nach oben

Lasers in Medical Science

Erschienen in:

03.11.2017 | Original Article

Laser-induced autofluorescence-based objective evaluation of burn tissue repair in mice

verfasst von: Bharath Rathnakar, Bola Sadashiva Satish Rao, Vijendra Prabhu, Subhash Chandra, Krishna Kishore Mahato

Erschienen in: Lasers in Medical Science | Ausgabe 4/2018

Einloggen, um Zugang zu erhalten

Abstract

Management of burn injuries are a growing concern, especially in determining the progression of healing. Several techniques are being practiced in clinics and have been considered all-time standard approaches to determine pre- and post-treatment outcomes of a healthy healing. However, these kinds of methods involve repeated biopsies and thereby hindering tissue repair. In view of this, our perspective was to develop a non-invasive tool in an attempt to provide a solution to determine the progression of healing, in vivo. Hence, the present study was designed to investigate the ability of laser-induced fluorescence (LIF) to monitor the variations in collagen intensity at various time points (0, 2, 6, 12, 18, 24, and 30 days) during burn tissue repair in mice, post low-power laser therapy (LPLT). The spectral findings demonstrated a significant change in collagen intensity as observed on day 24 (p < 0.05) and 30 (p < 0.01), when treated with LPLT (830 nm 3 J/cm²) as compared to untreated control. From the observation, it was evident that the LIF could objectively monitor the progression of burn tissue repair in vivo.

Schreml S, Szeimies RM, Prantl L, Landthaler M, Babilas P (2010) Wound healing in the 21st century. J Am Acad Dermatol 63(5):866–881. https://doi.org/10.1016/j.jaad.2009.10.048 CrossRefPubMed

Guo S, Dipietro LA (2010) Factors affecting wound healing. J Dent Res 89(3):219–229. https://doi.org/10.1177/0022034509359125 CrossRefPubMedPubMedCentral

Kao CC, Garner WL (2000) Acute burns. Plast Reconstr Surg 101(7):2482–2493CrossRefPubMed

Velnar T, Bailey T, Smrkolj V (2009) The wound healing process: an overview of the cellular and molecular mechanisms. The Journal of international medical research 37(5):1528–1542. https://doi.org/10.1177/147323000903700531 CrossRefPubMed

Spanholtz TA, Theodorou P, Amini P, Spilker G (2009) Severe burn injuries: acute and long-term treatment. Deutsches Arzteblatt international 106(38):607–613. https://doi.org/10.3238/arztebl.2009.0607 PubMedPubMedCentral

Huang YY, Sharma SK, Carroll J, Hamblin MR (2011) Biphasic dose response in low level light therapy—an update. Dose-response : a publication of International Hormesis Society 9(4):602–618. https://doi.org/10.2203/dose-response.11-009.Hamblin CrossRef

Karu TI (2008) Mitochondrial signaling in mammalian cells activated by red and near-IR radiation. Photochem Photobiol 84(5):1091–1099. https://doi.org/10.1111/j.1751-1097.2008.00394.x CrossRefPubMed

Fan WPL, Rashid M, Enoch S (2010) Current advances in modern wound healing. Wounds UK 6(3):22–36

Hopkins JT, McLoda TA, Seegmiller JG, David Baxter G (2004) Low-level laser therapy facilitates superficial wound healing in humans: a triple-blind, sham-controlled study. J Athl Train 39(3):223–229PubMedPubMedCentral

10.

Rangaraj A, Harding K, Leaper D (2011) Role of collagen in wound management. Wounds uk 7(2):54–63

11.

Prabhu V, Rao SB, Chandra S, Kumar P, Rao L, Guddattu V, Satyamoorthy K, Mahato KK (2012) Spectroscopic and histological evaluation of wound healing progression following low level laser therapy (LLLT). J Biophotonics 5(2):168–184. https://doi.org/10.1002/jbio.201100089 CrossRefPubMed

12.

