees. Phytopathology 87:389–396PubMedCrossRef
21.
Zurück zum Zitat Marini DB, Gibson PG, Scott SW (2008) The complete nucleotide sequence of an isolate of apple chlorotic leaf spot virus from peach (Prunus persica (L.) Batch). Arch Virol 153:1003–1005PubMedCrossRef Marini DB, Gibson PG, Scott SW (2008) The complete nucleotide sequence of an isolate of apple chlorotic leaf spot virus from peach (Prunus persica (L.) Batch). Arch Virol 153:1003–1005PubMedCrossRef
22.
Zurück zum Zitat Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26:2462–2463PubMedCentralPubMedCrossRef Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26:2462–2463PubMedCentralPubMedCrossRef
23.
Zurück zum Zitat Nagy PD (2008) Recombination in plant RNA viruses. In: Roossinck M (ed) Plant virus evolution. Springer, Berlin, pp 133–156CrossRef Nagy PD (2008) Recombination in plant RNA viruses. In: Roossinck M (ed) Plant virus evolution. Springer, Berlin, pp 133–156CrossRef
24.
Zurück zum Zitat Németh M (1986) Viruses, mycoplasma and rickettsia diseases of fruit trees. Akadémiai Kiadó, Budapest Németh M (1986) Viruses, mycoplasma and rickettsia diseases of fruit trees. Akadémiai Kiadó, Budapest
25.
Zurück zum Zitat Niu FQ, Pan S, Wu ZJ, Jiang DM, Li SF (2012) Complete nucleotide sequences of the genomes of two isolates of apple chlorotic leaf spot virus from peach (Prunus persica) in China. Arch Virol 157:783–786PubMedCrossRef Niu FQ, Pan S, Wu ZJ, Jiang DM, Li SF (2012) Complete nucleotide sequences of the genomes of two isolates of apple chlorotic leaf spot virus from peach (Prunus persica) in China. Arch Virol 157:783–786PubMedCrossRef
26.
Zurück zum Zitat Pasquini G, Faggioli F, Pilotti M, Lumia V, Barba M (1998) Characterisation of Apple chlorotic leaf spot virus isolates from Italy. Acta Hortic 472:195–199 Pasquini G, Faggioli F, Pilotti M, Lumia V, Barba M (1998) Characterisation of Apple chlorotic leaf spot virus isolates from Italy. Acta Hortic 472:195–199
27.
Zurück zum Zitat Rana T, Chandel V, Kumar Y, Ram R, Hallan V, Zaidi AA (2010) Molecular variability analyses of Apple chlorotic leaf spot virus capsid protein. J Biosci 35:605–615PubMedCrossRef Rana T, Chandel V, Kumar Y, Ram R, Hallan V, Zaidi AA (2010) Molecular variability analyses of Apple chlorotic leaf spot virus capsid protein. J Biosci 35:605–615PubMedCrossRef
28.
Zurück zum Zitat Sato K, Yoshikawa N, Takahashi T (1993) Complete nucleotide sequence of the genome of an apple isolate of Apple chlorotic leaf spot virus. J Gen Virol 74:1927–1931PubMedCrossRef Sato K, Yoshikawa N, Takahashi T (1993) Complete nucleotide sequence of the genome of an apple isolate of Apple chlorotic leaf spot virus. J Gen Virol 74:1927–1931PubMedCrossRef
29.
Zurück zum Zitat Song YS, Hong N, Wang LP, Hu HJ, Tian R, Xu WX, Ding F, Wang GP (2011) Molecular and serological diversity in Apple chlorotic leaf spot virus from sand pear (Pyrus pyrifolia) in China. Eur J Plant Pathol 130:183–196CrossRef Song YS, Hong N, Wang LP, Hu HJ, Tian R, Xu WX, Ding F, Wang GP (2011) Molecular and serological diversity in Apple chlorotic leaf spot virus from sand pear (Pyrus pyrifolia) in China. Eur J Plant Pathol 130:183–196CrossRef
30.
Zurück zum Zitat Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRef Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739PubMedCentralPubMedCrossRef
31.
Zurück zum Zitat Wu ZB, Ku HM, Chen YK, Chang CJ, Jan FJ (2010) Biological and molecular characterization of Apple chlorotic leaf spot virus causing chlorotic leaf spot on pear (Pyrus pyrifolia) in Taiwan. HortScience 45:1073–1078 Wu ZB, Ku HM, Chen YK, Chang CJ, Jan FJ (2010) Biological and molecular characterization of Apple chlorotic leaf spot virus causing chlorotic leaf spot on pear (Pyrus pyrifolia) in Taiwan. HortScience 45:1073–1078
32.
Zurück zum Zitat Yaegashi H, Isogai M, Tajima H, Sano T, Yoshikawa N (2007) Combinations of two amino acids (Ala40 and Phe75 or Ser40 and Tyr75) in the coat protein of apple chlorotic leaf spot virus are crucial for infectivity. J Gen Virol 88:2611–2618PubMedCrossRef Yaegashi H, Isogai M, Tajima H, Sano T, Yoshikawa N (2007) Combinations of two amino acids (Ala40 and Phe75 or Ser40 and Tyr75) in the coat protein of apple chlorotic leaf spot virus are crucial for infectivity. J Gen Virol 88:2611–2618PubMedCrossRef
33.
Zurück zum Zitat Yoshikawa N, Takahashi T (1988) Properties of RNAs and proteins of Apple stem grooving and Apple chlorotic leaf spot viruses. J Gen Virol 88:2611–2618 Yoshikawa N, Takahashi T (1988) Properties of RNAs and proteins of Apple stem grooving and Apple chlorotic leaf spot viruses. J Gen Virol 88:2611–2618
34.
Zurück zum Zitat Zheng YY, Wang GP, Hong N, Song YS, You H (2007) Partial molecular characterization of Apple chlorotic leaf spot virus from peach and apple trees and prokaryotic expression for cp gene. Acta Phytopathol Sinica 37:356–361 (in Chinese) Zheng YY, Wang GP, Hong N, Song YS, You H (2007) Partial molecular characterization of Apple chlorotic leaf spot virus from peach and apple trees and prokaryotic expression for cp gene. Acta Phytopathol Sinica 37:356–361 (in Chinese)
Metadaten
Titel
Genetic variation analysis of apple chlorotic leaf spot virus coat protein reveals a new phylogenetic type and two recombinants in China
Publikationsdatum
01.06.2014
Erschienen in
Archives of Virology / Ausgabe 6/2014
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1927-9

