Introduction
Congenital heart disease (CHD) is the most common type of congenital malformations, with an overall prevalence of 6-10‰ in newborns, with ~ 2-3‰ severe cases [
1]. About a quarter of CHD patients require surgery or catheterization in neonatal period, or early infancy [
2]. Timely diagnosis and treatment can greatly improve the prognosis of patients [
3]. Conversely, delayed diagnosis often leads to serious hypoxia, shock, acidosis, pneumonia, and other complications including death. Studies have reported that delayed diagnosis of critical congenital heart disease (CCHD) led to death in 1-2 of every 100,000 live births in the United States [
4]. Prenatal obstetric ultrasound can only detect ~ 30% fetuses with CHD [
5], but early neonatal diagnosis is still a big challenge.
Although echocardiography is the golden standard in CHD diagnosis, it usually takes more than 10 minutes to perform, and it is not practical to perform echocardiography for every newborn in areas where resource is limited. As an alternative, pulse oximetry (POX) is easy to operate and requires only 2 to 3 minutes to analyze the results. Besides, POX as an adjunct to current routine practice is likely to be a cost-effective strategy in the light of currently accepted thresholds [
6]. It is highly specific in detecting CCHD with moderate sensitivity, and had been widely employed [
7]. Since Dr. Guoying Huang introduced and established CHD screening system via POX in 2018, it has been gradually adopted in clinical practice in China [
8].
As a large maternal and child healthcare facility, the studied hospital delivers more than 20,000 babies annually. Providing quick, convenient, and accurate screening tools for CHD detection among newborns are crucial for the local community in the absence of echocardiologist. Since 2018, this health center implemented a new strategy of combined auscultation and POX in CHD screening among newborns. When suspected CHD babies are identified by POX or auscultation, an echocardiography was requested for confirmation. This study was the first performance report from large-scale implementation of combined auscultation and POX in CHD screenings.
Results
During 2-year study period, there were a total of 44,147 livebirths in the studied hospital. The demographic characteristics of the screened population were summarized in Table
1. The majority of the newborns were full term births delivered between 37 and 40 gestational weeks, with a median gestational age of 39 weeks. The median birth weight for newborn was 3420 g. All babies underwent screening within 24 hours after birth and cesarean section rate was 45.2%.
Table 1
Demographic characteristics of newborns
Gestational age (weeks) |
< 37 | 2691 (6.1%) | 2668 (6.1%) | 14 (4.1%) | 6 (8.6%) | 3 (6.1%) |
37-40 | 37,229 (84.3%) | 36,841 (84.3%) | 290 (85.6%) | 57 (81.4%) | 41 (83.7%) |
> 40 | 4227 (9.6%) | 4180 (9.6%) | 35 (10.3%) | 7 (10.0%) | 5 (10.2) |
Gestational age (weeks) | 39 (38-40) | 39 (38-40) | 39 (39-40) | 39 (38-39) | 39 (39-40) |
Birth Weight (g) | 3420 (3130-3720) | 3420 (3130-3720) | 3480 (3170-3800) | 3245 (3000-3515) | 3440 (3160-3790) |
Age at screening (hours) | 20 (15.0-24.0) | 20 (15.0-24.0) | 20 (16.0-24.0) | 19.5 (15.8-24.0) | 19 (15.5-24.0) |
Delivery method |
Natural birth | 24,159 (54.7%) | 23,894 (54.7%) | 205 (60.4%) | 35 (50.0%) | 25 (51.0%) |
Cesarean section | 19,946 (45.2%) | 19,754 (45.2%) | 133 (39.2%) | 35 (50.0%) | 24 (49.0%) |
Natural birth to cesarean section | 42 (0.1%) | 41 (0.1%) | 1 (0.2%) | 0 | 0 |
There were 498 newborns identified with CHD initially – 27 by POX screening and 471 by cardiac auscultation – with an overall screening rate of 1.13% among all 44,147 livebirths. Of these cases, 458 neonates were confirmed by echocardiography, making the overall diagnostic rate of CHD via echocardiogram 92.0% (including 253 male babies and 245 female babies). The screen results were presented in Table
2. The most common types of CHD were PDA (34.3%), ASD (20.5%), VSD (8.3%), and combined complications (34.5%). Rare CHD included COA, CTA PAPVC, TAPVC, TGA, and TOF, accounting for a total of 2.2% cases. Mild CHD accounted for 74% of all cases, followed by moderate CHD (15.3%), and severe CHD (10.7%). We also conducted chart review of newly diagnosed CHD in 2022, and didn’t identify additional CHD case after cross-checking with the results of newborn screening.
