Introduction
In adult patients both sporadic inclusion body myositis (s-IBM) and hereditary inclusion body myopathy (h-IBM) are classified as progressive muscular diseases [
1,
2]. They are characterized by similar pathological features, including abnormal inclusions and vacuoles in myofibers, however, h-IBM shows no signs of inflammation in the muscle biopsy [
3,
4].
Sporadic IBM belongs to a group of idiopathic inflammatory myopathies of unknown cause with inflammatory and degenerative features resulting from autoimmunity [
5‐
9]. However, many scientists feel that it is a primary degenerative disorder that triggers inflammation [
10,
11]. It is a progressive disease and is the most common disease of skeletal muscle in patients over the age of 50 [
6,
12], leading to serious disability [
3,
7,
13]. The onset of symptoms in patients younger than 60 years of age is typical for 18–20% of patients [
6]. The occurrence of IBM varies between 4.3 and 9.3 per a million births, with an increase to 35.3 among people over 50 years of age [
5]. Clinically this disease entity is characterized by slow and progressive weakening of distal muscles of the arms (emaciated forearms) and proximal muscles of the legs. A biopsy reveals inflammatory cell infiltrates in the endomysium, vacuoles with granularities, the so-called rimmed vacuoles (RVs), as well as necrosis of muscle fibers and deposits of abnormal proteins [
2,
3,
6,
7,
9,
14,
15]. Many authors distinguish four basic subgroups of idiopathic inflammatory myopathies: dermatomyositis (DM), polymyositis (PM), immune-mediated necrotizing myopathy (NM) and IBM [
6,
7,
15,
16]. However, from a clinical standpoint s-IBM, is distinguishable from the aforementioned diseases by asymmetric weakness of the finger flexor and knee extensor muscles, atrophy of the quadriceps muscles of the thighs with an inability to fully straighten the knees and resistance to immunosuppressive therapy [
3,
6,
7,
17]. It should be emphasized that patients demonstrating clinical symptoms typical for IBM with few inflammatory cells or with the presence of RVs, as seen in a muscle biopsy examination, could be difficult to diagnose in terms of histopathology [
6]. Moreover, patients suffering from PM may have several RVs [
6]. Although RVs are a hallmark of IBM, they can also be found in 30 other muscular or neurogenic disorders, e.g., in spinal muscular atrophy or muscular dystrophies [
18,
19]. Therefore, morphological determinants of s-IBM are not specific and appear in various neuromuscular disorders [
20]. Similarly, electromyography (EMG) examination makes it possible to include this disease in a group of muscle damage of the myogenic type, without the possibility of precisely determining its cause [
21]. Hence, the initial diagnosis is often incorrect, and it is likely to be assessed as PM or as amyotrophic lateral sclerosis (ALS) [
7,
9].
The most reliable way of identifying this disease is a muscle biopsy followed by histopathological and immunohistochemical assessment [
5,
14]. Immunohistochemistry (IHC) is a method which enables the detection of various proteins that accumulate in muscle fibers of patients suffering from s-IBM and that are associated with inflammation processes, autophagy or endoplasmic reticulum stress [
7,
17,
22]. Accumulated proteins have the immunoreactivity of beta-amyloid, Tau protein and apolipoproteins [
20,
23‐
25]. For the final diagnosis of this disease it is necessary to carry out research with a transmission electron microscope (TEM), in order to demonstrate the presence of RVs and disorganization of myofibrils [
7,
23,
26,
27].
The aim of this study was to identify the distribution of specific antigens as well as to determine morphological features in the skeletal muscles of patients diagnosed with s-IBM.
It is important to emphasize that the very slow progress of clinical symptoms makes the diagnosis difficult. Another problem was a study in transmission electron microscope (TEM) which is currently pivotal in the diagnostic scheme. Not much research has focused exclusively on the pathologically defined IBM (according to modified IBM diagnostic criteria proposed by Hilton-Jones et al. [
28]). For this reason, we would like to emphasize the morphological aspect during the diagnosis of this disease and underline the role of IHC reaction and transmission electron microscopy that are crucial for pathologically defined IBM. Therefore, we have tried to accentuate the practical aspects of our study which include unique muscle images resulting from an innovative combination of IHC and TEM techniques. To the best of our knowledge there is no literature in this area of research.