Hegde VN, Prabhu V, Rao SB, Chandra S, Kumar P, Satyamoorthy K, Mahato KK (2011) Effect of laser dose and treatment schedule on excision wound healing in diabetic mice. Photochem Photobiol 87(6):1433–1441. https://doi.org/10.1111/j.1751-1097.2011.00991.x CrossRefPubMed

13.

Vázquez-Ortíz F, Morón-Fuenmayor O, González-Méndez N (2004) Hydroxyproline measurement by HPLC: improved method of total collagen determination in meat samples. J Liq Chromatogr Relat Technol 27(17):2771–2780CrossRef

14.

Starborg T, Lu Y, Kadler KE, Holmes DF (2008) Electron microscopy of collagen fibril structure in vitro and in vivo including three-dimensional reconstruction. Methods Cell Biol 88:319–345. https://doi.org/10.1016/S0091-679X(08)00417-2 CrossRefPubMed

15.

Rathnakar B, Rao BS, Prabhu V, Chandra S, Rai S, Rao AC, Sharma M, Gupta PK, Mahato KK (2016) Photo-biomodulatory response of low-power laser irradiation on burn tissue repair in mice. Lasers Med Sci 31(9):1741–1750. https://doi.org/10.1007/s10103-016-2044-2 CrossRefPubMed

16.

Prabhu V, Rao SB, Fernandes EM, Rao AC, Prasad K, Mahato KK (2014) Objective assessment of endogenous collagen in vivo during tissue repair by laser induced fluorescence. PLoS One 9(5):e98609. https://doi.org/10.1371/journal.pone.0098609 CrossRefPubMedPubMedCentral

17.

Korol RM, Finlay HM, Josseau MJ, Lucas AR, Canham PB (2007) Fluorescence spectroscopy and birefringence of molecular changes in maturing rat tail tendon. J Biomed Opt 12(2):024011. https://doi.org/10.1117/1.2714055 CrossRefPubMed

18.

Richards-Kortum R, Sevick-Muraca E (1996) Quantitative optical spectroscopy for tissue diagnosis. Annu Rev Phys Chem 47:555–606. https://doi.org/10.1146/annurev.physchem.47.1.555 CrossRefPubMed

19.

Konig K (2008) Clinical multiphoton tomography. J Biophotonics 1(1):13–23. https://doi.org/10.1002/jbio.200710022 CrossRefPubMed

20.

Patalay R, Talbot C, Alexandrov Y, Munro I, Neil MA, Konig K, French PM, Chu A, Stamp GW, Dunsby C (2011) Quantification of cellular autofluorescence of human skin using multiphoton tomography and fluorescence lifetime imaging in two spectral detection channels. Biomedical optics express 2(12):3295–3308. https://doi.org/10.1364/BOE.2.003295 CrossRefPubMedPubMedCentral

21.

Teale FW, Weber G (1957) Ultraviolet fluorescence of the aromatic amino acids. The Biochemical journal 65(3):476–482CrossRefPubMedPubMedCentral

22.

Weber G (1960) Fluorescence-polarization spectrum and electronic-energy transfer in tyrosine, tryptophan and related compounds. The Biochemical journal 75:335–345CrossRefPubMedPubMedCentral

23.

Palero JA, de Bruijn HS, van der Ploeg van den Heuvel A, Sterenborg HJ, Gerritsen HC (2007) Spectrally resolved multiphoton imaging of in vivo and excised mouse skin tissues. Biophys J 93(3):992–1007. https://doi.org/10.1529/biophysj.106.099457 CrossRefPubMedPubMedCentral

24.

Cothren RM, Richards-Kortum R, Sivak MV Jr, Fitzmaurice M, Rava RP, Boyce GA, Doxtader M, Blackman R, Ivanc TB, Hayes GB et al (1990) Gastrointestinal tissue diagnosis by laser-induced fluorescence spectroscopy at endoscopy. Gastrointest Endosc 36(2):105–111CrossRefPubMed

25.

Hariri LP, Tumlinson AR, Besselsen DG, Utzinger U, Gerner EW, Barton JK (2006) Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model. Lasers Surg Med 38(4):305–313. https://doi.org/10.1002/lsm.20305 CrossRefPubMed

26.

