nach oben

Irish Journal of Medical Science (1971 -)

Erschienen in:

01.06.2015 | Original Article

Increases in interleukin-6 and interferon-gamma levels is progressive in immature rats with varicocele

verfasst von: B. Habibi, B. Seifi, S. M.-H. N. Mougahi, M. Ojaghi, H. R. Sadeghipour

Erschienen in: Irish Journal of Medical Science (1971 -) | Ausgabe 2/2015

Einloggen, um Zugang zu erhalten

Abstract

Background

Pre-pubertal varicocele can result in hypotrophy of testes, progressive deterioration of Sertoli cells and spermatogonia cell number, decrease in seminiferous diameter and cause to sperm damage.

Aims

Because of detrimental time-dependent effects of varicocele, this study describes the effects of varicocele on the levels of interleukin-6 (IL-6) and interferon-gamma in serum and testis tissue, seminiferous tubules diameter, number of Sertoli and spermatogonia cells, testis and epididymis weight and volume and sperm indices in immature rats.

Methods

Thirty-six immature rats (5–6 weeks) were assigned into six groups: three sham groups and three varicocele groups. Serum, testis, and sperm samples were collected at 9, 11, and 13 weeks after induction of varicocele or sham operation to evaluate histological parameters and levels of cytokines.

Results

Varicocele significantly caused an increase in serum and testis IL-6 and interferon-gamma, compared to related sham groups and previous varicocele groups (P < 0.05). Varicocele significantly decreased Sertoli cells and spermatogonia cell number with increasing varicocele time (P < 0.05). In the evaluation of seminiferous tubules diameter, the external, internal, and epithelium diameter were decreased compared to sham-related groups and previous varicocele groups. In the all varicocele groups, all types of sperm motility decreased compared to the related sham-operated group (P < 0.05).

Conclusions

This study suggests varicocele has a detrimental, time-dependent effect on cytokines levels and decreases Sertoli cells, spermatogonia cell number, seminiferous tubules diameter, and sperm indices.

Schlesinger MH, Wilets IF, Nagler HM (1994) Treatment outcome after varicocelectomy. A critical analysis. Urol Clin North Am 21:517–529PubMed

Kamal KM, Jarvi K, Zini A (2001) Microsurgical varicocelectomy in the era of assisted reproductive technology: influence of initial semen quality on pregnancy rates. Fertil Steril 75:1013–1016CrossRefPubMed

Marmar JL (2001) The pathophysiology of varicoceles in the light of current molecular and genetic information. Hum Reprod Update 7:461–472CrossRefPubMed

Su LM, Goldstein M, Schlegel PN (1995) The effect of varicocelectomy on serum testosterone levels in infertile men with varicoceles. J Urol 154:1752–1755CrossRefPubMed

Moretti E, Cosci I, Spreafico A et al (2009) Semen characteristics and inflammatory mediators in infertile men with different clinical diagnoses. Int J Androl 32:637–646CrossRefPubMed

Kikumura A, Ishikawa T, Norose K (2012) Kinetic analysis of cytokines, chemokines, chemokine receptors and adhesion molecules in murine ocular toxoplasmosis. Br J Ophthalmol 96:1259–1267CrossRefPubMed

Sahin Z, Celik-Ozenci C, Akkoyunlu G et al (2006) Increased expression of interleukin-1alpha and interleukin-1beta is associated with experimental varicocele. Fertil Steril 1:1265–1275CrossRef

Bian SL, Jin HB, Wang SZ et al (2007) Effects of interferon-gamma and tumor necrosis factor-alpha on the fertilizing capacity of human sperm and their mechanisms. Zhonghua Nan Ke Xue 13:681–684PubMed

Chaudhary J, Sadler-Riggleman I, Ague JM et al (2005) The helix-loop-helix inhibitor of differentiation (ID) proteins induce post-mitotic terminally differentiated Sertoli cells to re-enter the cell cycle and proliferate. Biol Reprod 72:1205–1217CrossRefPubMed

10.

Sharpe RM, McKinnell C, Kivlin C et al (2003) Proliferation and functional maturation of Sertoli cells, and their relevance to disorders of testis function in adulthood. Reproduction 125:769–784CrossRefPubMed

11.

Skoog SJ, Roberts KP, Goldstein M et al (1997) The adolescent varicocele: what’s new with an old problem in young patients? Pediatrics 100:112–121CrossRefPubMed

12.

Yamamoto M, Hibi H, Katsuno S et al (1995) Effects of varicocelectomy on testis volume and semen parameters in adolescents: a randomized prospective study. Nagoya J Med Sci 58:127–132PubMed

13.

Herbenick MA, Sprott D, Stills H et al (2008) Effects of a cyclooxygenase 2 inhibitor on fracture healing in a rat model. Am J Orthop (Belle Mead NJ) 37:E133–E137

14.

Roberts N, Puddephat MJ, McNulty V (2000) The benefit of stereology for quantitative radiology. Br J Radiol 73:679–697CrossRefPubMed

15.

Muhlfeld C, Nyengaard JR, Mayhew TM (2010) A review of state-of-the-art stereology for better quantitative 3D morphology in cardiac research. Cardiovasc Pathol Off J Soc Cardiovasc Pathol 19:65–82CrossRef

16.

Charleston JS, Hansen KR, Thyer AC et al (2007) Estimating human ovarian non-growing follicle number: the application of modern stereology techniques to an old problem. Hum Reprod 22:2103–2110CrossRefPubMed

17.

Lipshultz LI (1997) Infertility. J Urol 157:847–848CrossRefPubMed

18.