Materials and methods
Patients
All patients were hospitalized and treated between 2011 and 2016 in the Department of Rheumatology and Internal Medicine of the Wroclaw Medical University. They were between 48 and 83 years old and on the average had felt symptoms for the previous 5 years. The research material was constituted of small skin samples (13 mm wide and 4 mm deep) with fragments of subcutaneous tissue and skeletal muscles (12 mm wide and 4 mm deep) of the lower limbs: quadriceps femoris, rectus femoris, gastrocnemius and of the upper limbs: biceps brachii and deltoid muscle collected from six patients diagnosed with s-IBM.
All patients signed an informed consent for muscle biopsy and histopathological study, based on local regulations and approved by the Wroclaw Medical Hospital (part of Wroclaw Medical University). Diagnostic criteria for patients are concomitant with definite IBM according to Griggs et al. [
29] and Dalakas [
5] (without tubulofilaments in TEM), together with pathologically defined IBM after the modified criteria of Hilton-Jones et al. [
28]. In our study, we could definitely exclude possible IBM by using anti-beta-amyloid antibody which detected the presence of amyloid deposits. Exclusion criteria were as follows: toxic and drug-induced (including statins, glucocorticoids) myopathies, rhabdomyolysis, and injury; polymyositis was eliminated on the basis of clinical features and histopathological examination. Clinical characteristics of patients are given in Table
1.
Table 1
Clinical characteristics of six patients with sporadic inclusion body myositis (s-IBM) and applied treatment [
8]
1 | Female | 48 | 10 | Biceps brachii | Primary muscle damage | 413 | 1441 | MTX, GKS, MMF |
2 | Female | 54a | 1 | Biceps brachii, gastrocnemius | Primary muscle damage | 402 | 1563 | MTX, GKS |
3 | Female | 61 | 4 | Biceps brachii | Motor polyneuropathy | 402 | 2501 | MMF |
4 | Male | 70 | 5 | Gastrocnemius | Primary muscle damage | 337 | 521 | RTX, AZA, GKS, MMF |
5 | Female | 81 | 1 | Deltoid muscle | Motor polyneuropathy | 265 | 160 | MTX, GKS, AZA, MMF |
6 | Male | 83 | 4 | Quadriceps femoris, rectus femoris | Primary muscle damage | 246 | 224 | MMF |
Light microscopy
Tissue samples were embedded in paraffin blocks (Merck, Darmstadt, Germany) which were cut on fully automated Rotary Microtome (Leica RM 2255, Nussloch, Germany) into 4-µm-thick paraffin sections. Subsequently, sections were stained with Mayer’s hematoxylin and eosin (H&E) (Bio-Optica Milano, Italy) and were analyzed with the use of a light microscope BX41 (Olympus, Tokyo, Japan), connected to a digital camera Colorview IIIu (Olympus).
Immunohistochemistry (IHC)
Immunohistochemical reactions were performed on 4-µm-thick paraffin sections using Autostainer Link48 (Dako, Glostrup, Denmark) and the following antibodies: anti-beta-amyloid (Dako), anti-Tau protein (Invitrogen, Carlsbad, USA), anti-apolipoprotein B (apo-B; Santa Cruz Biotechnology, Dallas, USA), anti-CD3 (T lymphocytes marker; Dako), anti-CD8 (cytotoxic T lymphocytes marker; Dako), anti-CD68 (macrophages marker; Dako) and anti-cN1A (anti-NT5C1A; Abcam). Briefly, deparaffinization, rehydration and the antigens unmasking were performed with EnVision FLEX Target Retrieval Solution (pH 9, 20 min, 97 °C; Dako) using PTLink platform (Dako). The activity of endogenous peroxidase was blocked by 5-min incubation with EnVision FLEX Peroxidase-Blocking Reagent (Dako). Afterwards, primary antibodies directed against beta-amyloid (1:300), Tau protein (1:3000), apolipoprotein B (1:100), cN1A (1:200), CD3 (RTU), CD8 (RTU), and CD68 (RTU) were applied for 20 min. For visualization EnVision FLEX/HRP (Dako, 20 min) was used. As a peroxidase substrate 3,3′-diaminobenzidine (DAB; Dako) was used for 10 min of incubation. Finally, all sections were counterstained with EnVision FLEX Hematoxylin (Dako) for 5 min. After dehydration in gradually increasing ethanol concentrations (70%, 96% and 99.8%), followed by incubation in xylene, slides were closed with coverslips in Dako Mounting Medium (Dako). The primary antibodies were diluted in EnVision FLEX Antibody Diluent (Dako).
