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NeuroMolecular Medicine

Erschienen in:

11.03.2021 | Original Paper

Inhibition of microRNA-143-3p Attenuates Cerebral Ischemia/Reperfusion Injury by Targeting FSTL1

verfasst von: Shunda Wang, Zhenguo Liu

Erschienen in: NeuroMolecular Medicine | Ausgabe 4/2021

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Abstract

MicroRNA (miRNA) miR-143-3p has been reported to participate in the progression of myocardial ischemia/reperfusion (I/R) injury, but its function in cerebral I/R injury remains unclear. Mice were subjected to 60 min of cerebral ischemia followed by different times of reperfusion to construct an I/R injury model in vivo. Human neuroblastoma SH-SY5Y cells were treated with oxygen–glucose deprivation (OGD) for 2 h followed by different times of re-oxygenation to establish I/R injury model in vitro. Neurological deficit was assessed by a five-point score. Infarct volume was detected using 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. The expression of miR-143-3p was evaluated by qRT-PCR. The expression levels of FSTL1, Bcl-2, Bax and cleaved caspase-3 proteins were detected by western blot. The relationship between miR-143-3p and FSTL1 was explored by luciferase reporter assay. Cell viability was measured by CCK-8 assay. Cell apoptosis was evaluated by TUNEL staining and flow cytometry. MiR-143-3p was significantly upregulated during cerebral I/R injury both in vivo and in vitro. Inhibition of miR-143-3p effectively reduced I/R-induced neurological deficit score and infarct volume in vivo, and enhanced cell viability, while decreased cell apoptosis and LDH release of OGD/R-treated SH-SY5Y cells in vitro. Meanwhile, inhibition of miR-143-3p obviously decreased the expression levels of Bax and cleaved caspase-3, while increased the expression levels of Bcl-2. In addition, these changes induced by miR-143-3p inhibition in vitro was effectively reversed by silencing of FSTL1. Our results demonstrated that inhibition of miR-143-3p protected against cerebral I/R injury through targeting FSTL1.

Barthels, D., & Das, H. (2020). Current advances in ischemic stroke research and therapies. Biochim Biophys Acta Mol Basis Dis, 1866(4), 165260. https://doi.org/10.1016/j.bbadis.2018.09.012CrossRefPubMed

Bredesen, D. E., Rao, R. V., & Mehlen, P. (2006). Cell death in the nervous system. Nature, 443(7113), 796–802. https://doi.org/10.1038/nature05293CrossRefPubMedPubMedCentral

Chang, L., Shi, R., Wang, X., & Bao, Y (2020) Gypenoside A protects ischemia/reperfusion injuries by suppressing miR-143–3p level via the activation of AMPK/Foxo1 pathway. 46(3): 432–440, doi: https://doi.org/10.1002/biof.1601.

Cheng, J., Uchida, M., Zhang, W., Grafe, M. R., Herson, P. S., & Hurn, P. D. (2011). Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia. Journal of Cerebral Blood Flow and Metabolism, 31(1), 339–350. https://doi.org/10.1038/jcbfm.2010.98CrossRefPubMed

Ghafouri-Fard, S., Shoorei, H., & Taheri, M. (2020). Non-coding RNAs participate in the ischemia-reperfusion injury. Biomedicine and Pharmacotherapy, 129, 110419. https://doi.org/10.1016/j.biopha.2020.110419CrossRefPubMed

Hassan, M., Watari, H., AbuAlmaaty, A., Ohba, Y., & Sakuragi, N. (2014). Apoptosis and molecular targeting therapy in cancer. BioMed Research International, 2014, 150845. https://doi.org/10.1155/2014/150845CrossRefPubMedPubMedCentral

Hu, Z., Zhao, P., Zhang, K., Zang, L., Liao, H., & Ma, W. (2020). Hsa_circ_0011290 regulates proliferation, apoptosis and glycolytic phenotype in papillary thyroid cancer via miR-1252/ FSTL1 signal pathway. Archives of Biochemistry and Biophysics, 685, 108353. https://doi.org/10.1016/j.abb.2020.108353CrossRefPubMed

Huang, L., Ma, Q., Li, Y., Li, B., & Zhang, L. (2018). Inhibition of microRNA-210 suppresses pro-inflammatory response and reduces acute brain injury of ischemic stroke in mice. Experimental Neurology, 300, 41–50. https://doi.org/10.1016/j.expneurol.2017.10.024CrossRefPubMed

Kalogeris, T., Baines, C. P., Krenz, M., & Korthuis, R. J. (2016). Ischemia/Reperfusion. Compr Physiol, 7(1), 113–170. https://doi.org/10.1002/cphy.c160006CrossRefPubMed

