Managed by Antonio Secchi.
The online version of this article (doi:10.1007/s00592-016-0943-0) contains supplementary material, which is available to authorized users.
Zhenzhou Zhao and Xuejie Li have contributed equally to this work.
The purpose of the current study was to investigate the characteristic expression of circular RNAs (circRNAs) in the peripheral blood of type 2 diabetes mellitus (T2DM) patients and their potential as diagnostic biomarkers for pre-diabetes and T2DM.
CircRNAs in the peripheral blood from six healthy individuals and six T2DM patients were collected for microarray analysis, and an independent cohort study consisting of 20 normal cases, 20 pre-diabetes patients and 20 T2DM patients was conducted to verify the five chosen circRNAs. We then tested hsa_circ_0054633 in a third cohort (control group, n = 60; pre-diabetes group, n = 63; and T2DM group, n = 64) by quantitative real-time polymerase chain reaction (Q-PCR).
In total, 489 circRNAs were discovered to be differentially expressed between the two groups, and of these, 78 were upregulated and 411 were downregulated in the T2DM group. Five circRNAs were then selected as candidate biomarkers and further verified in a second cohort. Hsa_circ_0054633 was found to have the largest area under the curve (AUC). The diagnostic capacity of hsa_circ_0054633 was tested in a third cohort. After introducing the risk factors of T2DM, the hsa_circ_0054633 AUCs for the diagnosis of pre-diabetes and T2DM slightly increased from 0.751 (95% confidence interval [0.666–0.835], P < 0.001) to 0.841 ([0.773–0.910], P < 0.001) and from 0.793 ([0.716–0.871], P < 0.001) to 0.834 ([0.762–0.905], P < 0.001), respectively.
Hsa_circ_0054633 presented a certain diagnostic capability for pre-diabetes and T2DM.
Rahelic D (2016) 7th edition of idf diabetes ATLAS–CALL for immediate action. Lijec Vjesn 138:57–58 PubMed
Ghasemi A, Tohidi M, Derakhshan A, Hasheminia M, Azizi F et al (2015) Cut-off points of homeostasis model assessment of insulin resistance, beta-cell function, and fasting serum insulin to identify future type 2 diabetes: Tehran Lipid and Glucose Study. Acta Diabetol 52:905–915. doi: 10.1007/s00592-015-0730-3 CrossRefPubMed
Alberti KG, Zimmet PZ (1998) Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 15:539–553. doi: 10.1002/(sici)1096-9136(199807)15:7<539:aid-dia668>3.0.co;2-s CrossRefPubMed
Gatling W, Begley J (2001) Diagnosing diabetes mellitus in clinical practice: is fasting plasma glucose a good initial test? Pract Diabetes Int 18:89–93 CrossRef
Moran I, Akerman I, van de Bunt M, Xie R, Benazra M et al (2012) Human beta cell transcriptome analysis uncovers lncRNAs that are tissue-specific, dynamically regulated, and abnormally expressed in type 2 diabetes. Cell Metab 16:435–448. doi: 10.1016/j.cmet.2012.08.010 CrossRefPubMedPubMedCentral
- Hsa_circ_0054633 in peripheral blood can be used as a diagnostic biomarker of pre-diabetes and type 2 diabetes mellitus
- Springer Milan
Neu im Fachgebiet Innere Medizin
Meistgelesene Bücher aus der Inneren Medizin
Mail Icon II