nach oben

Erschienen in:

20.04.2019 | Original Article

In vitro therapeutic effect of Hemiscorpius lepturus venom on tachyzoites of Toxoplasma gondii

verfasst von: L. Khaleghi Rostamkolaie, H. Hamidinejat, M. H. Razi Jalali, H. Jafari, H. Najafzadeh Varzi, M. R. Seifi Abadshapouri

Erschienen in: Journal of Parasitic Diseases | Ausgabe 3/2019

Einloggen, um Zugang zu erhalten

Abstract

Pyrimethamine which is a main anti-Toxoplasma gondii drug has a serious side and toxic effects on the host. Accordingly, the development of new treatment options for toxoplasmosis with less toxic effects, low teratogenicity and parasiticidal effect against the various stage of T. gondii are dramatically crucial. Currently, natural molecules from scorpion and snake venoms are widely used as an alternative treatment against human disease, these compounds considered to be safe and to have low toxicity in comparison with synthetic drugs. Therefore, the goal of our study was to investigate the anti-Toxoplasma gondii activities of Hemiscorpius lepturus venom. We measured cytotoxicity of H. lepturus whole venom on Vero cells as well as effectiveness of this compound on viability of T. gondii applying colorimetric assay, according to mitochondrial oxidation of the MTT reagent (Methylthiazol tetrazolium 98%). The results of this study indicated that the H. lepturus whole venom has an anti-Toxoplasma effects with less toxic effect on Vero cells. Also, the T. gondii tachyzoites were treated with H. lepturus venom reached better results in comparison with Pyrimethamine-treated group. This research will serve as a base for future studies on toxoplasmosis and suggest a role for scorpion venom in promoting natural drugs.

Ahmadpour E, Sarvi S, Hashemi Soteh MB, Sharif M, Rahimi MT, Valadan R, Tehrani M, Khalilian A, Montazeri M, Daryani A (2017) Evaluation of the immune response in BALB/c mice induced by a novel DNA vaccine expressing GRA 14 against Toxoplasmagondii. Parasite Immunol 39(4):12419CrossRef

Araujo FG, Remington JS (1992) Recent advances in the search for new drugs for treatment of toxoplasmosis. Int J Antimicrob Agents 1:153–164CrossRefPubMed

Aspinall TV, Joynson DH, Guy E, Hyde JE, Sims PF (2002) The molecular basis of sulfonamide resistance in Toxoplasma gondii and implications for the clinical management of toxoplasmosis. J Infect Dis 185(11):1637–1643CrossRefPubMed

Bahar AA, Ren D (2013) Antimicrobial peptides. Pharmaceuticals 6(12):1543–1575CrossRefPubMedPubMedCentral

Bastos LM, Júnior RJO, Silva DAO, Mineo JR, Vieira CU, Teixeira DNS, Homsi-Brandeburgo MI, Rodrigues VM, Hamaguchi A (2008) Toxoplasma gondii: effects of neuwiedase, a metalloproteinase from Bothrops neuwiedi snake venom, on the invasion and replication of human fibroblasts in vitro. Exp Parasitol 120(4):391–396CrossRefPubMed

Borges A, Silva S, den Camp HJO, Velasco E, Alvarez M, Alfonzo MJ, Jorquera A, De Sousa L, Delgado O (2006) In vitro leishmanicidal activity of Tityus discrepans scorpion venom. Parasitol Res 99(2):167–173CrossRefPubMed

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRef

Castanheira LE, de Souza DLN, Silva RJ, Barbosa B, Mineo JR, Tudini KA, Rodrigues R, Ferro EV, Rodrigues VM (2015) Insights into anti-parasitism induced by a C-type lectin from Bothrops pauloensis venom on Toxoplasma gondii. Int J Biol Macromol 74:568–574CrossRefPubMed

Conde R, Zamudio FZ, Rodtiguez MH, Possani LD (2000) Scorpine, an antimalarial and anti-bacterial agent purified from scorpion venom. Fed Eur Biochem 471:165–168CrossRef

Dehghani R, Kamiabi F, Kassiri H, Hashemi A, Mohammadzadeh N, Gharagazloo F (2018) Research article a study on litter size in several important medical scorpions species (Arachnida: Scorpionida). IR Iran J Entomol 15(3):155–160CrossRef

