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01.03.2010 | Original Paper

Lymphocyte subsets in peripheral blood of patients with psoriasis before and after treatment with leishmania antigens

verfasst von: Jose Antonio O’Daly, Beatriz Rodriguez, Tania Ovalles, Civel Pelaez

Erschienen in: Archives of Dermatological Research | Ausgabe 2/2010

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Abstract

Peripheral blood mononuclear cells (PBMC) collected from subjects prior to treatment and post-treatment with a vaccine composed of leishmania antigens were analyzed by flow cytometry. Upon analysis, it was noticed that lymphocyte subsets (LS) varied with psoriasis area and severity index (PASI) range (1–10, 11–20 and 21–72). Pre-treatment absolute values of gated LS were as follows. CD4+CD8−, CD3+CD8−, CD8+CD3+, CD8+CD4− and CD8+HLA− decreased in PBMC as PASI increased, suggesting migration from the blood to the skin. Contrary to the previous finding, the following LS, CD8+HLA+ and HLA+CD8−, and membrane surface immunoglobulin IgA+, IgD+ and IgM+ increased in PBMC as PASI increased, suggesting activation and proliferation by unknown antigens. After treatment with seven doses of AS100, the following LS, CD3+CD8−, CD8+CD3−, HLA+CD8−, CD8+HLA+ and CD4+CD8−, increased, while CD8+CD3+, CD8+HLA−, CD19 and CD8+CD4+ decreased in PBMC.

Abul H, Mahmoud F, Al Saleh Q et al (2002) Profiles of activated T lymphocytes in peripheral blood of Kuwaiti psoriasis vulgaris patients. J Dermatol 29:202–208PubMed

Bachelez H (2005) Immunopathogenesis of psoriasis: recent insights on the role of adaptive and innate immunity. J Autoimmun 25:69–73CrossRefPubMed

Baker BS, Swain AF, Valdimarsson H et al (1984) T-cell subpopulations in the blood and skin of patients with psoriasis. Br J Dermatol 110:37–44CrossRefPubMed

Blue ML, Daley JF, Levine H et al (1985) Coexpression of T4 and T8 on peripheral blood T cells demonstrated by two-color fluorescence flow cytometry. J Immunol 134:2281–2286PubMed

Bock O, Kreiselmeyer I, Mrowietz U (2001) Expression of dipeptidyl-peptidase IV (CD26) on CD8+ T cells is significantly decreased in patients with psoriasis vulgaris and atopic dermatitis. Exp Dermatol 10:414–419CrossRefPubMed

Christophers E, Mrowietz U (2003) Psoriasis. In: Freedberg IM, Eisen AZ, Wolff KK, Austen F, Goldsmith LA, Si Katz (eds) Fitzpatrick’s dermatology in general medicine, 6th edn. McGraw-Hill, New York, pp 407–427

Endharti AT, Rifa’ IM, Shi Z et al (2005) Cutting Edge: CD8⁺CD122⁺ regulatory T cells produce IL-10 to suppress IFN-γ production and proliferation of CD8⁺ T cells. J Immunol 175:7093–7097PubMed

Gudjonsson JE, Johnston A, Sigmundsdottir H et al (2004) Immunopathogenic mechanisms in psoriasis. Clin Exp Immunol 135:1–8CrossRefPubMed

Huang X, Yang Y (2006) The fate of effector CD8 T cells in vivo is controlled by the duration of antigen stimulation. Immunology 118:361–371CrossRefPubMed

10.

Kemp M, Hansen MB, Theander TG (1992) Recognition of leishmaniaantigens by T lymphocytes from nonexposed individuals. Infect Immun 60:2246–2251PubMed

11.

Kitchen SG, Whitmire JK, Jones NR et al (2005) The CD4 molecule on CD8⁺ T lymphocytes directly enhances the immune response to viral and cellular antigens. Proc Natl Acad Sci USA 102:3794–3799CrossRefPubMed

12.

Koch HJ, Wollina U, Seyfarth M et al (1990) Detection of IgA in diseased skin and serum of patients with psoriasis vulgaris. Dermatol Monatsschr 176:225–231PubMed

13.

Krueger JG, Bowcock A (2005) Psoriasis pathophysiology: current concepts of pathogenesis. Ann Rheum Dis 64(Suppl 2):ii30–ii36CrossRefPubMed

14.

Langewouters AMG, van Erp PEJ, de Jong EMGJ et al (2008) Lymphocyte subsets in peripheral blood of patients with moderate-to-severe versus mild plaque psoriasis. Arch Dermatol Res 300:107–113CrossRefPubMed

15.