Panjehpour M, Julius CE, Phan MN, Vo-Dinh T, Overholt S (2002) Laser-induced fluorescence spectroscopy for in vivo diagnosis of non-melanoma skin cancers. Lasers Surg Med 31(5):367–373. https://doi.org/10.1002/lsm.10125 CrossRefPubMed

27.

Gillies R, Zonios G, Anderson RR, Kollias N (2000) Fluorescence excitation spectroscopy provides information about human skin in vivo. The Journal of investigative dermatology 115(4):704–707. https://doi.org/10.1046/j.1523-1747.2000.00091.x CrossRefPubMed

28.

Kollias N, Gillies R, Moran M, Kochevar IE, Anderson RR (1998) Endogenous skin fluorescence includes bands that may serve as quantitative markers of aging and photoaging. The Journal of investigative dermatology 111(5):776–780. https://doi.org/10.1046/j.1523-1747.1998.00377.x CrossRefPubMed

29.

Baraga JJ, Rava RP, Taroni P, Kittrell C, Fitzmaurice M, Feld MS (1990) Laser induced fluorescence spectroscopy of normal and atherosclerotic human aorta using 306-310 nm excitation. Lasers Surg Med 10(3):245–261CrossRefPubMed

30.

Marcu L, Fishbein MC, Maarek JM, Grundfest WS (2001) Discrimination of human coronary artery atherosclerotic lipid-rich lesions by time-resolved laser-induced fluorescence spectroscopy. Arterioscler Thromb Vasc Biol 21(7):1244–1250CrossRefPubMed

31.

Koenig K, Schneckenburger H (1994) Laser-induced autofluorescence for medical diagnosis. J Fluoresc 4(1):17–40. https://doi.org/10.1007/BF01876650 CrossRefPubMed

32.

Monici M (2005) Cell and tissue autofluorescence research and diagnostic applications. Biotechnol Annu Rev 11:227–256. https://doi.org/10.1016/S1387-2656(05)11007-2 CrossRefPubMed

33.

Broughton G 2nd, Janis JE, Attinger CE (2006) The basic science of wound healing. Plast Reconstr Surg 117(7 Suppl):12S–34S. https://doi.org/10.1097/01.prs.0000225430.42531.c2 CrossRefPubMed

34.

Diegelmann RF, Evans MC (2004) Wound healing: an overview of acute, fibrotic and delayed healing. Frontiers in bioscience : a journal and virtual library 9:283–289CrossRef

35.

Prabhu V, Rao SB, Rao NB, Aithal KB, Kumar P, Mahato KK (2010) Development and evaluation of fiber optic probe-based helium-neon low-level laser therapy system for tissue regeneration—an in vivo experimental study. Photochem Photobiol 86(6):1364–1372. https://doi.org/10.1111/j.1751-1097.2010.00791.x CrossRefPubMed

36.

Abrahamse H (2015) Stimulation of cellular proliferation and migration: is it a viable measure of photobiomodulation? Photomed Laser Surg 33(7):347–348. https://doi.org/10.1089/pho.2015.3945 CrossRefPubMed

37.

Fiorio FB, Albertini R, Leal-Junior EC, de Carvalho Pde T (2014) Effect of low-level laser therapy on types I and III collagen and inflammatory cells in rats with induced third-degree burns. Lasers Med Sci 29(1):313–319. https://doi.org/10.1007/s10103-013-1341-2 CrossRefPubMed

38.

Tatmatsu-Rocha JC, Ferraresi C, Hamblin MR, Damasceno Maia F, do Nascimento NR, Driusso P, Parizotto NA (2016) Low-level laser therapy (904 nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin. J Photochem Photobiol B 164:96–102. https://doi.org/10.1016/j.jphotobiol.2016.09.017 CrossRefPubMed

39.

Trajano ET, da Trajano LA, Dos Santos Silva MA, Venter NG, de Porto LC, de Fonseca A, Monte-Alto-Costa A (2015) Low-level red laser improves healing of second-degree burn when applied during proliferative phase. Lasers Med Sci 30(4):1297–1304. https://doi.org/10.1007/s10103-015-1729-2 CrossRefPubMed

40.

