Erschienen in:
01.10.2015 | Brief Report
Characterization of the nuclear import and export signals of pseudorabies virus UL31
verfasst von:
Meili Li, Si Jiang, Jinlin Wang, Chuncong Mo, Zhancheng Zeng, Yanjia Yang, Chunke Chen, Xiaowei Li, Wei Cui, Jinlu Huang, Tao Peng, Mingsheng Cai
Erschienen in:
Archives of Virology
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Ausgabe 10/2015
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Abstract
The pseudorabies virus (PRV) UL31 protein (pUL31) is a homologue of the herpes simplex virus 1 pUL31, which is a multifunctional protein that is important for HSV-1 infection. However, little is known concerning the subcellular localization signal of PRV UL31. Here, by transfection with a series of PRV UL31 deletion mutants fused to an enhanced yellow fluorescent protein (EYFP) gene, a bipartite nuclear localization signal (NLS) and a PY motif NLS of UL31 were identified and mapped to amino acids (aa) 4 to 20 (RRRLLRRKSSAARRKTL) and aa 21 to 34 (TRAARDRYAPYFAY), respectively. Additionally, the predicted nuclear export signal (NES) was shown to be nonfunctional. Taken together, this information opens up new avenues for investigating the biological functions of UL31 during PRV infection.