Introduction
Materials and methods
Study population
Study process
The definitions of pre-diabetes and T2DM and collection of whole blood samples
RNA extraction and Q-PCR
CircRNA microarray analysis
Data analysis
Results
Expression profiles of circRNAs in the peripheral blood of diabetic patients
The expression profile of circRNAs verified by Q-PCR
ROC curve analysis of circRNAs with differential expression
CircRNA | AUC | 95% CI |
P value | Sensitivity | Specificity | Fold change |
---|---|---|---|---|---|---|
hsa_circ_0054633 | ||||||
Pre-diabetes | 0.747 | 0.589–0.906 | 0.007 | 0.75 | 0.70 | 1.7 |
T2DM | 0.720 | 0.562–0.878 | 0.017 | 0.55 | 0.85 | 1.8 |
hsa_circ_0068087 | ||||||
Pre-diabetes | 0.692 | 0.529–0.856 | 0.037 | 0.80 | 0.50 | 1.8 |
T2DM | 0.717 | 0.557–0.878 | 0.019 | 0.90 | 0.50 | 1.6 |
Further clinical validation of the biomarker
Biomarker expression levels in different gender and age groups
Variable | Number | Control group | Pre-diabetes group | T2DM group | |||||
---|---|---|---|---|---|---|---|---|---|
Expression quantity Median (quartile) |
P value | Number | Expression quantity Median (quartile) |
P value | Number | Expression quantity Median (quartile) |
P value | ||
Age (years) | |||||||||
<50 | 37 | 0.116 (0.072,0.148) | 0.665 | 34 | 0.199 (0.128,0.277) | 0.363 | 32 | 0.414 (0.235,0.496) | 0.301 |
≥50 | 23 | 0.102 (0.073,0.199) | 29 | 0.155 (0.120,0.259) | 32 | 0.350 (0.246,0.450) | |||
Gender | |||||||||
Male | 29 | 0.099 (0.066,0.169) | 0.762 | 32 | 0.183 (0.126,0.261) | 0.45 | 35 | 0.393 (0.256,0.558) | 0.438 |
Female | 31 | 0.133 (0.074,0.154) | 31 | 0.170 (0.129,0.375) | 29 | 0.364 (0.232,0.483) |