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Erschienen in:

01.08.2013

High levels of divergent HIV-1 quasispecies in patients with neurological opportunistic infections in China

verfasst von: Yulin Zhang, Feili Wei, Qi Liang, Wei Ding, Luxin Qiao, Fengli Song, Lifeng Liu, Sufang Yang, Ronghua Jin, Jianhua Gu, Ning Li, Dexi Chen

Erschienen in: Journal of NeuroVirology | Ausgabe 4/2013

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Abstract

Despite the fact that the survival of people infected with human immunodeficiency virus (HIV) has improved worldwide because of the increasingly powerful and highly active antiretroviral therapy, opportunistic infections (OIs) of the central nervous system (CNS) remain a serious burden. HIV-1 is capable of entering the CNS through infected peripheral monocytes, but its effect on OIs of CNS remains unclear. In this study, we investigated the characteristics of HIV-1 in acquired immunodeficiency syndrome (AIDS) patients with CNS OIs. A total of 24 patients with CNS OIs and 16 non-CNS OIs (control) cases were selected. These AIDS patients were infected with HIV-1 by paid blood donors in China. HIV-1 loads in plasma and cerebrospinal fluid (CSF) were detected using RT-PCR, and the C2-V5 region of HIV-1 envelope gene was amplified from viral quasispecies isolated from CSF using nested PCR. The CSF HIV-1 load of CNS OIs was higher than that of non-CNS OIs, but plasma HIV-1 load of CNS OIs was not higher than that of non-CNS OIs. The nucleotide sequence of C2-V5 region of the HIV-1 quasispecies isolated from the CSF of CNS OIs had a high diversity, and the HIV-1 quasispecies isolated from the CSF of CNS OIs revealed R5 tropism as 11/25 charge rule. These results suggest that high levels of divergent HIV-1 quasispecies in the CNS probably contribute to opportunistic infections.

Bachis A, Biggio F, Major EO, Mocchetti I (2009) M- and T-tropic HIVs promote apoptosis in rat neurons. J Neuroimmune Pharmacol 4:150–60PubMedCrossRef

Borda JT, Alvarez X, Mohan M, Hasegawa A, Bernardino A, Jean S, Aye P, Lackner AA (2008) CD163, a marker of perivascular macrophages, is up-regulated by microglia in simian immunodeficiency virus encephalitis after haptoglobin-hemoglobin complex stimulation and is suggestive of breakdown of the blood–brain barrier. Am J Pathol 172:725–37PubMedCrossRef

Christo PP, Greco DB, Aleixo AW, Livramento JA (2005) HIV-1 RNA levels in cerebrospinal fluid and plasma and their correlation with opportunistic neurological diseases in a Brazilian AIDS reference hospital. Arq Neuropsiquiatr 63:907–13PubMed

Christo PP, Greco DB, Aleixo AW, Livramento JA (2007) Factors influencing cerebrospinal fluid and plasma HIV-1 RNA detection rate in patients with and without opportunistic neurological disease during the HAART era. BMC Infect Dis 7:147PubMedCrossRef

Christo PP, Vilela Mde C, Bretas TL, Domingues RB, Greco DB, Livramento JA, Teixeira AL (2009) Cerebrospinal fluid levels of chemokines in HIV infected patients with and without opportunistic infection of the central nervous system. J Neurol Sci 287:79–83PubMedCrossRef

Chun H, Hao W, Honghai Z, Ning L, Yasong W, Chen D (2009) CCL3L1 prevents gp120-induced neuron death via the CREB cell signaling pathway. Brain Res 1257:75–88PubMedCrossRef

COHERE (2012) CD4 cell count and the risk of AIDS or death in HIV-infected adults on combination antiretroviral therapy with a suppressed viral load: a longitudinal cohort study from COHERE. PLoS Med 9:e1001194CrossRef

Ginhoux F, Greter M, Leboeuf M, Nandi S, See P, Gokhan S, Mehler MF, Conway SJ, Ng LG, Stanley ER, Samokhvalov IM, Merad M (2010) Fate mapping analysis reveals that adult microglia derive from primitive macrophages. Science 330:841–5PubMedCrossRef

Goodenow MM, Collman RG (2006) HIV-1 coreceptor preference is distinct from target cell tropism: a dual-parameter nomenclature to define viral phenotypes. J Leukoc Biol 80:965–72PubMedCrossRef

Haase AT (1986) Pathogenesis of lentivirus infections. Nature 322:130–6PubMedCrossRef

Jensen MA, van 't Wout AB (2003) Predicting HIV-1 coreceptor usage with sequence analysis. AIDS Rev 5:104–12PubMed

Kaplan JE, Benson C, Holmes KH, Brooks JT, Pau A, Masur H (2009) Guidelines for prevention and treatment of opportunistic infections in HIV-infected adults and adolescents: recommendations from CDC, the National Institutes of Health, and the HIV Medicine Association of the Infectious Diseases Society of America. MMWR Recomm Rep 58: 1–207; quiz CE1-4.

