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Clinical Oral Investigations

Erschienen in:

06.06.2016 | Original Article

Comparison of periodontal ligament and gingiva-derived mesenchymal stem cells for regenerative therapies

verfasst von: Silvia Santamaría, Nerea Sanchez, Mariano Sanz, Jose A. Garcia-Sanz

Erschienen in: Clinical Oral Investigations | Ausgabe 4/2017

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Abstract

Objectives

Tissue-engineering therapies using undifferentiated mesenchymal cells (MSCs) from intra-oral origin have been tested in experimental animals. This experimental study compared the characteristics of undifferentiated mesenchymal stem cells from either periodontal ligament or gingival origin, aiming to establish the basis for the future use of these cells on regenerative therapies.

Materials and methods

Gingiva-derived mesenchymal stem cells (GMSCs) were obtained from de-epithelialized gingival biopsies, enzymatically digested and expanded in conditions of exponential growth. Their growth characteristics, phenotype, and differentiation ability were compared with those of periodontal ligament-derived mesenchymal stem cells (PDLMSCs).

Results

Both periodontal ligament- and gingiva-derived cells displayed a MSC-like phenotype and were able to differentiate into osteoblasts, chondroblasts, and adipocytes. These cells were genetically stable following in vitro expansion and did not generate tumors when implanted in immunocompromised mice. Furthermore, under suboptimal growth conditions, GMSCs proliferated with higher rates than PDLMSCs.

Conclusions

Stem cells derived from gingival biopsies represent bona fide MSCs and have demonstrated genetic stability and lack of tumorigenicity.

Clinical relevance

Gingiva-derived MSCs may represent an accessible source of messenchymal stem cells to be used in future periodontal regenerative therapies.

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Titel: Comparison of periodontal ligament and gingiva-derived mesenchymal stem cells for regenerative therapies
verfasst von: Silvia Santamaría
Nerea Sanchez
Mariano Sanz
Jose A. Garcia-Sanz
Publikationsdatum: 06.06.2016
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 4/2017
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-016-1867-3

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Comparison of periodontal ligament and gingiva-derived mesenchymal stem cells for regenerative therapies

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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