Background
Intuitive understanding of hypothetical genes’s signal peptide, transmembrane helix (TH) domain and LipoP box of any pathogenic microorganisms is a prerequisite for the rational development of drugs and vaccine candidates. A protein harboring these sequences may serve as receptor and conveyer for various biostimulations generated during host-pathogen interaction. Identification and annotation of these proteins may help in designing of novel drug or developing new vaccine candidates to control CT infections.
Methods
We have retrieved whole sequence of CT serovar D/UW-3/CX from NCBI database. To identify secreted proteins, TH domain and lipoP box, we have exploited the following online softwares: sigpred (http://bmbpcu36.leeds.ac.uk/cgibin/sig_pred), siglanlP4.0 (http://www.cbs.dtu.dk/services/SignalP), TMpred (http://www.ch.embnet.org/software/TMPRED_form.html), TMHMM (http://www.cbs.dtu.dk/services/TMHMM/) and LipoP box (http://www.cbs.dtu.dk/services/LipoP/).
Anzeige
Results
The present study focused on the 336 HPs of CT to find out signal peptide, TH domain and lipoP box. Out of 336 HPs. Forty proteins have lipoP box and five proteins (CT149, CT605, CT623, CT823 and CT858) have signal peptide as well as TH domain. Additionally, among five, four proteins (CT149, CT623, CT823 and CT858) also have lipoP box. Harboring TH domain sequences suggests that they may have either membrane spanning, extracellular or intracellular domain which may help in host-pathogen interactions and/or signal transduction. It is also possible that these proteins may be secreted and thereby induces host immune responses but it will need further validation.
Conclusion
We consider that CT149, CT605, CT623, CT823 and CT858 may be secreted outside the pathogen and should be explored further to understand the pathogenesis of CT.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.