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Diabetologia
Erschienen in: Diabetologia 2/2003

01.02.2003 | Article

Glucose oversupply increases Δ9-desaturase expression and its metabolites in rat skeletal muscle

verfasst von: B. Houdali, H. G. Wahl, M. Kresi, V. Nguyen, M. Haap, F. Machicao, H. P. T. Ammon, W. Renn, E. D. Schleicher, H.-U. Häring

Erschienen in: Diabetologia | Ausgabe 2/2003

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Abstract

Aim/hypothesis

Previous studies have shown that prolonged glucose infusion causes insulin resistance and triglyceride accumulation in rat skeletal muscle. In this study, we investigated a possible relationship between insulin resistance and the composition of different accumulated lipid fractions in rat skeletal muscle.

Methods

Continuous glucose infusion was carried out in rats for 7 days. Lipids were extracted from skeletal muscle, separated by thin layer chromatography and fatty acid composition of phospholipids, triglycerides, diglycerides, free fatty acids and cholesterol esters fractions was analysed by gas chromatography. Δ9-Desaturase mRNA was measured by real time polymerase chain reaction. The enzyme activity was measured in the microsomal fractions.

Results

Prolonged glucose infusion (5 days) increased the relative content of palmitoleic acid (16:1 N7) several-fold (2.3- to 5.8-fold) in four out of five lipid fractions and enhanced oleic acid (18:1 N9) two-fold in three lipid fractions suggesting increased Δ9-desaturase activity while the content of several polyunsaturated fatty acids was reduced. In parallel, Δ9-Desaturase mRNA contents and enzyme activities in skeletal muscle were increased 10-fold, 75-fold, 2.6-fold and 7.7-fold after 2 and 5 days of glucose infusion, respectively.

Conclusion/interpretation

Our results suggest that long-term glucose oversupply induces a rapid increase in Δ9-desaturase expression and enzyme activity in skeletal muscle which leads to fast and specific changes in fatty acid metabolism possibly contributing to the insulin resistance in this animal model.
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Metadaten
Titel
Glucose oversupply increases Δ9-desaturase expression and its metabolites in rat skeletal muscle
verfasst von
B. Houdali
H. G. Wahl
M. Kresi
V. Nguyen
M. Haap
F. Machicao
H. P. T. Ammon
W. Renn
E. D. Schleicher
H.-U. Häring
Publikationsdatum
01.02.2003
Verlag
Springer-Verlag
Erschienen in
Diabetologia / Ausgabe 2/2003
Print ISSN: 0012-186X
Elektronische ISSN: 1432-0428
DOI
https://doi.org/10.1007/s00125-002-1015-2

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