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Clinical Oral Investigations

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01.01.2013 | Original Article

Gingival fluid cytokine expression and subgingival bacterial counts during pregnancy and postpartum: a case series

verfasst von: Regina Alessandri Bieri, Laurence Adriaens, Stefan Spörri, Niklaus P. Lang, G. Rutger Persson

Erschienen in: Clinical Oral Investigations | Ausgabe 1/2013

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Abstract

Objectives

The aim of this study was to assess gingival fluid (GCF) cytokine messenger RNA (mRNA) levels, subgingival bacteria, and clinical periodontal conditions during a normal pregnancy to postpartum.

Materials and methods

Subgingival bacterial samples were analyzed with the checkerboard DNA–DNA hybridization method. GCF samples were assessed with real-time PCR including five proinflammatory cytokines and secretory leukocyte protease inhibitor.

Results

Nineteen pregnant women with a mean age of 32 years (S.D. ± 4 years, range 26–42) participated in the study. Full-mouth bleeding scores (BOP) decreased from an average of 41.2% (S.D. ± 18.6%) at the 12th week of pregnancy to 26.6% (S.D. ± 14.4%) at the 4–6 weeks postpartum (p < 0.001). Between week 12 and 4–6 weeks postpartum, the mean probing pocket depth changed from 2.4 mm (S.D. ± 0.4) to 2.3 mm (S.D. ± 0.3) (p = 0.34). Higher counts of Eubacterium saburreum, Parvimonas micra, Selenomonas noxia, and Staphylococcus aureus were found at week 12 of pregnancy than at the 4–6 weeks postpartum examinations (p < 0.001). During and after pregnancy, statistically significant correlations between BOP scores and bacterial counts were observed. BOP scores and GCF levels of selected cytokines were not related to each other and no differences in GCF levels of the cytokines were observed between samples from the 12th week of pregnancy to 4–6 weeks postpartum. Decreasing postpartum counts of Porphyromonas endodontalis and Pseudomonas aeruginosa were associated with decreasing levels of Il-8 and Il-1β.

Conclusions

BOP decreased after pregnancy without any active periodontal therapy. Associations between bacterial counts and cytokine levels varied greatly in pregnant women with gingivitis and a normal pregnancy outcome. Postpartum associations between GCF cytokines and bacterial counts were more consistent.

Clinical relevance

Combined assessments of gingival fluid cytokines and subgingival bacteria may provide important information on host response.

Loe H, Silness J (1963) Periodontal disease in pregnancy. I. Prevalence and severity. Acta Odontol Scand 21:533–551PubMedCrossRef

Kornman KS, Loesche WJ (1980) The subgingival microbial flora during pregnancy. J Periodontal Res 15:111–122PubMedCrossRef

Tilakaratne A, Soory M, Ranasinghe AW, Corea SM, Ekanayake SL, de Silva M (2000) Periodontal disease status during pregnancy and 3 months post-partum, in a rural population of Sri-Lankan women. J Clin Periodontol 27:787–792PubMedCrossRef

Gursoy M, Pajukanta R, Sorsa T, Kononen E (2008) Clinical changes in periodontium during pregnancy and post-partum. J Clin Periodontol 35:576–583PubMedCrossRef

Jensen J, Liljemark W, Bloomquist C (1981) The effect of female sex hormones on subgingival plaque. J Periodontol 52:599–602PubMedCrossRef

Carrillo-de-Albornoz A, Figuero E, Herrera D, Bascones-Martinez A (2010) Gingival changes during pregnancy: II. Influence of hormonal variations on the subgingival biofilm. J Clin Periodontol 37:230–240PubMedCrossRef

Jonsson R, Howland BE, Bowden GH (1988) Relationships between periodontal health, salivary steroids, and Bacteroides intermedius in males, pregnant and non-pregnant women. J Dent Res 67:1062–1069PubMedCrossRef

Adriaens LM, Alessandri R, Sporri S, Lang NP, Persson GR (2009) Does pregnancy have an impact on the subgingival microbiota? J Periodontol 80:72–81PubMedCrossRef

Ramseier CA, Kinney JS, Herr AE, Braun T, Sugai JV, Shelburne CA, Rayburn LA, Tran HM, Singh AK, Giannobile WV (2009) Identification of pathogen and host-response markers correlated with periodontal disease. J Periodontol 80:436–446PubMedCrossRef

10.