Laux I, Khoshnan A, Tindell C et al (2000) Response differences between human CD4+ and CD8+ T-cells during CD28 costimulation: implications for immune cell-based therapies and studies related to the expansion of double-positive T-cells during aging. Clin Immunol 96:187–197CrossRefPubMed

16.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193(1):265–275PubMed

17.

Nair RP, Ding J, Duffin KC et al (2009) Psoriasis bench to bedside. Arch Dermatol 145:462–464CrossRefPubMed

18.

Nickoloff BJ, Nestle FO (2004) Recent insights into the immunopathogenesis of psoriasis provide new therapeutic opportunities. J Clin Invest 113:1664–1675PubMed

19.

O’Daly JA, Lezama R, Gleason J (2009) Isolation of Leishmania amastigote protein fractions which induced lymphocyte stimulation and remission of psoriasis. Arch Dermatol Res 301:411–427CrossRefPubMed

20.

O’Daly JA, Lezama R, Rodriguez PJ et al (2009) Antigens from Leishmania amastigotes induced clinical remission of psoriasis. Arch Dermatol Res 301:1–13CrossRefPubMed

21.

O’Daly JA, Rodriguez MB (1988) Differential growth requirements of several leishmania spp in chemically defined culture media. Acta Trop Basel 45:109–126PubMed

22.

Ozturk G, Erbas D, Gelir E et al (2001) Natural killer cell activity, serum immunoglobulins, complement proteins, and zinc levels in patients with psoriasis vulgaris. Immunol Invest 30:181–190CrossRefPubMed

23.

Prinz JC, Vollmer S, Boehncke WH et al (1999) Selection of conserved TCR VDJ rearrangements in chronic psoriatic plaques indicates a common antigen in psoriasis vulgaris. Eur J Immunol 29:3360–3368CrossRefPubMed

24.

Rantakokko K, Rimpilainen M, Uksila J et al (1997) Antibodies to streptococcal cell wall in psoriatic arthritis and cutaneous psoriasis. Clin Exp Rheumatol 15:399–404PubMed

25.

Reibke R, Garbi N, Ganss R et al (2006) CD8⁺ regulatory T cells generated by neonatal recognition of peripheral self-antigen. Proc Natl Acad Sci USA 103:15142–15147CrossRefPubMed

26.

Sabat R, Philipp S, Hoflich C et al (2007) Immunopathogenesis of psoriasis. Exp Dermatol 16:779–798CrossRefPubMed

27.

Sala P, Tonutti E, Feruglio C et al (1993) Persistent expansions of CD4⁺ CD8⁺ peripheral blood T cells. Blood 82:1546–1552PubMed

28.

Sigmundsdottir H, Gudjonsson JE, Jonsdottir I et al (2001) The frequency of CLA+ CD8+ T cells in the blood of psoriasis patients correlates closely with the severity of their disease. Clin Exp Immunol 126:365–369CrossRefPubMed

29.

Simelyte E, Rimpilainen M, Zhang X et al (2003) Role of peptidoglycan subtypes in the pathogenesis of bacterial cell wall arthritis. Ann Rheum Dis 62:976–982CrossRefPubMed

30.

Smith PK, Krohn RI, Hermanson GT et al (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150:76–85CrossRefPubMed

31.

Trautmann A, Rückert B, Schmid-Grendelmeier P (2003) Human CD8 T cells of the peripheral blood contain a low CD8 expressing cytotoxic/effector subpopulation. Immunology 108:305–312CrossRefPubMed

32.

Van Lingen RG, van de Kerkhof PCM, de Jong EMGJ et al (2008) Reduced CD26bright expression of peripheral blood CD8+ T-cell subsets in psoriatic patients. Exp Dermatol 17:343–348CrossRefPubMed

33.

Wu Y, Zheng Z, Jiang Y et al (2009) The specificity of T cell regulation that enables self-nonself discrimination in the periphery. Proc Natl Acad Sci USA 106:534–539CrossRefPubMed

Titel: Lymphocyte subsets in peripheral blood of patients with psoriasis before and after treatment with leishmania antigens
verfasst von: Jose Antonio O’Daly
Beatriz Rodriguez
Tania Ovalles
Civel Pelaez
Publikationsdatum: 01.03.2010
Verlag: Springer-Verlag
Erschienen in: Archives of Dermatological Research / Ausgabe 2/2010
Print ISSN: 0340-3696
Elektronische ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-009-0992-0

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Lymphocyte subsets in peripheral blood of patients with psoriasis before and after treatment with leishmania antigens

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