Del Rio-Portilla F, Flores-Solis D, Toledano Y, Rodríguez-Lima O, Cano-Sánchez P, Ernestina Ramírez-Cordero B, Landa A, de la Vega Rodríguez, Ricardo C (2016) Solution structure and anti-parasitic activity of scorpine-like peptides from Hoffmannihadrurus gertschi. FEBS Lett 590(14):2286–2296CrossRefPubMed

Di Cristina M, Marocco D, Galizi R, Proietti C, Spaccapelo R, Crisanti A (2008) Temporal and spatial distribution of Toxoplasma gondii differentiation into bradyzoites and tissue cyst formation in vivo. Infect Immun 76(8):3491–3501CrossRefPubMedPubMedCentral

Du W, Gao B, Xu J, Carmen Rodriguez M, Lanz-Mendoza H, Hernández-Rivas R, Zhu S (2010) Characterization of two linear cationic antimalarial peptides in the scorpion Mesobuthus eupeus. Biochimie 92:350–359CrossRefPubMed

Dubey JP (2008) The history of Toxoplasma gondii—the first 100 years. J Eukaryot Microbiol 55:467–475CrossRefPubMed

Dubey JP (2009) The evolution of the knowledge of cat and dog coccidia. Parasitol 136:1469–1475CrossRef

Dubey JP (2010) Toxoplasmosis of animals and humans, 2nd edn. CRC Press, Boca Raton

Dubey JP, Beattie CP (2010) Toxoplasmosis of animals and man, 2nd edn. CRC Press, Boca Raton

El-Asmar MF, Swelam N, Abdel Aal TM, Ghoneim Kh, Hodhod SS (1980) Factor(s) in the venom of scorpions toxic to Schistosoma mansoni (intestinal belharzia) cercariae. Toxicon 18(5–6):711–715CrossRefPubMed

Hassan F (1984) Production of scorpion antivenom. In: Tu AT (Ed), Handbook of toxins. Insects poisons, allergens and other invertebrate venoms, vol. 2. Marcel Dekker, New York 577–605

Heidarpour M, Ennaifer E, Ahari H, Srairi-Abid N, Borchani L, Khalili G, Amini H, Anvar AA, Boubaker S, El-Ayeb M, Shahbazzadeh D (2012) Histopathological changes induced by hemiscorpius lepturus scorpion venom in mice. Toxicon 59(3):373–378CrossRefPubMed

Jafari H, Nemati M, Haddad Molayan P, Khaleghi Rostamkolaie L, Hamidinejat H (2018) Scolicidal activity of Mesobuthus epeus venom against the protoscolices of Echinococcus granulosus. https://doi.org/10.22092/ARI.2018.121416.1213

Kalani H, Daryani A, Sharif M, Ahmadpour E, Alizadeh A, Nasrolahei M, Sarvi S, Kalani F, Faridnia R (2016) Comparison of eight cell-free media for maintenance of Toxoplasma gondii tachyzoites. Iran J Parasitol 11(1):104PubMedPubMedCentral

Kazemi-Lomedasht F, Khalaj V, Bagheri KP, Behdani M, Shahbazzadeh D (2017) The first report on transcriptome analysis of the venom gland of Iranian scorpion, Hemiscorpius lepturus. Toxicon 125:123–130CrossRefPubMed

Kim S, Fouts AE, Boothroyd JC (2007) Toxoplasma gondii dysregulates IFN-γ- inducible gene expression in human fibroblasts: insights from a genome—wide transcriptional profiling. J Immuonol 178(8):5154–5165CrossRef

Lass A, Pietkiewicz H, Modzelewska E, Dumètre A, Szostakowska B, Myjak P (2009) Detection of Toxoplasma gondii oocysts in environmental soil samples using molecular methods. Eur J Clin Microbiol Infect Dis 28(6):599–605CrossRefPubMed

Martins-Duarte ES, Urbina JA, de Souza W, Vommaro RC (2006) Antiproliferative activities of two novel quinuclidine inhibitors against Toxoplasma gondii tachyzoites in vitro. J Antimicrob Chemother 58(1):59–65CrossRefPubMed

Miller CM, Boulter NR, Smith NC (2009) The immunobiology of the innate response to Toxoplasma gondii. Int J Parasitol 39(1):23–39CrossRefPubMed