Table 2
Result of CHD cases
ASD | 70 (15.3) | 24 (5.2) | 0 (0) | 94 (20.5) |
ASD&PDA | 76 (16.6) | 13 (2.8) | 10 (2.2) | 99 (21.6) |
COA | 0 | 1 (0.2) | 0 | 1 (0.2) |
CTA | 0 | 1 (0.2) | 0 | 1 (0.2) |
PAPVC | 0 | 2 (0.4) | 0 | 2 (0.4) |
PDA | 127 (27.7) | 9 (2.0) | 21 (4.6) | 157 (34.3) |
TAPVC | 0 | 0 | 3 (0.7) | 3 (0.6) |
TGA | 0 | 0 | 2 (0.4) | 2 (0.4) |
TOF | 0 | 0 | 2 (0.4) | 2 (0.4) |
VSD | 27 (5.9) | 7 (1.5) | 4 (0.9) | 38 (8.3) |
VSD&ASD | 14 (3.1) | 5 (1.1) | 2 (0.4) | 21 (4.6) |
VSD&ASD&PDA | 9 (2.0) | 3 (0.7) | 2 (0.4) | 14 (3.1) |
VSD&PDA | 16 (3.5) | 5 (1.1) | 3 (0.7) | 24 (5.2) |
Total | 339 (74.0) | 70 (15.3) | 49 (10.7) | 458 (100) |
Of the 458 confirmed CHD subjects, cardiac auscultation alone detected majority of 438 CHD cases (95.6%) and POX only screened 20 (4.4%) cases. However, no single incidence of CHD was recognized by both auscultation and POX screening (Table
3). A potential explanation for this result is that auscultation detected most of the common types of CHD neonates, but POX detected the rare, yet critical CHD types, including COA, CTA, PAPVC, TAPVC, TGA, and TOF. A similar finding was observed in a new set of data from 2020 (data not shown).
Table 3
Detection for CHD types
Study Period Data |
ASD | 94 | 94 | 0 | 0 |
ASD&PDA | 99 | 99 | 0 | 0 |
COA | 1 | 0 | 1 | 0 |
CTA | 1 | 0 | 1 | 0 |
PAPVC | 2 | 0 | 2 | 0 |
PDA | 157 | 154 | 3 | 0 |
TAPVC | 3 | 0 | 3 | 0 |
TGA | 2 | 0 | 2 | 0 |
TOF | 2 | 0 | 2 | 0 |
VSD | 38 | 35 | 3 | 0 |
VSD&ASD | 21 | 20 | 1 | 0 |
VSD&ASD&PDA | 14 | 12 | 2 | 0 |
VSD&PDA | 24 | 24 | 0 | 0 |
In Total | 458 | 438 | 20 | 0 |
First Half of 2020 Data |
ASD | 6 | 6 | 0 | 0 |
VSD | 8 | 8 | 0 | 0 |
PDA | 10 | 10 | 0 | 0 |
ASD&VSD | 2 | 2 | 0 | 0 |
VSD&PDA | 2 | 2 | 0 | 0 |
TOF | 1 | 0 | 1 | 0 |
TAPVC | 1 | 0 | 1 | 0 |
In total | 30 | 28 | 2 | 0 |
POX screening alone had a low accuracy of 74.07% in positive predict value (PPV). Auscultation alone functioned well in terms of PPV and negative predict value (NPV) (92.99 and 99.95%, respectively), but the addition of POX improved the overall screening performance to 100% NPV (Table
4).
Table 4
Screening accuracy for CHD newborns (n = 44,147)
True positives | 20 | 438 | 458 |
False negatives | 438 | 20 | 0 |
False positives | 7 | 33 | 40 |
True negatives | 43,682 | 43,656 | 43,649 |
Sensitivity | 4.37% | 95.63% | 100.00% |
Specificity | 99.98% | 99.92% | 99.91% |
Positive Predict Value | 74.07% | 92.99% | 91.97% |
Negative Predict Value | 99.01% | 99.95% | 100% |
As PDA and ASD were not included in prenatal screening by ultrasonography, they were excluded when tracing back prenatal ultrasound screening. Of the remaining 108 cases, 37 (34.3%) fetuses were diagnosed with CHD, including 35 cases of VSD, 1 case of COA, and 1 case of TOF (Table
5).