Menter JM (2006) Temperature dependence of collagen fluorescence. Photochemical and photobiological sciences: Official journal of the European Photochemistry Association and the European Society for Photobiology 5(4):403–410. https://doi.org/10.1039/b516429j CrossRef

41.

Martin P, Leibovich SJ (2005) Inflammatory cells during wound repair: the good, the bad and the ugly. Trends Cell Biol 15(11):599–607. https://doi.org/10.1016/j.tcb.2005.09.002 CrossRefPubMed

42.

Heng MC (2011) Wound healing in adult skin: aiming for perfect regeneration. Int J Dermatol 50(9):1058–1066. https://doi.org/10.1111/j.1365-4632.2011.04940.x CrossRefPubMed

43.

Stadelmann WK, Digenis AG, Tobin GR (1998) Physiology and healing dynamics of chronic cutaneous wounds. Am J Surg 176(2A Suppl):26S–38SCrossRefPubMed

44.

Deka G, Wu WW, Kao FJ (2013) In vivo wound healing diagnosis with second harmonic and fluorescence lifetime imaging. J Biomed Opt 18(6):061222CrossRef

45.

Alfano RR (2011) Advances in optical biopsy for cancer diagnosis. Technology in cancer research & treatment 10(2):101. https://doi.org/10.7785/tcrt.2012.500184 CrossRef

46.

Greaves NS, Benatar B, Whiteside S, Alonso-Rasgado T, Baguneid M, Bayat A (2014) Optical coherence tomography: a reliable alternative to invasive histological assessment of acute wound healing in human skin? Br J Dermatol 170(4):840–850. https://doi.org/10.1111/bjd.12786 CrossRefPubMed

47.

Jain R, Calderon D, Kierski PR, Schurr MJ, Czuprynski CJ, Murphy CJ, McAnulty JF, Abbott NL (2014) Raman spectroscopy enables noninvasive biochemical characterization and identification of the stage of healing of a wound. Anal Chem 86(8):3764–3772. https://doi.org/10.1021/ac500513t CrossRefPubMedPubMedCentral

48.

Cicchi R, Kapsokalyvas D, De Giorgi V, Maio V, Van Wiechen A, Massi D, Lotti T, Pavone FS (2010) Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy. J Biophotonics 3(1–2):34–43. https://doi.org/10.1002/jbio.200910062 PubMed

49.

Lakowicz JR, Szmacinski H, Nowaczyk K, Berndt KW, Johnson M (1992) Fluorescence lifetime imaging. Anal Biochem 202(2):316–330CrossRefPubMed

50.

Moerner WE (2007) New directions in single-molecule imaging and analysis. Proc Natl Acad Sci U S A 104(31):12596–12602. https://doi.org/10.1073/pnas.0610081104 CrossRefPubMedPubMedCentral

Titel: Laser-induced autofluorescence-based objective evaluation of burn tissue repair in mice
verfasst von: Bharath Rathnakar
Bola Sadashiva Satish Rao
Vijendra Prabhu
Subhash Chandra
Krishna Kishore Mahato
Publikationsdatum: 03.11.2017
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 4/2018
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-017-2371-y

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2018

Effects of corticopuncture (CP) and low-level laser therapy (LLLT) on the rate of tooth movement and root resorption in rats using micro-CT evaluation

When is the best moment to apply photobiomodulation therapy (PBMT) when associated to a treadmill endurance-training program? A randomized, triple-blinded, placebo-controlled clinical trial

Photobiomodulation improves motor response in patients with spinal cord injury submitted to electromyographic evaluation: randomized clinical trial

Case series about ex vivo identification of squamous cell carcinomas by laser-induced autofluorescence and Fourier transform infrared spectroscopy

Transurethral vaporesection of prostate: diode laser or thulium laser?

Curcumin-mediated anti-microbial photodynamic therapy against Candida dubliniensis biofilms