Weitere Artikel der Ausgabe 6/2014

Archives of Virology 6/2014 Zur Ausgabe

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Battle of Experts: Sport vs. Spritze bei Adipositas und Typ-2-Diabetes

11.05.2024 DDG-Jahrestagung 2024 Kongressbericht

Im Battle of Experts traten zwei Experten auf dem Diabeteskongress gegeneinander an: Die eine vertrat die Auffassung „Sport statt Spritze“ bei Adipositas und Typ-2-Diabetes, der andere forderte „Spritze statt Sport!“ Am Ende waren sie sich aber einig: Die Kombination aus beidem erzielt die besten Ergebnisse.

Triglyzeridsenker schützt nicht nur Hochrisikopatienten

10.05.2024 Hypercholesterinämie Nachrichten

Patienten mit Arteriosklerose-bedingten kardiovaskulären Erkrankungen, die trotz Statineinnahme zu hohe Triglyzeridspiegel haben, profitieren von einer Behandlung mit Icosapent-Ethyl, und zwar unabhängig vom individuellen Risikoprofil.

Gibt es eine Wende bei den bioresorbierbaren Gefäßstützen?

In den USA ist erstmals eine bioresorbierbare Gefäßstütze – auch Scaffold genannt – zur Rekanalisation infrapoplitealer Arterien bei schwerer PAVK zugelassen worden. Das markiert einen Wendepunkt in der Geschichte dieser speziellen Gefäßstützen.

Vorsicht, erhöhte Blutungsgefahr nach PCI!

10.05.2024 Koronare Herzerkrankung Nachrichten

Nach PCI besteht ein erhöhtes Blutungsrisiko, wenn die Behandelten eine verminderte linksventrikuläre Ejektionsfraktion aufweisen. Das Risiko ist umso höher, je stärker die Pumpfunktion eingeschränkt ist.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.