Kim WK, Alvarez X, Fisher J, Bronfin B, Westmoreland S, McLaurin J, Williams K (2006) CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood. Am J Pathol 168:822–34PubMedCrossRef

Liu L, Zhao Q, Wei F, Yuan L, Zhang Y, Qiao L, Shi Y, Li N, Chen D (2012) Genetic analysis of HIV type 1 env gene in cerebrospinal fluid and plasma of infected Chinese paid blood donors. AIDS Res Hum Retroviruses 28:106–9PubMedCrossRef

Martin C, Albert J, Hansson P, Pehrsson P, Link H, Sonnerborg A (1998) Cerebrospinal fluid mononuclear cell counts influence CSF HIV-1 RNA levels. J Acquir Immune Defic Syndr Hum Retrovirol 17:214–9PubMedCrossRef

Masur H, Kaplan JE (2009) New guidelines for the management of HIV-related opportunistic infections. JAMA 301:2378–80PubMedCrossRef

Mocroft AJ, Lundgren JD, D'Armino Monforte A, Ledergerber B, Barton SE, Vella S, Katlama C, Gerstoft J, Pedersen C, Phillips AN (1997) Survival of AIDS patients according to type of AIDS-defining event. The AIDS in Europe Study Group. Int J Epidemiol 26:400–7PubMedCrossRef

Perry VH, Nicoll JA, Holmes C (2010) Microglia in neurodegenerative disease. Nat Rev Neurol 6:193–201PubMedCrossRef

Riveiro-Barciela M, Falco V, Burgos J, Curran A, Van den Eynde E, Navarro J, Villar Del Saz S, Ocana I, Ribera E, Crespo M, Pahissa A (2013) Neurological opportunistic infections and neurological immune reconstitution syndrome: impact of one decade of highly active antiretroviral treatment in a tertiary hospital. HIV Med 14:21–30

Saitoh A, Fenton T, Alvero C, Fletcher CV, Spector SA (2007) Impact of nucleoside reverse transcriptase inhibitors on mitochondria in human immunodeficiency virus type 1-infected children receiving highly active antiretroviral therapy. Antimicrob Agents Chemother 51:4236–42PubMedCrossRef

Soulas C, Conerly C, Kim WK, Burdo TH, Alvarez X, Lackner AA, Williams KC (2011) Recently infiltrating MAC387(+) monocytes/macrophages a third macrophage population involved in SIV and HIV encephalitic lesion formation. Am J Pathol 178:2121–35PubMedCrossRef

Soulas C, Donahue RE, Dunbar CE, Persons DA, Alvarez X, Williams KC (2009) Genetically modified CD34+ hematopoietic stem cells contribute to turnover of brain perivascular macrophages in long-term repopulated primates. Am J Pathol 174:1808–17PubMedCrossRef

Soulie C, Fourati S, Lambert-Niclot S, Tubiana R, Canestri A, Girard PM, Katlama C, Morand-Joubert L, Calvez V, Marcelin AG (2010) HIV genetic diversity between plasma and cerebrospinal fluid in patients with HIV encephalitis. AIDS 24:2412–4PubMed

Spudich S, Gonzalez-Scarano F (2012) HIV-1-related central nervous system disease: current issues in pathogenesis, diagnosis, and treatment. Cold Spring Harb Perspect Med 2:a007120PubMedCrossRef

Tan IL, Smith BR, von Geldern G, Mateen FJ, McArthur JC (2012) HIV-associated opportunistic infections of the CNS. Lancet Neurol 11:605–17PubMedCrossRef

van Marle G, Power C (2005) Human immunodeficiency virus type 1 genetic diversity in the nervous system: evolutionary epiphenomenon or disease determinant? J Neurovirol 11:107–28PubMedCrossRef

Wei F, Wang X, Liu L, Gao R, Shi Y, Zhang Y, Qiao L, Chen D (2011) Characterization of HIV type 1 env gene in cerebrospinal fluid and blood of infected Chinese patients. AIDS Res Hum Retroviruses 27:793–6PubMedCrossRef

Wu Z, Sun X, Sullivan SG, Detels R (2006) Public health. HIV testing in China Science 312:1475–6

Zhang Y, Qiao L, Ding W, Wei F, Zhao Q, Wang X, Shi Y, Li N, Smith D, Chen D (2012a) An initial screening for HIV-associated neurocognitive disorders of HIV-1 infected patients in China. J Neurovirol 18:120–6PubMedCrossRef

Zhang Y, Wang M, Li H, Zhang H, Shi Y, Wei F, Liu D, Liu K, Chen D (2012b) Accumulation of nuclear and mitochondrial DNA damage in the frontal cortex cells of patients with HIV-associated neurocognitive disorders. Brain Res 1458:1–11PubMedCrossRef

Titel: High levels of divergent HIV-1 quasispecies in patients with neurological opportunistic infections in China
verfasst von: Yulin Zhang
Feili Wei
Qi Liang
Wei Ding
Luxin Qiao
Fengli Song
Lifeng Liu
Sufang Yang
Ronghua Jin
Jianhua Gu
Ning Li
Dexi Chen
Publikationsdatum: 01.08.2013
Verlag: Springer US
Erschienen in: Journal of NeuroVirology / Ausgabe 4/2013
Print ISSN: 1355-0284
Elektronische ISSN: 1538-2443
DOI: https://doi.org/10.1007/s13365-013-0176-8

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