Introduction
Percentage of positive prostate biopsy cores is a known independent risk factor for PCa aggressiveness. This was demonstrated nearly two decades ago when D’Amico et al. revealed PCa biopsy core involvement of ≥ 50% was associated with a 10.4 × relative risk of prostate-specific mortality versus those with < 50% biopsy core involvement [
1]. This pathologic factor was ultimately used to help differentiate more belligerent forms of intermediate-risk PCa [
2]. Consequently, it is currently included within the NCCN guidelines as an independent factor for the diagnosis of unfavorable intermediate-risk PCa.
The majority of data exploring the association between prostate biopsy core involvement and radiotherapy outcomes was published in the era of conventional fractionation. In the modern era, ablative radiotherapy techniques (i.e. SBRT) have demonstrated comparative effectiveness relative to conventional fractionation [
3,
4]. Much of the early interest in ultra-hypofractionated schedules in the treatment of PCa grew out of our understanding that prostatic adenocarcinoma has a far lower alpha/beta (~ 1.85) than previously hypothesized [
5]. As such, SBRT of 40 Gy in five fractions can deliver a much higher biologically effective dose (BED) compared to dose escalated intensity modulated radiation therapy (IMRT) of 78 Gy in 39 fractions (213 vs. 162, respectively).
It may be postulated, a larger BED could lead to volumetric eradication of adenocarcinoma to a greater extent. A recent publication from Memorial Sloan Kettering demonstrated two year post-SBRT prostate biopsies yielded lower rates of positivity when SBRT doses ≥ 40 Gy in five fractions were utilized [
6]. Moreover, those patients found to have a positive two-year post-SBRT biopsy were significantly more likely to develop a biochemical relapse at five years. As such, there appears to be a correlation between dose fractionation and pathological ablation resulting in long-term biochemical control.
In the era of SBRT, can a higher BED mitigate the historical negative prognostic implications of high-volume disease? Herein, we explore the association between the extent of pre-SBRT prostate biopsy core involvement with post-treatment PSA kinetics and oncologic outcomes.
Materials and methods
Patient eligibility and treatment
The local Institutional Review Board (Study # 00001269) approved this single institutional review of patients treated for PCa. Exclusion criteria for the specific investigation of PSA nadir outcomes was as follows: (1) received ADT as a component of treatment, (2) did not have a pathology report available, (3) never achieved a PSA nadir (i.e. PSA < 3 ng/mL), (4) demonstrated disease progression, and (5) less than 2 years of FU. Analysis of core involvement association with oncologic outcomes excluded (3) and (4) above. All patients underwent rigorous surgical pathologic review of the prostate biopsy specimen. Each prostate pathology report was independently reviewed by our team to document the number of cores that were sampled, location, and the Gleason score that was identified. This evaluation included adenocarcinoma core involvement, overall percentage core involvement, and specific GS for a given positive core (PC). All patients were evaluated by a radiation oncologist and deemed appropriate for definitive 5-fraction SBRT. All patients underwent computed tomography (CT)-based radiation treatment planning simulation. A prostate MRI was obtained in the majority of cases at the time of simulation. Patients underwent robotic SBRT with a clinical target volume (CTV) which included the entire prostate and proximal seminal vesicles. A 5 mm isometric expansion of the CTV with a tighter 3 mm posterior margin was used to create the PTV.
Follow-up and statistical analysis
Patients were typically followed using serial PSA and clinical examination at 3-month intervals for the first year and subsequently every 6 to 12 months thereafter. PSA nadir was defined as the lowest post-SBRT PSA obtained after at least 2 years of FU. High-volume core involvement was defined in accordance with the NCCN definition of ≥ 50% biopsy core involvement with adenocarcinoma. Descriptive statistics (mean ± standard deviation or median [25th, 75th] percentiles for continuous variables; frequency and percent for categorical variables) were calculated for the overall sample for patient, tumor, and treatment characteristics. A graphical display of PSA nadir was constructed using boxplots for the overall sample as well as stratified by low-, intermediate-, and high-risk groups.
The association between PSA nadir and percentage of PC, continuous percentage of core involvement and age, and PSA and prostate CTV was assessed using Spearman correlation coefficients. Core involvement was then analyzed by giving higher weight to PC involvement of higher-grade grouping. For example, a single core of GS 10 was weighted 5 × a single core involved of GS 6. Grade group weighting was as follows: GS6 – 1x, GS7 – 2x, GS8 – 3x, GS9 – 4x, GS10 – 5×. Analysis of variance was used to assess the association between categorical variables such as PSA, GS, NCCN Risk and continuous percent core involvement. Percent core involvement was dichotomized as < 50% and ≥ 50%. The two groups were compared using the chi-square test or Fisher’s exact test for categorical variables, and the two-sample t-test or Mann–Whitney test for continuous variables.
