Discussion
GCT is a rare tumor that can affect various regions of the body, such as the skin, soft tissues, breast, and lungs [
3]. However, GCT is more frequently found in the head and neck region, which accounts for 45% to 65% of all sites affected by the tumor. Of these, 70% are located in the oral cavity, especially the tongue, oral mucosa, and hard palate [
2]. Considering the wide variety of sites affected by the tumor and its variable histological presentation, a correct clinical description is fundamental. Although the etiology of GCT is still controversial, the currently most accepted hypothesis is that the tumor arises from Schwann cells or their precursors [
2,
4]. Immunohistochemical analysis has shown a strong and consistent positivity for protein S-100, a finding supporting the hypothesis that GCT is of peripheral nerve sheath origin [
4].
In the study by Rejas
et al. [
5], the immunoprofile of GCTs showed nerve sheath differentiation, a finding lending support to a neural origin of these tumors and contributing to the establishment of a differential diagnosis between this lesion and other oral granular cell tumors, whether benign or malignant. Vered
et al. [
6] recently tested an extensive panel of antibodies to determine the true origin of this tumor. In most cases, granular cells were strongly and diffusely positive for p75, vimentin, calretinin, NKI/C3, inhibin-α, protein gene product 9.5 (PGP9.5), and protein S-100. However, the authors called attention to the fact that the antibodies stained different tissues. As a consequence, no particular cell type that would be responsible for the histogenetic origin of GCT could be identified.
GCT seems to be more prevalent among women, but a gender preference is not unanimously accepted. The tumor commonly develops between the second and sixth decade of life [
7] and is rare in children [
8]. Clinically, benign GCT manifests as a nodular lesion that is generally asymptomatic and solitary, although cases of multiple lesions have been reported [
7,
9]. The tumor presents as a pink or yellow well delimited lesion that rarely exceeds 3 cm in diameter, is covered by intact mucosa, and usually involves subcutaneous or submucosal tissues. There are reports of painful symptoms during tooth brushing, consumption of spicy foods, and bite trauma [
7].
In the present report, one of our patients reported pain, whereas the tumor was asymptomatic in our other patient. Although the symptomatic lesion showed no ulceration, the deeper location of the tumor may have affected adjacent nerve fibers. In a recent study including 68 cases of GCT, Vered
et al. [
6] observed a strong association between granular cells and skeletal muscle. However, clusters of granular cells around nerve fibers were only observed in nine (21%) patients and replacement of nerves with tumor cells was noted in five of these cases.
Clinically, any nodular lesion involving oral soft tissue can be included in the differential diagnosis. Features such as consistency, color and the possible definition of lesion margins upon palpation may facilitate the establishment of diagnostic hypotheses. Histologically, GCTs are characterized by the proliferation of large polygonal neoplastic cells with cytoplasmic granules, eosinophilic cytoplasm, a small and eccentrically located nucleus, and undefined cytoplasmic limits. In some cases, the epithelium that covers the tumor exhibits pseudoepitheliomatous hyperplasia [
7].
In the oral cavity, granular cells can be found in lesions other than GCT, including ameloblastoma, ameloblastic fibroma, odontogenic fibroma, odontogenic cysts, congenital epulis of the newborn, and oral lichen planus [
10,
11]. In view of the diversity of lesions that contain granular cells, this condition is still a matter of discussion. Marked pseudoepitheliomatous hyperplasia is observed in some cases of GCT, a fact that might lead to a false diagnosis of squamous cell carcinoma. This occurs because an incisional biopsy is performed which only involves the epithelium of the tumor surface without clear definition of granular cells. However, clinical data about the tumor and more detailed information provided by the professional who performed the biopsy may help define the diagnosis.
Although GCT is an uncommon benign neoplasm, cases of malignant GCT have been reported in the literature, including patients with more than one histological type of malignant GCT [
12‐
14]. The coexistence of benign GCT of the tongue and squamous cell carcinoma at the same site has also been reported recently [
15]. In view of this malignant potential, the tumor should be submitted to careful histopathological analysis. Data regarding tumor size, symptoms, rapid progression, invasion of adjacent structures, and the presence of regional and distant metastases are of fundamental importance for the histopathological diagnosis of benign or malignant GCT [
13,
15].
Surgical excision with a safety margin is the treatment of choice for GCT, although this is not always possible because the tumor lacks a capsule, a condition histologically demonstrated by an undefined cell margin. An excisional biopsy was also the treatment of choice in the present cases, but excision with tumor-free margins was not possible in case 1 because of the extent and location of the tumor. Both cases were followed-up for five years and no signs of recurrence were observed.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
ASRC and NCSC analyzed and interpreted the clinical data from our patients and wrote the manuscript. YRC performed the histopathological examination of the lesions. JDA and FB participated in the writing of the manuscript. All authors have read and approved the final version of the manuscript.