Transmission electron microscope (TEM)
For TEM examination skeletal muscle samples from six patients were fixed in 2.5% glutaraldehyde (Serva Electrophoresis, Heidelberg, Germany). After 24 h, the specimens were washed in cacodylate buffer (0.1 M, pH 7.4, Serva) and postfixed for 1 h in 1% osmium tetroxide (Serva). Subsequently, they were rinsed with the cacodylate buffer. Afterwards, the specimens were dehydrated in highly concentrated ethyl alcohol and twice in pure acetone (Chempur, Piekary Slaskie, Poland). Samples were embedded in epoxy resin (Epon 812, Serva). To select the examined area for TEM Epon blocks were cut into semithin, 600-nm-thick sections, stained with toluidine blue (Serva) and closed with the use of Euparal mounting agent (Roth, Mannheim, Germany). Finally, ultrathin, 50-nm-thick sections were prepared using an ultramicrotome Power Tome XL (RMC, Tucson, USA). To improve the contrast, ultrathin sections placed on the copper grids were counterstained with uranyl acetate and lead citrate (Serva), and examined in the TEM JEM-1011 (JEOL, Tokyo, Japan). Digital micrographs were prepared with the use of TEM imaging platform iTEM1233 equipped with a Morada Camera (Olympus, Münster, Germany) at magnifications ranging from 5 to 20 K.
Discussion
Early clinical symptoms which should raise suspicion of s-IBM include difficulty in climbing stairs and rising from a sedentary position, aching of thighs and knees and difficulties in using different hand tools [
30]. In addition, weakness of the thigh’s quadriceps muscles, increased serum CK level, the presence of RVs and atrophic myofibers in muscle biopsies could help to diagnose patients suffering from s-IBM [
9,
31]. There are some reports showing that physical effort may improve skeletal muscle strength and function, which would encourage its implementation immediately after s-IBM has been diagnosed [
6,
32]. Since s-IBM is an untreatable disease establishing the final diagnosis is critical for patients in order to avoid an ineffective immunosuppressive therapy with its many side effects [
9,
15], and to start physiotherapy [
33].
In our study the clinical features, including weakness of upper and lower extremities, difficulty in climbing stairs as well as patients’ ages were typical and similar to other reports [
12,
27,
34]. Moreover, similarly to Hermanns et al. [
4] and Sakai et al. [
17] we observed periodical increases in the levels of CK and LDH enzymes in the studied patients’ serum. In patients from a study conducted in China the average value of CK was 397.38 IU/L, whereas for LDH it was 218.21 IU/L [
12]. In our study, the average values of CK and LDH were much higher, 1068 IU/L and 344 IU/L, respectively. As is well known, serum CK and LDH levels increase during skeletal muscle damage and degeneration. Therefore, a muscle biopsy in every case where IBM is suspected is essential for a definitive diagnosis even if there are no features of muscle weakness according to the MRC scale [
13,
26,
35,
36].