Li, D., Zhang, M., Zhang, Q., Wang, Y., Song, X., & Zhang, Q. (2015). Functional recovery after acute intravenous administration of human umbilical cord mesenchymal stem cells in rats with cerebral ischemia-reperfusion injury. Intractable Rare Dis Res, 4(2), 98–104. https://doi.org/10.5582/irdr.2015.01010CrossRefPubMedPubMedCentral

Li, K. S., Jiang, W. P., Li, Q. C., Zhang, H. W., Bai, Y., Zhang, X., et al. (2020). MiR-29a in mesenchymal stem cells inhibits FSTL1 secretion and promotes cardiac myocyte apoptosis in hypoxia-reoxygenation injury. Cardiovasc Pathol, 46, 107180. https://doi.org/10.1016/j.carpath.2019.107180CrossRefPubMed

Li, W., Dong, M., Chu, L., Feng, L., & Sun, X. (2019). MicroRNA-451 relieves inflammation in cerebral ischemia-reperfusion via the Toll-like receptor 4/MyD88/NF-κB signaling pathway. Mol Med Rep, 20(4), 3043–3054. https://doi.org/10.3892/mmr.2019.10587CrossRefPubMedPubMedCentral

Liang, X., Hu, Q., Li, B., McBride, D., Bian, H., Spagnoli, P., et al. (2014). Follistatin-like 1 attenuates apoptosis via disco-interacting protein 2 homolog A/Akt pathway after middle cerebral artery occlusion in rats. Stroke, 45(10), 3048–3054. https://doi.org/10.1161/strokeaha.114.006092CrossRefPubMedPubMedCentral

Lok, J., & Martin, L. J. (2002). Rapid subcellular redistribution of Bax precedes caspase-3 and endonuclease activation during excitotoxic neuronal apoptosis in rat brain. Journal of Neurotrauma, 19(7), 815–828. https://doi.org/10.1089/08977150260190410CrossRefPubMed

Longa, E. Z., Weinstein, P. R., Carlson, S., & Cummins, R. (1989). Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke, 20(1), 84–91. https://doi.org/10.1161/01.str.20.1.84CrossRefPubMed

Lu, T. X., & Rothenberg, M. E. (2018). MicroRNA. The Journal of Allergy and Clinical Immunology, 141(4), 1202–1207. https://doi.org/10.1016/j.jaci.2017.08.034CrossRefPubMed

Mohr, A. M., & Mott, J. L. (2015). Overview of microRNA biology. Seminars in Liver Disease, 35(1), 3–11. https://doi.org/10.1055/s-0034-1397344CrossRefPubMedPubMedCentral

Murakami, K., Tanaka, M., Usui, T., Kawabata, D., Shiomi, A., Iguchi-Hashimoto, M., et al. (2012). Follistatin-related protein/follistatin-like 1 evokes an innate immune response via CD14 and toll-like receptor 4. FEBS Letters, 586(4), 319–324. https://doi.org/10.1016/j.febslet.2012.01.010CrossRefPubMed

O’Brien, J., Hayder, H., Zayed, Y., & Peng, C. (2018). Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation. Front Endocrinol (Lausanne), 9, 402. https://doi.org/10.3389/fendo.2018.00402CrossRef

Oshima, Y., Ouchi, N., Sato, K., Izumiya, Y., Pimentel, D. R., & Walsh, K. (2008). Follistatin-like 1 is an Akt-regulated cardioprotective factor that is secreted by the heart. Circulation, 117(24), 3099–3108. https://doi.org/10.1161/circulationaha.108.767673CrossRefPubMedPubMedCentral

Powers, W. J. (2020). Acute Ischemic Stroke. The New England journal of medicine, 383(3), 252–260. https://doi.org/10.1056/NEJMcp1917030CrossRefPubMed

Qian, Y., Teng, Y., Li, Y., Lin, X., Guan, M., Li, Y., et al. (2019). MiR-143-3p suppresses the progression of nasal squamous cell carcinoma by targeting Bcl-2 and IGF1R. Biochemical and Biophysical Research Communications, 518(3), 492–499. https://doi.org/10.1016/j.bbrc.2019.08.075CrossRefPubMed

Rink, C., & Khanna, S. (2011). MicroRNA in ischemic stroke etiology and pathology. Physiological Genomics, 43(10), 521–528. https://doi.org/10.1152/physiolgenomics.00158.2010CrossRefPubMed

Shi, H., Shen, H., Xu, J., Zhao, S., Yao, S., & Jiang, N. (2018). MiR-143-3p suppresses the progression of ovarian cancer. Am J Transl Res, 10(3), 866–874.PubMedPubMedCentral