Table 5
Prenatal trace back of confirmed CHD cases excluding PDA and ASD cases (n = 108)
Prenatal identification of CHD |
VSD | 35 | 32.41 |
COA | 1 | 0.93 |
TOF | 1 | 0.93 |
Prenatal identification of non-CHD | 51 | 47.22 |
Unclear ultrasound image | 3 | 2.78 |
No prenatal screening | 14 | 12.96 |
Lost to trace back | 3 | 2.78 |
Discussion
This was the first retrospective report of large-scale clinical implementation of combined POX and cardiac auscultation in routine CHD screening for all newborn infants within 24 hours after delivery. Within a two-year period, we identified 458 CHD neonates from 44,147 live births, of which 74% neonates are diagnosed with mild CHD, 15.3% with moderate CHD, and 10.7% with major CHD. POX in conjunction with clinical auscultation resulted in a high detection rate of serious CHD.
The most intriguing finding in this clinical evaluation was that there was no overlap of CHD spectrums between POX detection and auscultation detection. In contrast to common CHDs detected by auscultation, POX-detected CHDs were rare and critical, including COA, CTA, PAPVC, TAPVC, TGA, and TOF, which were consistent with the primary and second target lesions presenting at least mild hypoxemia during the neonatal period [
11]. This observation was different from two previous research with significant overlaps between two screening techniques [
8,
12]. The dynamic changes of pulmonary arterial pressure in newborns in combination of all screenings conducted within the first 24 hours after delivery likely contributed to the difference between our findings and the previous observations. In fetal period, pulmonary circulation is not in use. Instead, it is occupied by amniotic fluid and full of pressure. After birth, because postnatal circulation requires inhalation of air, lung aeration initiates and clears the airway fluid, accompanied by decreasing pulmonary arterial pressure from 60 mmHg at birth to about 30 mmHg at 24 hours of age [
13‐
15]. Since all of our screenings were conducted within the first 24 hours of delivery, which was different from the 72-hour window used in previous studies, it might be difficult to detect abnormal heart murmur when pulmonary arterial pressure still maintained relatively high levels. Moreover, in one of two previous studies with a large population of 167,190 asymptomatic newborn infants, most POX-recognizable CHD cases were only detected via auscultation [
12]. Since this study was a multicenter research investigating the accuracy and feasibility of implementing cardiac auscultation and POX as a screening method in China, there might be large variations in the POX measurement among different hospitals studied for different researches, resulting in low detection rate by POX in other studies. Our findings manifested the complementary aspects of POX and auscultation in early screening of CHD among newborns, shedding light on the effectiveness of combined use of both methods in comprehensive neonatal screening, especially regions lacking echocardiologists.
Meanwhile, the commonality of certain types of CHD was different: PDA, VSD, and ASD accounting for 97.6% of total CHD cases in our study and ~ 91.1% in previous studies. The difference in incidence rate and the CHD spectrum could be explained by differences in prenatal management among different hospitals [
8,
12]. Tertiary referral hospitals have more medical resources and better prenatal screening and management systems. Pregnant women registered at these hospitals often received additional prenatal ultrasound screening, which can increase the chance of prenatal diagnosis of major CHD, usually followed with termination of pregnancy. Contrasting to previous multicenter researches which collected data from a wide range of hospitals, our study was conducted at a single, tertiary referral hospital which had better prenatal management systems, prenatal screenings and adequately trained medical staffs [
16].
Some mild congenital defects, including small muscular VSD, ASD and PDA, would become less noticeable or even close spontaneously without intervention [
17]. Owing to the natural closure, these neonates should not be included when calculating CHD incidence, or if counted, it will increase the incidence rate at different stages. Moreover, due to limited resource, our study only considered observable CHD cases before discharge, whereas other studies offered follow up statistics among neonates after discharge. Thus, previously misdiagnosed or undiagnosed neonates would be determined to have CHD and added to the total CHD incidence later in the study. Furthermore, some studies would exclude CHD cases that were screened out in prenatal ultrasonography and confirmed in postnatal echocardiography, and symptomatic CHD neonates such as cyanotic subjects would not be included, either. As a whole, these limitations explain the observable differences in overall CHD occurrence and the major type of diagnosed CHD between our study and the previous studies.
Since this was a retrospective report of large-scale clinical implementation of combined POX and cardiac auscultation in routine CHD screening and we didn’t conduct follow-up investigation, it was possible some CHD infants were missed in screening. We therefore conducted a chart review of newly diagnosed CHD in 2022, and didn’t identify additional CHD case after cross-checking with the results of newborn screening. We presumed that the studied hospital was the major tertiary hospital for pediatric referrals, and CHD children missed in newborn screening could be identified from chart review. Although it was possible that missed CHD children could be referred to another hospital, the number of missed CHD cases should be small.
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit
http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (
http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.