Humoral immunoreactivity to gliadin and to tissue transglutaminase is present in some patients with multiple myeloma

Multiple myeloma (MM) is a clonal B-cell disorder which diagnosis comprise the examination of bone marrow for plasma cell infiltration, detection and quantification of monoclonal protein "M" component in the serum or urine, and evidence of end-organ damage (hypercalcemia, renal insufficiency, anemia or bone lesions). Many of the laboratory parameters contribute to myeloma diagnosis due to plenty of immunological disturbances [1]. It was shown that the antibodies contained in M component have various specificity to: some proteins, double-stranded DNA, several antibiotics [2], and sometimes to gliadin (and/or calreticulin?) [3].

As the enhanced levels of the serum antibodies to gliadin are found in patients with celiac disease, as well as of antibodies to transglutaminase-2 (TTG-2) [4, 5], to calreticulin [6, 7] and Ro/SSA antigen [8], the aim of this work was the screening of MM patients' sera for their immunoreactivity to food constituent gliadin, and to autoantigens: tissue transglutaminase-2 (tTG-2) and Ro/SSA antigen, in order to assess whether immunoreactivity to mentioned antigens at least partially contributes to the immunological imbalance in multiple myeloma.

Cut off values of anti-gliadin reactivity obtained analyzing 50 healthy sera were 3.46 IU/ml for IgA and 5.83 IU/ml for IgG.

The elevated serum IgA immunoreactivity to gliadin was found in 14/56 patients and in one of controls. From these patients, 4 were with IgA myeloma and 4 were with IgG myeloma, while 6 were without M component in their sera.

Statistical analysis of data obtained revealed that the level of anti-gliadin IgA immunoreactivity for patients with MM was not significantly differ than that for controls (P = 0.052).

Surprisingly, the elevated IgG immunoreactivity to gliadin was found only in two of tested MM patients sera and in two of control people (Fig 1.) and both patients were with IgG myeloma.

Cut off values of anti-tTg reactivity obtained analyzing 50 healthy sera were 1.86 IU/ml for IgA and 6.17 IU/ml for IgG.

As seen on Fig. 2.a. higher than cut off of IgA immunoreactivity to tTG was found in 10/49 patients and 2 of controls. From 10 anti-tTg IgA positive patients 4 were with IgA and 4 were with IgG myeloma, while 2 were without M component in their sera. Statistical analysis of data obtained revealed that the level of anti-tTG-2 IgA immunoreactivity in patients with myeloma was significantly higher than that obtained in healthy controls (P < 0.02).

Sera from 4/45 patients and 3 controls had higher than cut off value for IgG immunoreactivity (Fig. 2.b). All 4 anti-tTg IgG positive patients were with IgG MM.

Cut off values of anti-Ro/SSA IgG reactivity obtained analyzing 50 healthy sera was 3.59 IU/ml. The enhanced IgG immunoreactivity to RoSSÀ antigen found in this work in 9/47 analyzed MM patients' sera (Fig. 3). These anti-RoSSÀ IgG positive patients were: 2 with IgA, 5 with IgG, and 2 without M component.

In this work, the immunoreactivity to one of the food constituents is found in some MM patients. It is manifested through the enhanced levels of serum IgA immunoreactivity to gliadin. Data obtained by ELISA tests indicate that especially anti-gliadin, IgA immunity is developed in patients with MM. It seems that the elevated immunoreactivities to gliadin could not be the consequence of overproduction of immmunoglobulins from M component, because the enhanced antigliadin IgA immunoreactivity was found in the serum of some of MM patients without M component as well as in patients with IgG myeloma too. It is also possible that in some patients serum antigliadin immunoreactivities could be hidden in immune complexes in circulation (CIC) and in these cases this reactivity could not be revealed by ELISA test. One of the solutions for this problem could be the electrophoresis of serum proteins performed in the alkaline conditions pH = 8.6 i.e., in conditions which enables dissociation of antibodies from antigen in CIC [9, 10]; these antibodies physically separated from the gliadin by electrophoresis could then again react with gliadin (0.2%, or 0.4% in 0.5% SDS) in the test for immunofixation [3].

It must be mentioned that only the level of anti tTG-2 IgA immunoreactivity in patients with myeloma was significantly higher than these obtained in healthy controls. Besides, our results show that autoimmune determinants as the enhanced immunity to tTG-2, and to Ro/SSA antigen, which are present in patients with gluten intolerance [4, 5] and autoimmune diseases [8] respectively, are present in some patients with MM, too.

Immunoreactivity to some of these antigens was also found in few of healthy controls, indicating that the immunoreactivities to tTG or to Ro/SSA antigens, or to gliadin are not strictly specific for MM, but that they are only one part of the complex immunological disturbances found in this disease. Findings from this work are in accordance with the previously published reports [11‐14] pointing to the partial immunological similarity between MM and celiac disease.

This work indicates that gliadin as food antigen could at least partially contribute to the plethora of immunological disturbances in some patients with MM. Due to the aminoacid sequence equality or high homology between α-gliadin and γ-gliadin and C albicans–hyphal wall protein 1 (HWP1), and with calreticulin, it is also possible that the determined immunoreactivity with gliadin is in part the consequence of the cross reactivity with mentioned proteins [15].

Data from this work showed the existence of the enhanced serum immunoreactivity to gliadin, tTG-2 and Ro/SSA antigens in some patients with MM and set up the question whether MM is also on the list of malignant diseases which are associated with immunological aspect of gluten intolerance [16‐19]. They call for the additional research to elucidate the clinical importance of both: the elevated humoral immunity to food antigens and to some autoantigens in patients with MM.