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13.10.2016

Intermittent Hypoxia Contributes to the Lung Damage by Increased Oxidative Stress, Inflammation, and Disbalance in Protease/Antiprotease System

verfasst von: I. Tuleta, F. Stöckigt, U. R. Juergens, C. Pizarro, J. W. Schrickel, G. Kristiansen, G. Nickenig, D. Skowasch

Erschienen in: Lung | Ausgabe 6/2016

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Abstract

Introduction

Intermittent hypoxia as a surrogate of obstructive sleep apnea is associated with different cardiovascular complications. However, the effects of intermittent hypoxia on the lung tissue are less known. Therefore, the aim of our present study was to investigate if intermittent hypoxia may influence oxidative stress, inflammation, and protease/antiprotease system in the lung. Additionally, potential protective properties of anti-inflammatory and anti-oxidative drugs have been evaluated.

Methods

32 mice were divided into four groups: (1) intermittent hypoxia, (2) intermittent hypoxia with infliximab, (3) intermittent hypoxia with l-glutathione, and (4) normoxia. After 4 weeks, lungs and blood were collected. Levels of reactive oxygen species in the lung were calculated by L-O12-enhanced chemiluminescence. CD68-positive lung macrophages were detected by immunofluorescence. Concentrations of elastase and desmosine in lung and of alpha-1-antitrypsin in blood were calculated by means of enzyme-linked immunosorbent assay.

Results

Compared to a control, intermittent hypoxia augmented the release of free oxygen radicals, expression of CD68+ macrophages, and concentration of elastase in the lung tissue. Despite increased blood levels of protective alpha-1-antitrypsin, concentrations of desmosine—degradation product of elastin were higher versus control. The application of anti-inflammatory infliximab und anti-oxidative l-glutathione prevented at least partly the above-observed hypoxia-associated changes.

Conclusions

Intermittent hypoxia contributes to the lung damage by increased oxidative stress, inflammation, and disbalance in protease/antiprotease system. Infliximab and l-glutathione may prevent adverse hypoxia-induced lung alternations.

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Titel: Intermittent Hypoxia Contributes to the Lung Damage by Increased Oxidative Stress, Inflammation, and Disbalance in Protease/Antiprotease System
verfasst von: I. Tuleta
F. Stöckigt
U. R. Juergens
C. Pizarro
J. W. Schrickel
G. Kristiansen
G. Nickenig
D. Skowasch
Publikationsdatum: 13.10.2016
Verlag: Springer US
Erschienen in: Lung / Ausgabe 6/2016
Print ISSN: 0341-2040
Elektronische ISSN: 1432-1750
DOI: https://doi.org/10.1007/s00408-016-9946-4

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