Shibanuma, M., Mashimo, J., Mita, A., Kuroki, T., & Nose, K. (1993). Cloning from a mouse osteoblastic cell line of a set of transforming-growth-factor-beta 1-regulated genes, one of which seems to encode a follistatin-related polypeptide. European journal of biochemistry, 217(1), 13–19. https://doi.org/10.1111/j.1432-1033.1993.tb18212.xCrossRefPubMed

Sun, C., Jia, N., Li, R., Zhang, Z., Zhong, Y., & Han, K. (2020). miR-143-3p inhibition promotes neuronal survival in an Alzheimer’s disease cell model by targeting neuregulin-1. Folia Neuropathologica, 58(1), 10–21. https://doi.org/10.5114/fn.2020.94002CrossRefPubMed

Sun, X., Dai, G., Yu, L., Hu, Q., Chen, J., & Guo, W. (2018). miR-143-3p inhibits the proliferation, migration and invasion in osteosarcoma by targeting FOSL2. Sci Rep, 8(1), 606. https://doi.org/10.1038/s41598-017-18739-3CrossRefPubMedPubMedCentral

Tay, Y., Rinn, J., & Pandolfi, P. P. (2014). The multilayered complexity of ceRNA crosstalk and competition. Nature, 505(7483), 344–352. https://doi.org/10.1038/nature12986CrossRefPubMedPubMedCentral

Xia, C., Yang, Y., Kong, F., Kong, Q., & Shan, C. (2018). MiR-143-3p inhibits the proliferation, cell migration and invasion of human breast cancer cells by modulating the expression of MAPK7. Biochimie, 147, 98–104. https://doi.org/10.1016/j.biochi.2018.01.003CrossRefPubMed

Xiao, Y., Zhang, Y., Chen, Y., Li, J., Zhang, Z., Sun, Y., et al. (2019). Inhibition of MicroRNA-9-5p Protects Against Cardiac Remodeling Following Myocardial Infarction in Mice. Human Gene Therapy, 30(3), 286–301. https://doi.org/10.1089/hum.2018.059CrossRefPubMed

Xie, F., Li, C., Zhang, X., Peng, W., & Wen, T. (2019). MiR-143-3p suppresses tumorigenesis in pancreatic ductal adenocarcinoma by targeting KRAS. Biomedicine & Pharmacotherapy, 119, 109424. https://doi.org/10.1016/j.biopha.2019.109424CrossRef

Yu, B., Yu, M., Zhang, H., Xie, D., Nie, W., & Shi, K. (2020). Suppression of miR-143-3p contributes to the anti-fibrosis effect of atorvastatin on myocardial tissues via the modulation of Smad2 activity. Experimental and Molecular Pathology, 112, 104346. https://doi.org/10.1016/j.yexmp.2019.104346CrossRefPubMed

Zhang, M., Wu, X., Xu, Y., He, M., Yang, J., Li, J., et al. (2017). The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia. Brain, Behavior, and Immunity, 66, 332–346. https://doi.org/10.1016/j.bbi.2017.07.156CrossRefPubMed

Zhang, Z. M., Zhang, A. R., Xu, M., Lou, J., & Qiu, W. Q. (2017). TLR-4/miRNA-32-5p/FSTL1 signaling regulates mycobacterial survival and inflammatory responses in Mycobacterium tuberculosis-infected macrophages. Experimental Cell Research, 352(2), 313–321. https://doi.org/10.1016/j.yexcr.2017.02.025CrossRefPubMed

Zhong, Y., Yu, C., & Qin, W. (2019). LncRNA SNHG14 promotes inflammatory response induced by cerebral ischemia/reperfusion injury through regulating miR-136-5p /ROCK1. Cancer Gene Therapy, 26(7–8), 234–247. https://doi.org/10.1038/s41417-018-0067-5CrossRefPubMed

Zuo, X., Lu, J., Manaenko, A., Qi, X., Tang, J., Mei, Q., et al. (2019). MicroRNA-132 attenuates cerebral injury by protecting blood-brain-barrier in MCAO mice. Experimental Neurology, 316, 12–19. https://doi.org/10.1016/j.expneurol.2019.03.017CrossRefPubMed

Titel: Inhibition of microRNA-143-3p Attenuates Cerebral Ischemia/Reperfusion Injury by Targeting FSTL1
verfasst von: Shunda Wang
Zhenguo Liu
Publikationsdatum: 11.03.2021
Verlag: Springer US
Erschienen in: NeuroMolecular Medicine / Ausgabe 4/2021
Print ISSN: 1535-1084
Elektronische ISSN: 1559-1174
DOI: https://doi.org/10.1007/s12017-021-08650-6

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