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Erschienen in: Journal of Cancer Research and Clinical Oncology 2/2007

01.02.2007 | Original Paper

High-density oligonucleotide microarrays and functional network analysis reveal extended lung carcinogenesis pathway maps and multiple interacting genes in NNK [4-(methylnitrosamino)-1-(3-pyridyle)-1-butanone] induced CD1 mouse lung tumor

verfasst von: Hekmat Osman Abdel-Aziz, Ichiro Takasaki, Yoshiaki Tabuchi, Kazuhiro Nomoto, Yoshihiro Murai, Koichi Tsuneyama, Yasuo Takano

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 2/2007

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Abstract

Purpose

NNK [4-(methylnitrosamino)-1-(3-pyridyle)-1-butanone] is a nicotine-derived nitrosaminoketone contained in tobacco smoke used as a powerful chemical carcinogen for rodent experimental models of pulmonary carcinogenesis. To clarify its carcinogenetic mechanisms, we examined the expression status of 22,625 mouse genes.

Methods

The affymetrix GeneChip mouse expression 430 A arrays have been used in CD1-induced mouse lung tumor. The affected genes were analyzed by Ingenuity pathway analysis to investigate functional network and gene ontology.

Results

A total of 876 genes were found to be differentially expressed at least twofold between NNK-induced tumors and normal lung tissues, 390 up-regulated and 486 down-regulated in these lesions. The functions with the highest P values were related to cellular growth and proliferation (P = 1.71 × 10−4 to 4.10 × 10−2). In addition, we identified canonical pathways for Wnt/β-catenin signaling (P = 0.0338).

Conclusions

These results suggest that application of gene expression profiling may provide an improved strategy for therapeutic targeting of tobacco smoking-induced lung cancer.
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Metadaten
Titel
High-density oligonucleotide microarrays and functional network analysis reveal extended lung carcinogenesis pathway maps and multiple interacting genes in NNK [4-(methylnitrosamino)-1-(3-pyridyle)-1-butanone] induced CD1 mouse lung tumor
verfasst von
Hekmat Osman Abdel-Aziz
Ichiro Takasaki
Yoshiaki Tabuchi
Kazuhiro Nomoto
Yoshihiro Murai
Koichi Tsuneyama
Yasuo Takano
Publikationsdatum
01.02.2007
Verlag
Springer-Verlag
Erschienen in
Journal of Cancer Research and Clinical Oncology / Ausgabe 2/2007
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI
https://doi.org/10.1007/s00432-006-0149-x

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