Background
Results
Strain identification by 16S rRNA gene sequencing
Genomic features of strain DMB3-Bh1
MIGS ID | Property | Term |
---|---|---|
MIGS-31 | Finishing quality | High quality draft |
MIGS-28 | Libraries used | Paired end ~330 bp |
MIGS-29 | Sequencing platforms | Illumina HiSeq 1000 |
MIGS-31.2 | Sequencing coverage | 1145.46x |
MIGS-30 | Assemblers | CLC Bio Workbench v6.0.4 |
MIGS-32 | Gene calling method | Prodigal 1.4, GenePRIMP |
Genbank ID | AURW01000000 | |
---|---|---|
NCBI project ID | PRJNA210599 | |
MIGS-13 | Project relevance | Virulence factor, phages |
Attribute | Value | % of Total |
---|---|---|
Genome size (bp) | 2,810,255 | |
DNA coding region (bp) | 2,364,894 | 84.15 |
DNA G+C content (bp) | 460,557 + 457,273 | 32.7 |
Total genes | 2681 | 84.46 |
RNA genes | 58 | 0.31 |
rRNA operons | 2 | 0.16 |
Protein-coding genes | 2623 | |
Pseudo genes | 560 | 10.47 |
Genes with function prediction | 2121 | 73.99 |
Genes in paralog clusters | 181 | 7.28 |
Genes assigned to COGs | 2474 | 92.28 |
Genes with transmembrane helices | 8 | 0.28 |
Genes involved in virulence, disease and defense
Code | Value | % age | Description |
---|---|---|---|
J | 149 | 6.7 | Translation, ribosomal structure and biogenesis |
A | 0 | 0.0 | RNA processing and modification |
K | 129 | 5.9 | Transcription |
L | 115 | 5.3 | Replication, recombination and repair |
B | 0 | 0.0 | Chromatin structure and dynamics |
D | 28 | 1.3 | Cell cycle control, mitosis and meiosis |
Y | 0 | 0.0 | Nuclear structure |
V | 32 | 1.5 | Defense mechanisms |
T | 133 | 6.1 | Signal transduction mechanisms |
M | 119 | 5.5 | Cell wall/membrane biogenesis |
N | 75 | 3.5 | Cell motility |
Z | 0 | 0.0 | Cytoskeleton |
W | 0 | 0.0 | Extracellular structures |
U | 46 | 2.1 | Intracellular trafficking and secretion, and vesicular transport |
O | 70 | 3.2 | Posttranslational modification, protein turnover, chaperones |
C | 142 | 6.5 | Energy production and conversion |
G | 113 | 5.2 | Carbohydrate transport and metabolism |
E | 252 | 11.6 | Amino acid transport and metabolism |
F | 65 | 3.0 | Nucleotide transport and metabolism |
H | 99 | 4.6 | Coenzyme transport and metabolism |
I | 44 | 2.0 | Lipid transport and metabolism |
P | 125 | 5.8 | Inorganic ion transport and metabolism |
Q | 31 | 1.4 | Secondary metabolites biosynthesis, transport and catabolism |
R | 243 | 11.2 | General function prediction only |
S | 161 | 7.4 | Function unknown |
– | 565 | 22.5 | Not in COGs |
Phages, prophages, transposable elements, plasmids
Function based comparative genomic analysis
Discussion
Conclusions
Methods
Isolation of bacterial strains and growth conditions
MIGS ID | Property | Term | Evidence code |
---|---|---|---|
Current classification | Domain Bacteria
| [34] | |
Phylum Firmicutes
| |||
Class Bacilli
| |||
Order Bacillales
| |||
Family Staphylococcaceae
| |||
Genus Staphylococcus
| |||
Species Staphylococcus xylosus
| [40] | ||
Strain DMB3-Bh1 | Present study | ||
Gram stain | Positive | IDA | |
Cell shape | Coccus | IDA | |
Motility | Non-motile | IDA | |
Sporulation | Non-sporulating | IDA | |
Temperature range | 20-45 °C | IDA | |
Optimum temperature | 37 °C | IDA | |
Salinity | 3% NaCl | IDA | |
MIGS-22 | Oxygen requirement | Facultatively anaerobic | IDA |
Carbon source | Glucose, fructose | IDA | |
Energy source | Fructose, trehalose | IDA | |
MIGS-6 | Habitat | Mouse faecal | IDA |
MIGS-15 | Biotic relationship | Free living | IDA |
MIGS-14 | Pathogenicity | Non pathogenic | IDA |
Biosafety level | 1 | [44] | |
Isolation | From mouse faecal sample | IDA | |
MIGS-4 | Geographic location | Chandigarh,India | IDA |
MIGS-5 | Sample collection time | 9th,October 2012 | IDA |
MIGS-4.1 MIGS-4.2 | Latitude–longitude | 30.7660° N, 76.7754° E | IDA |
MIGS-4.3 | Depth | Unknown | IDA |
MIGS-4.4 | Altitude | About sea level | IDA |