Introduction
Materials and methods
Patients and data collection
Patient_id | Sample_id | Age | Stage | Diagnosis |
---|---|---|---|---|
P1 | T1, ASC1, M1 | 57 | IIIc | HGSOC |
P2 | T2, ASC2, M2 | 64 | IIIc | HGSOC |
P3 | T3, ASC3, M3 | 71 | IIIc | HGSOC |
P4 | T4, ASC4, M4 | 78 | IIIc | HGSOC |
P5 | T5, M5 | 67 | IIIc | HGSOC |
P6 | T6, M6 | 62 | Ia | HGSOC |
P7 | T7, M7 | 62 | IIIc | HGSOC |
P8 | T8, M8 | 77 | IIIc | HGSOC |
P9 | T9, M9 | 63 | III | HGSOC |
P10 | T, ASC, M | 63 | IV | HGSOC |
P11 | T, ASC, M | 55 | IV | HGSOC |
P12 | T, ASC, M | 64 | IIIc | HGSOC |
P13 | T, ASC, M | 48 | IIIa | HGSOC |
P14 | T, ASC, M | 59 | IV | HGSOC |
P15 | T, ASC, M | 77 | IV | HGSOC |
P16 | T, ASC, M | 77 | IIIc | HGSOC |
P17 | T, ASC, M | 52 | IIIc | HGSOC |
Cell lines and cell culture
Total RNA extraction and quantitative real-time PCR
Western blot assay
Plasmid construction and transfection
Wound healing assay
Transwell migration and invasion assays
EdU assay
RNA-sequencing assay
Single sample gene set enrichment (ssGSEA) analysis
Mfuzz soft clustering analysis
Prediction of miRNA binding sites and target genes
Statistical analysis
Results
Transcriptomics profiling identified EMT-MET in EOC via the peritoneal circulation
Screening for circRNAs differentially expressed in ascitic metastasis of ovarian cancer
CircRNA_Id | Position | Strand | Spliced length | Best transcript | Gene symbol |
---|---|---|---|---|---|
hsa_circ_0031584 | chr14:32559707–32586493 | + | 4033 | NM_001030055 | ARHGAP5 |
hsa_circ_0002925 | chr9:115336336–115337531 | + | 1195 | NM_133465 | KIAA1958 |
hsa_circ_0005325 | chr19:34921480–34925873 | + | 321 | NM_005499 | UBA2 |
hsa_circ_0000048 | chr1:33413822–33415375 | − | 351 | NM_153341 | RNF19B |
hsa_circ_0000137 | chr1:155408117–155408859 | − | 742 | NM_018489 | ASH1L |
hsa_circ_0081207 | chr7:97820925–97823884 | + | 2959 | NM_014916 | LMTK2 |
hsa_circ_0000497 | chr13:78293666–78327493 | + | 788 | NM_001040153 | SLAIN1 |
hsa_circ_0000918 | chr19:19750893–19751380 | − | 299 | NM_016573 | GMIP |
hsa_circ_0006618 | chr4:83799882–83803093 | − | 406 | NM_001077207 | GMDS-AS1 |
hsa-GMDS-AS1_0002 | chr6:2263601–2273417 | + | 666(estimated) | GMDS-AS1 |