Mirzaalizadeh B, Sharif M, Daryani A, Ebrahimzadeh MA, Zargari M, Sarvi S, Mehrzadi S, Rahimi MT, Mirabediny Z, Golpour M, Montazeri M (2018) Effects of aloe vera and eucalyptus methanolic extracts on experimental toxoplasmosis in vitro and in vivo. Exp Parasitol 192:6–11CrossRefPubMed

Montazeri M, Sharif M, Sarvi S, Mehrzadi S, Ahmadpour E, Daryani A (2017) A systematic review of in vitro and in vivo activities of anti-Toxoplasma drugs and compounds (2006 to 2016). Front Microbiol 8:25PubMedPubMedCentral

Perumal SR, Stiles BG, Franco OL, Sethi G, Lim LHK (2017) Animal venoms as antimicrobial agents. Biochem Pharmacol 134:127–138CrossRef

Schmidt DR, Hogh B, Andersen O, Hansen SH, Dalhoff K, Petersen E (2006) Treatment of infants with congenital toxoplasmosis: tolerability and plasma concentrations of sulfadiazine and pyrimethamine. Eur J Pediatr 165:19–25CrossRefPubMed

Tempone AG, Melhem MSC, Prado FO, Motoie G, Hiramoto RM, Antoniazzi MM, Haddad CFB, Jared C (2007) Amphibian secretions for drug discovery studies: a search for new antiparasitic and antifungal compounds. Lett Drug Des Discov 4:67–73CrossRef

Tenter AM, Heckeroth AR, Weiss LM (2000) Toxoplasma gondii: from animals to humans. Int J Parasitol 30(12):1217–1258CrossRefPubMedPubMedCentral

Wiengcharoen J, O Hanley R, Armstrong T, Best W, Sukthana Y, Thompson RA (2007) Novel drug compounds against Neospora caninum and Toxoplasma gondii in vitro. Southeast Asian J Trop Med Public Health 38:15

Xu ZM, Li ZS, Wen MX, Peng RY, Sun L, Wu XY, Zhou LX, Tao YP, Yang L (2008) In vitro effect of medicinal scorpion on the larvae of Ancylostoma caninum. Chinese J Parasitol & Parasitic Dis 26(5):387–391

Zaware N, Sharma H, Yang J, Devambatla RKV, Queener SF, Anderson KS, Gangjee A (2013) Discovery of potent and selective inhibitors of Toxoplasma gondii thymidylate synthase for opportunistic infections. ACS Med Chem Lett 4(12):1148–1151CrossRefPubMedPubMedCentral

Zhang NZ, Wang M, Xu Y, Petersen E, Zhu XQ (2015) Recent advances in developing vaccines against Toxoplasma gondii: an update. Expert Rev Vaccines 14:1609–1621CrossRefPubMed

Titel: In vitro therapeutic effect of Hemiscorpius lepturus venom on tachyzoites of Toxoplasma gondii
verfasst von: L. Khaleghi Rostamkolaie
H. Hamidinejat
M. H. Razi Jalali
H. Jafari
H. Najafzadeh Varzi
M. R. Seifi Abadshapouri
Publikationsdatum: 20.04.2019
Verlag: Springer India
Erschienen in: Journal of Parasitic Diseases / Ausgabe 3/2019
Print ISSN: 0971-7196
Elektronische ISSN: 0975-0703
DOI: https://doi.org/10.1007/s12639-019-01113-7

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

25.04.2024 | Hypotonie | Nachrichten

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

In vitro therapeutic effect of Hemiscorpius lepturus venom on tachyzoites of Toxoplasma gondii

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Update Innere Medizin

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2019

New prevalence surveillance of Toxoplasma gondii among rodents and stray cats by ELISA avidity and nested PCR methods, Northeast of Iran

Prevalence and associated risk factors of Cystoisospora belli and Cyclospora cayetanensis infection among Iranian patients with colorectal cancer

An unusual case of Cheyletiellosis in a Persian cat and its therapeutic management

Effect of nanoparticles on the therapeutic efficacy of praziquantel against Schistosoma mansoni infection in murine models

Three previous recorded species of Dactylogyrus Diesing, 1850 (Monogenea: Dactylogyridae) infecting cultured Carassius auratus in southern Brazil

The Relation between Toxocariasis and Toxoplasmosis co-infection and the presence of Rheumatoid Factor (RF) in people with hydatidosis in Southwestern Iran, from 2013 to 2018

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

Therapiestart mit Blutdrucksenkern erhöht Frakturrisiko

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Update Innere Medizin