Teles RP, Likhari V, Socransky SS, Haffajee AD (2009) Salivary cytokine levels in subjects with chronic periodontitis and in periodontally healthy individuals: a cross-sectional study. J Periodontal Res 44:411–417PubMedCrossRef

11.

Bastos MF, Lima JA, Vieira PM, Mestnik MJ, Faveri M, Duarte PM (2009) TNF-alpha and IL-4 levels in generalized aggressive periodontitis subjects. Oral Dis 15:82–87PubMedCrossRef

12.

Markou E, Boura E, Tsalikis L, Deligianidis A, Konstantinidis A (2011) The influence of sex hormones on proinflammatory cytokines in gingiva of periodontally healthy premenopausal women. J Periodontal Res. doi:10.1111/j.1600-0765.2011.01369.x

13.

Figuero E, Carrillo-de-Albornoz A, Herrera D, Bascones-Martinez A (2010) Gingival changes during pregnancy: I. Influence of hormonal variations on clinical and immunological parameters. J Clin Periodontol 37:220–229PubMedCrossRef

14.

Into T, Inomata M, Kanno Y, Matsuyama T, Machigashira M, Izumi Y, Imamura T, Nakashima M, Noguchi T, Matsushita K (2006) Arginine-specific gingipains from Porphyromonas gingivalis deprive protective functions of secretory leucocyte protease inhibitor in periodontal tissue. Clin Exp Immunol 145:545–554PubMedCrossRef

15.

Weldon S, McGarry N, Taggart CC, McElvaney NG (2007) The role of secretory leucoprotease inhibitor in the resolution of inflammatory responses. Biochem Soc Trans 35(Pt 2):273–276PubMed

16.

Henriksen PA, Hitt M, Xing Z, Wang J, Haslett C, Riemersma RA, Webb DJ, Kotelevtsev YV, Sallenave JM (2004) Adenoviral gene delivery of elafin and secretory leukocyte protease inhibitor attenuates NF-kappa B-dependent inflammatory responses of human endothelial cells and macrophages to atherogenic stimuli. J Immunol 172:4535–4544PubMed

17.

Nakamura-Minami M, Furuichi Y, Ishikawa K, Mitsuzono-Tofuku Y, Izumi Y (2003) Changes of alpha1-protease inhibitor and secretory leukocyte protease inhibitor levels in gingival crevicular fluid before and after non-surgical periodontal treatment. Oral Dis 9:249–254PubMedCrossRef

18.

Sano C, Shimizu T, Sato K, Kawauchi H, Tomioka H (2000) Effects of secretory leucocyte protease inhibitor on the production of the anti-inflammatory cytokines, IL-10 and transforming growth factor-beta (TGF-beta), by lipopolysaccharide-stimulated macrophages. Clin Exp Immunol 121:77–85PubMedCrossRef

19.

Socransky SS, Haffajee AD, Smith C, Martin L, Haffajee JA, Uzel NG, Goodson JM (2004) Use of checkerboard DNA-DNA hybridization to study complex microbial ecosystems. Oral Microbiol Immunol 19:352–362PubMedCrossRef

20.

Persson GR, Weibel M, Hirschi R, Katsoulis J (2008) Similarities in the subgingival microbiota assessed by a curet sampling method at sites with chronic periodontitis. J Periodontol 79:2290–2296PubMedCrossRef

21.

Smith GL, Socransky SS, Smith CM (1989) Rapid method for the purification of DNA from subgingival microorganisms. Oral Microbiol Immunol 4:47–51PubMedCrossRef

22.

