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01.11.2017 | Original Article

High thickness histological sections as alternative to study the three-dimensional microscopic human sub-cortical neuroanatomy

verfasst von: Eduardo Joaquim Lopes Alho, Ana Tereza Di Lorenzo Alho, Lea Grinberg, Edson Amaro Jr., Gláucia Aparecida Bento dos Santos, Rafael Emídio da Silva, Ricardo Caires Neves, Maryana Alegro, Daniel Boari Coelho, Manoel Jacobsen Teixeira, Erich Talamoni Fonoff, Helmut Heinsen

Erschienen in: Brain Structure and Function | Ausgabe 3/2018

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Abstract

Stereotaxy is based on the precise image-guided spatial localization of targets within the human brain. Even with the recent advances in MRI technology, histological examination renders different (and complementary) information of the nervous tissue. Although several maps have been selected as a basis for correlating imaging results with the anatomical locations of sub-cortical structures, technical limitations interfere in a point-to-point correlation between imaging and anatomy due to the lack of precise correction for post-mortem tissue deformations caused by tissue fixation and processing. We present an alternative method to parcellate human brain cytoarchitectural regions, minimizing deformations caused by post-mortem and tissue-processing artifacts and enhancing segmentation by means of modified high thickness histological techniques and registration with MRI of the same specimen and into MNI space (ICBM152). A three-dimensional (3D) histological atlas of the human thalamus, basal ganglia, and basal forebrain cholinergic system is displayed. Structure’s segmentations were performed in high-resolution dark-field and light-field microscopy. Bidimensional non-linear registration of the histological slices was followed by 3D registration with in situ MRI of the same subject. Manual and automated registration procedures were adopted and compared. To evaluate the quality of the registration procedures, Dice similarity coefficient and normalized weighted spectral distance were calculated and the results indicate good overlap between registered volumes and a small shape difference between them in both manual and automated registration methods. High thickness high-resolution histological slices in combination with registration to in situ MRI of the same subject provide an effective alternative method to study nuclear boundaries in the human brain, enhancing segmentation and demanding less resources and time for tissue processing than traditional methods.

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Titel: High thickness histological sections as alternative to study the three-dimensional microscopic human sub-cortical neuroanatomy
verfasst von: Eduardo Joaquim Lopes Alho
Ana Tereza Di Lorenzo Alho
Lea Grinberg
Edson Amaro Jr.
Gláucia Aparecida Bento dos Santos
Rafael Emídio da Silva
Ricardo Caires Neves
Maryana Alegro
Daniel Boari Coelho
Manoel Jacobsen Teixeira
Erich Talamoni Fonoff
Helmut Heinsen
Publikationsdatum: 01.11.2017
Verlag: Springer Berlin Heidelberg
Erschienen in: Brain Structure and Function / Ausgabe 3/2018
Print ISSN: 1863-2653
Elektronische ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-017-1548-2

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High thickness histological sections as alternative to study the three-dimensional microscopic human sub-cortical neuroanatomy

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