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01.04.2013 | Original Article

Glomerular expression of connexin 40 and connexin 43 in rat experimental glomerulonephritis

verfasst von: Tetsuo Morioka, Shinichi Okada, Masaaki Nameta, Fadia Kamal, Nadia T. Yanakieva-Georgieva, Jian Yao, Ayako Sato, Honglan Piao, Takashi Oite

Erschienen in: Clinical and Experimental Nephrology | Ausgabe 2/2013

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Abstract

Background

Gap junctional intercellular communication is thought to play an important role in the maintenance of cell differentiation and homeostasis. Gap junctions connect glomerular mesangial cells to each other. In this study, we examined the glomerular expression of connexins (Cxs) 40 and 43 at both the protein and transcript levels in anti-Thy1.1 glomerulonephritis (GN).

Methods

Anti-Thy1.1 GN was induced by intravenous injection of anti-Thy1.1 monoclonal antibody 1-22-3. Cx protein expression was examined by immunofluorescence, immunoelectron microscopy, and Western blotting. Changes in mRNA levels were detected by real-time reverse transcriptase–polymerase chain reaction.

Results

Cx40 was detected in mesangial cells in normal rat glomeruli; its expression was reduced on days 3 and 7 and recovered to normal on day 14 following GN induction. Cx43 was detected in mesangial cells and podocytes in normal rat glomeruli, and its expression did not change during the disease course of GN. Expression of Cx40 and Cx43 was also detected in extraglomerular mesangial cells; this expression did not change during the disease course. Opposing patterns of expression between Cx40 and smooth muscle actin (SMA) were observed with double-immunofluorescence labeling. SMA is a differentiation marker of mesangial cells; it is often expressed during proliferation but not under physiological conditions.

Conclusion

These results suggest that Cx40 expression in mesangial cells is related to mesangial cell regeneration. Thus, Cx expression regulation could be a therapeutic target for glomerular diseases.

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Titel: Glomerular expression of connexin 40 and connexin 43 in rat experimental glomerulonephritis
verfasst von: Tetsuo Morioka
Shinichi Okada
Masaaki Nameta
Fadia Kamal
Nadia T. Yanakieva-Georgieva
Jian Yao
Ayako Sato
Honglan Piao
Takashi Oite
Publikationsdatum: 01.04.2013
Verlag: Springer Japan
Erschienen in: Clinical and Experimental Nephrology / Ausgabe 2/2013
Print ISSN: 1342-1751
Elektronische ISSN: 1437-7799
DOI: https://doi.org/10.1007/s10157-012-0687-2

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