We found that skeletal muscle biopsies of IBM patients presented typical changes which have been well described by others, including hypertrophy of connective and adipose tissues, focal occurrence of necrotic fibers, RVs, a pattern of inflammatory cell infiltrations or pathological protein aggregates [
3,
4,
9,
12,
17,
37]. In most cases we saw myofiber atrophy with subsequent invasion of connective and adipose tissue. The results are in accordance with observations made by others [
12,
17,
27,
36,
37]. In a few cases hypertrophic myofibers in IBM patients were also reported [
12,
27]. However, muscle fiber hypertrophy seems to be more characteristic of skeletal muscle dystrophies than inflammatory myopathy [
38]. We have also demonstrated the presence of necrotic myofibers which could occur in muscles of IBM patients, as was described by Kierdaszuk et al. [
37]. The necrotic process is probably caused by the action of perforin and granzymes, enzymes secreted by T lymphocytes [
15]. The necrosis of single myofibers is very often associated with muscle fiber regeneration. Indeed, our biopsies found myofibers with centrally located myonuclei which are typical for muscle regeneration. Moreover, in our biopsies we saw moderately intense infiltration of mononuclear cells. To identify the type of inflammatory cell we used an immunohistochemical method, which had been previously suggested by others [
17,
37,
39]. Our results indicated that cytotoxic CD8 + T lymphocytes [
40] as well as macrophages in infiltrates are present. Current reports suggest that cN1A is a reliable marker for s-IBM, although information on this subject is still insufficient. To our knowledge, only two research teams have presented the diagnostic utility of cN1A antibody on paraffin sections [
41,
42]. Our results are the third and so far, they confirm the accumulation of cN1A protein around the vacuoles and myonuclei. Moreover, aggregates of Tau protein, beta-amyloid and apolipoprotein B were detected in the examined fibers. These results are in accordance with some other researchers who suggest that they are typical for most IBM cases [
9,
37,
39]. However, Sakai et al. [
17] found that in muscle biopsies taken from IBM patients beta-amyloid aggregates were not always present, especially in those patients with granuloma formation inside myofibers. We also found in the sarcoplasm of individual muscle fibers the central or paracentral RVs which are key elements in diagnosing s-IBM in muscles. Here, we presented unique images from the transmission electron microscope showing the real ultrastructure of RVs. To the best of our knowledge, the actual ultrastructure of vacuoles with granules at the margins has not been described. It has been suggested that the formation of RVs could be associated with the process of autophagy or myonuclei degeneration in myofibers. Recently, typical autophagic proteins and nuclear membrane proteins in these vacuoles were seen [
10,
13,
30,
43].
In some cases, tubulofilamentous inclusions within myofibers have been seen at the ultrastructural level [
3,
4]. In our study such structures were not found, implying that they do not always exist in this disease, which is consistent with diagnostic criteria of definite IBM according to Griggs (1995) [
29]. However, as others, we found typical, for IBM, whorled osmiophilic membrane debris, myelinoid bodies and lipid-like inclusion bodies which were predominant in the sarcoplasm. A few authors have reported the presence of some nuclear and cytoplasmic inclusions in IBM muscle biopsies which were located adjacent to the tubulofilamentous structures [
4] or between the myofilaments [
44]. However, in contrast to others, we did not detect inclusion bodies in the myonuclei [
2,
37]. According to other researchers [
6], at least three vacuolated fibers should be examined by electron microscopy in order to find inclusions. Interestingly, we noticed abnormalities in the triad ultrastructure, which are characteristic of centronuclear myopathy [
45,
46].
As was described above, the morphological features of the disease and detection of typical proteins are not homogenous in each case. We are aware of the main limitation of this work, namely the small number of patients, and the lack of some laboratory tests such as antibodies or magnetic resonance imaging which are not available in our university. Furthermore, they are not routinely used in regular practice. Diagnostic imaging can primarily support the differential diagnosis of inflammatory muscle disease, due to characteristic patterns of muscle involvement [
47]. This article summarizes the practical aspects of the s-IBM-diagnosis by using a combination of immunohistochemical and ultrastructural assessment. Current studies on early recognition of IBM are focused on searching for new molecular biomarkers which could be detected in skeletal muscle biopsies [
7,
9,
10,
24,
48‐
50].
Compliance with ethical standards