Novak MJ, Novak KF, Hodges JS, Kirakodu S, Govindaswami M, Diangelis A, Buchanan W, Papapanou PN, Michalowicz BS (2008) Periodontal bacterial profiles in pregnant women: response to treatment and associations with birth outcomes in the obstetrics and periodontal therapy (OPT) study. J Periodontol 79:1870–1879PubMedCrossRef

23.

Dinas K, Achyropoulos V, Hatzipantelis E, Mavromatidis G, Zepiridis L, Theodoridis T, Dovas D, Tantanasis T, Goutzioulis F, Bontis J (2007) Pregnancy and oral health: utilisation of dental services during pregnancy in northern Greece. Acta Obstet Gynecol Scand 86:938–944PubMedCrossRef

24.

Straka M (2011) Pregnancy and periodontal tissues. Neuro Endocrinol Lett 32:34–38PubMed

25.

26.

Gomes SC, Piccinin FB, Oppermann RV, Susin C, Nonnenmacher CI, Mutters R, Marcantonio RA (2006) Periodontal status in smokers and never-smokers: clinical findings and real-time polymerase chain reaction quantification of putative periodontal pathogens. J Periodontol 77:1483–1490PubMedCrossRef

27.

Fritschi BZ, Albert-Kiszely A, Persson GR (2008) Staphylococcus aureus and other bacteria in untreated periodontitis. J Dent Res 87:589–593PubMedCrossRef

28.

Cohen J (2002) The immunopathogenesis of sepsis. Nature 420:885–891PubMedCrossRef

29.

Raber-Durlacher JE, Leene W, Palmer-Bouva CC, Raber J, Abraham-Inpijn L (1993) Experimental gingivitis during pregnancy and post-partum: immunohistochemical aspects. J Periodontol 64:211–218PubMedCrossRef

30.

Ko HJ, Lim SS (2002) Production of macrophage inflammatory protein (MIP)-1alpha and MIP-1beta by human polymorphonuclear neutrophils stimulated with Porphyromonas endodontalis lipopolysaccharide. J Endod 28:754–757PubMedCrossRef

31.

Yin JL, Shackel NA, Zekry A, McGuinness PH, Richards C, Putten KV, McCaughan GW, Eris JM, Bishop GA (2001) Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green I. Immunol Cell Biol 79:213–221PubMedCrossRef

32.

Overbergh L, Giulietti A, Valckx D, Decallonne R, Bouillon R, Mathieu C (2003) The use of real-time reverse transcriptase PCR for the quantification of cytokine gene expression. J Biomol Tech 14:33–43PubMed

33.

King AE, Critchley HO, Kelly RW (2000) Presence of secretory leukocyte protease inhibitor in human endometrium and first trimester decidua suggests an antibacterial protective role. Mol Hum Reprod 6:191–196PubMedCrossRef

34.

da Silva-Boghossian CM, do Souto RM, Luiz RR, Colombo AP (2011) Association of red complex, A. actinomycetemcomitans and non-oral bacteria with periodontal diseases. Arch Oral Biol 56:899–906PubMedCrossRef

35.

Gamonal J, Acevedo A, Bascones A, Jorge O, Silva A (2000) Levels of interleukin-1 beta, -8, and -10 and RANTES in gingival crevicular fluid and cell populations in adult periodontitis patients and the effect of periodontal treatment. J Periodontol 71:1535–1545PubMedCrossRef

36.

Moffatt CE, Lamont RJ (2011) Porphyromonas gingivalis induction of microRNA-203 expression. Infect Immun 201179:2632–2637CrossRef

Titel: Gingival fluid cytokine expression and subgingival bacterial counts during pregnancy and postpartum: a case series
verfasst von: Regina Alessandri Bieri
Laurence Adriaens
Stefan Spörri
Niklaus P. Lang
G. Rutger Persson
Publikationsdatum: 01.01.2013
Verlag: Springer-Verlag
Erschienen in: Clinical Oral Investigations / Ausgabe 1/2013
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-012-0674-8

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Gingival fluid cytokine expression and subgingival bacterial counts during pregnancy and postpartum: a case series

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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