Time-to-failure analysis (biochemical and/or radiological failures) was accomplished using standard methods of survival analysis, where the data were stratified by dichotomized percent PC (< 50% vs. ≥ 50%). Biochemical failure was defined using the Phoenix definition of > 2 ng/mL above nadir. In cases where the endpoint event, “failure”, had not yet occurred, the number of years until last FU was used and ‘censored’. The groups were compared using the log-rank test. Cox Proportional Hazards regression was used to determine whether weighted total PCs were associated with “time-to-failure” alone, and after adjusting for the possible confounding effect of prostate CTV. All analyses were performed for the overall sample, and separately by NCCN risk group. A result was considered statistically significant at p < 0.05. Analyses were performed using SAS version 9.4 (SAS institute Inc.,). A multivariable analysis was conducted controlling for age, initial PSA, GS, staging and prostate CTV.
Discussion
In the present study with a median FU of over 4 years, we demonstrate no increased risk of bPFS in patients with high-volume core involvement who were treated for localized PCa with 5-fraction SBRT monotherapy. In fact, even when weighting biopsy core involvement by aggressiveness of histology, there was no correlation with increased risk of bPFS for intermediate risk disease. In general, high-volume core involvement was a relatively rare event, at least in a group of patients who did not receive ADT, occurring in 19% of our cohort. Moreover, high-volume core involvement was more commonly observed in patients who had intermediate-risk disease or smaller PV. Counterintuitively, within the intermediate cohort, PSA nadirs were found to be significantly higher in patients with low-volume core involvement. This may reflect PSA expression resilience in those with a higher volume of normal prostatic tissue.
Biopsy core involvement is inextricably linked to the type of biopsy performed and the volume of the prostate being biopsied [
7,
8]. In a vacuum, a 12-core biopsy performed on a 20 cc prostate is naturally more reflective of the true cancer distribution versus a 12-core biopsy performed on a 200 cc prostate, as has been reflected in the literature [
9]. This was manifested in our analysis, as high-volume core involvement was associated with smaller CTV. Prior research has demonstrated more extensive prostate biopsies (21 vs. 12 cores) correlate with a lower rate of identifying surprising unfavorable disease at prostatectomy [
10]. The geometry of core involvement also plays a role, with contiguous biopsy core involvement having been demonstrated to correlate with extracapsular extension and seminal vesicle invasion [
11]. As MRI-targeted biopsies become ubiquitous, the utility of capturing disease volume based on a 12-core blind biopsy is challenging to ascertain. Even the correct denominator used in calculating percentage core involvement when multiple targeted biopsies of a given ROI is calculated variably.
Prostate biopsy tumor quantitation has been explored using a variety of metrics in the literature including percent PC involvement, as in the present study, as well as greatest percentage of the most involved core and highest cumulative core length, amongst other metrics. Murgic et al. demonstrated the maximum involvement of a given biopsy core is the only prognostic factor for freedom from biochemical failure following conventionally fractionated radiation [
12]. Percentage of positive prostate biopsy cores has been shown to be predictive on MVA of PSA outcome following surgical prostatectomy [
13]. Finally, a review of 13 manuscripts determined prostate tumor quantitation using overall percentage or the greatest percentage of the most involved core was associated with clinical outcomes [
14].
Within the low-risk realm, percentage core involvement makes a large impact in the decision making for those patients evaluated for active surveillance candidacy. The number of PC involved has been demonstrated as an independent risk factor on MVA for progression after first biopsy [
15]. In general, core involvement appears to be predictive for upstaging low-risk cancers at the time of prostatectomy [
16,
17]. Though, data regarding the value of prostate biopsy volume characteristics in low-risk PCa after treatment is conflicting with some reports highlighting its importance and others its insignificance [
18‐
20].
Within the high-risk realm, increasing number of PC with high-grade cancer and > 50% PC involvement are predictive for unfavorable pathology identified at radical prostatectomy [
21]. Maximum volume of high-grade (GS8-10) cancer per core has been shown on MVA as an independent predictor of final GS at pT stage [
22]. Greatest percentage of a given involved biopsy core length has also been associated with adverse clinical outcomes following prostatectomy [
23]. Higher cumulative PCa core length relative to the number of biopsy cores sampled is associated with identification of higher volume PCa at the time of prostatectomy, though it is unclear if this is confounded by PV [
24].
A major limitation of the present study is the lack of differentiation of GS7 cancers into the more modern GG 2 versus 3 disease, which is a remnant of the retrospective evaluation of our pathology database. For the purposes of PSA nadir analysis, ADT was exclusionary and as such, we limit the generalizability of this dataset to only those patients who did not receive ADT in concert with SBRT. It is reasonable to hypothesize that high-volume core involvement would more commonly trigger ADT inclusion, and thus these patients were excluded from the present analysis. In addition, although the pathology reports were predominately based off 12 core prostate biopsies, some did include MRI-targeted biopsies. In such cases, a given region of interest with multiple biopsies taken was not counted as a single biopsy, as is now recommended in the modern version of the NCCN. The use of CTV as a surrogate for PV is also subject to error, particularly if large-volume seminal vesicles were included. Finally, the analysis of core involvement is also subject to selection bias given all patients received SBRT, and patients managed with other modalities of treatment were excluded.
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