Baggiolini M, Dewald B, Moser B (1997) Human chemokines: an update. Annu Rev Immunol 15:675–705CrossRefPubMed

19.

Dousset B, Hussenet F, Daudin M et al (1997) Seminal cytokine concentrations (IL-1beta, IL-2, IL-6, sR IL-2, sR IL-6), semen parameters and blood hormonal status in male infertility. Hum Reprod 12:1476–1479CrossRefPubMed

20.

Khan SA, Khan SJ, Dorrington JH (1992) Interleukin-1 stimulates deoxyribonucleic acid synthesis in immature rat Leydig cells in vitro. Endocrinology 131:1853–1857PubMed

21.

Jegou B, Cudicini C, Gomez E et al (1995) Interleukin-1, interleukin-6 and the germ cell-Sertoli cell cross-talk. Reprod Fertil Dev 7:723–730CrossRefPubMed

22.

Hao W, Chan IH, Liu X et al (2012) Early post-operative interleukin-6 and tumor necrosis factor-alpha levels after single-port laparoscopic varicocelectomy in children. Pediatr Surg Int 28:281–286CrossRefPubMedCentralPubMed

23.

French DB, Desai NR, Agarwal A (2008) Varicocele repair: does it still have a role in infertility treatment? Curr Opin Obstet Gynecol 20:269–274CrossRefPubMed

24.

Lampiao F, du Plessis SS (2008) TNF-alpha and IL-6 affect human sperm function by elevating nitric oxide production. Reprod Biomed Online 17:628–631CrossRefPubMed

25.

Javanmard SH, Dana N (2012) The effect of interferon gamma on endothelial cell nitric oxide production and apoptosis. Adv Biomed Res 1:69PubMedCentralPubMed

26.

Perez-Rodriguez R, Roncero C, Olivan AM et al (2009) Signaling mechanisms of interferon gamma induced apoptosis in chromaffin cells: involvement of nNOS, iNOS, and NFkappaB. J Neurochem 108:1083–1096CrossRefPubMed

27.

Kim JY, Han EH, Kim HG et al (2010) Bisphenol A-induced aromatase activation is mediated by cyclooxygenase-2 up-regulation in rat testicular Leydig cells. Toxicol Lett 193:200–208CrossRefPubMed

28.

Matzkin ME, Gonzalez-Calvar SI, Mayerhofer A et al (2009) Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F(2alpha) production in hamster Leydig cells. Reproduction 138:163–175CrossRefPubMed

29.

Walker WH, Cheng J (2005) FSH and testosterone signaling in Sertoli cells. Reproduction 130:15–28CrossRefPubMed

30.

Paick JS, Park K, Kim SW et al (2012) Increased expression of hypoxiainducible factor-1alpha and connective tissue growth factor accompanied by fibrosis in the rat testis of varicocele. Actas Urol Esp 36:282–288CrossRefPubMed

31.

Trigo RV, Bergada I, Rey R et al (2004) Altered serum profile of inhibin B, ProalphaC and anti-Mullerian hormone in prepubertal and pubertal boys with varicocele. Clin Endocrinol (Oxf) 60:758–764CrossRef

32.

Agarwal A, Deepinder F, Cocuzza M et al (2007) Efficacy of varicocelectomy in improving semen parameters: new meta-analytical approach. Urology 70:532–538CrossRefPubMed

Titel: Increases in interleukin-6 and interferon-gamma levels is progressive in immature rats with varicocele
verfasst von: B. Habibi
B. Seifi
S. M.-H. N. Mougahi
M. Ojaghi
H. R. Sadeghipour
Publikationsdatum: 01.06.2015
Verlag: Springer London
Erschienen in: Irish Journal of Medical Science (1971 -) / Ausgabe 2/2015
Print ISSN: 0021-1265
Elektronische ISSN: 1863-4362
DOI: https://doi.org/10.1007/s11845-014-1167-3

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Proximale Humerusfraktur: Auch 100-Jährige operieren?

01.05.2024 DCK 2024 Kongressbericht

Mit dem demographischen Wandel versorgt auch die Chirurgie immer mehr betagte Menschen. Von Entwicklungen wie Fast-Track können auch ältere Menschen profitieren und bei proximaler Humerusfraktur können selbst manche 100-Jährige noch sicher operiert werden.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Increases in interleukin-6 and interferon-gamma levels is progressive in immature rats with varicocele

Abstract

Background

Aims

Methods

Results

Conclusions

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Proximale Humerusfraktur: Auch 100-Jährige operieren?

Die „Zehn Gebote“ des Endokarditis-Managements

Strenge Blutdruckeinstellung lohnt auch im Alter noch

Sind Frauen die fähigeren Ärzte?

Update Innere Medizin

Springer Medizin

Abstract

Background

Aims

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2015

Blood lipid profile and BMI-Z-score in adolescents with hyperuricemia

An audit of drug shortages in a community pharmacy practice

Evaluation of the efficacy of transcricoid lignocaine as adjunctive local anaesthesia for fiberoptic bronchoscopy

Incidental detection of haemoglobin (Hb) variants during high performance liquid chromatography (HPLC) analysis of HbA1c: is it time for a standardised approach to reporting?

Identifying hepatocellular carcinoma-related genes and pathways by system biology analysis

Attitudes among junior doctors towards improving the transurethral catheterisation process

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Proximale Humerusfraktur: Auch 100-Jährige operieren?

Die „Zehn Gebote“ des Endokarditis-Managements

Strenge Blutdruckeinstellung lohnt auch im Alter noch

Sind Frauen die fähigeren Ärzte?

Update Innere Medizin