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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Reproductive Biology and Endocrinology 1/2012

Immature rat seminal vesicles show histomorphological and ultrastructural alterations following treatment with kisspeptin-10

Zeitschrift:
Reproductive Biology and Endocrinology > Ausgabe 1/2012
Autoren:
Faiqah Ramzan, Irfan Zia Qureshi, Muhammad Ramzan, Muhammad Haris Ramzan, Faiza Ramzan
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1477-7827-10-18) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

FR has conducted the entire work, designed the study and drafted the manuscript. IZQ has supervised the study. MR and MHR has contributed the data analysis. FR has contributed to histology. All authors read and authorized the final manuscript.

Abstract

Background

Degenerative effects of critical regulators of reproduction, the kisspeptin peptides, on cellular aspects of sexually immature male gonads are known but similar information on accessory sex glands remain elusive.

Methods

Prepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day. Control rats were maintained in parallel. The day following the end of the experimental period, seminal vesicles were removed and processed for light and electron microscopic examination using the standard methods. DNA damage was estimated by DNA ladder assay and DNA fragmentation assay.

Results

The results demonstrated cellular degeneration. Epithelial cell height of seminal vesicles decreased significantly at all doses (P < 0.05). Marked decrease in epithelial folds was readily noticeable, while the lumen was dilated. Ultrastructural changes were characterized by dilatation of endoplasmic reticulum and Golgi complex, heterochromatization of nuclei, invagination of nuclear membranes and a decreased number of secretory granules. Percent DNA damage to the seminal vesicle was 19.54 +/- 1.98, 38.06 +/- 2.09 and 58.18 +/- 2.59 at 10 pg, 1 ng and 1 microgram doses respectively.

Conclusion

The study reveals that continuous administration of kisspeptin does not lead to an early maturation but instead severe degeneration of sexually immature seminal vesicles.
Zusatzmaterial
Authors’ original file for figure 1
12958_2011_966_MOESM1_ESM.pdf
Authors’ original file for figure 2
12958_2011_966_MOESM2_ESM.jpeg
Authors’ original file for figure 3
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Authors’ original file for figure 4
12958_2011_966_MOESM4_ESM.pdf
Literatur
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