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Journal of Endocrinological Investigation

Erschienen in:

01.04.2017 | Original Article

Expression of mRNA and protein of IL-18 and its receptor in human follicular granulosa cells

verfasst von: A. Salmassi, A. Fattahi, M. Nouri, J. Hedderich, A. G. Schmutzler

Erschienen in: Journal of Endocrinological Investigation | Ausgabe 4/2017

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Abstract

Purpose

There is no information available about the IL-18 receptor in ovarian follicles, so the present study attempts to demonstrate the expression of IL-18 and its receptor in human granulosa cells (GCs).

Methods

To evaluate the concentration of IL-18 in serum and follicular fluid (FF), we collected serum and FF from 102 women undergoing oocyte retrieval. Also, to detect expression of IL-18 and its receptor by luteinized GCs, these cells were pooled six times from a total of twenty individual patients with 5–16 follicles each. The IL-18 concentration was determined by ELISA and the expression of IL-18 and its receptor by immunocytochemistry and reverse transcription polymerase chain reaction.

Results

Our results showed that the median IL-18 concentration in serum, 159.27 pg/ml (IQR 121.41–210.1), was significantly higher than in FF, 142.1 pg/ml (IQR 95.7–176.5), p < 0.001. Moreover, we found that IL-18 and its receptor are expressed by GCs.

Conclusion

The presence of IL-18 in FF and the expression of IL-18 and its receptor by GCs suggest an important role for this cytokine in ovarian function.

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Titel: Expression of mRNA and protein of IL-18 and its receptor in human follicular granulosa cells
verfasst von: A. Salmassi
A. Fattahi
M. Nouri
J. Hedderich
A. G. Schmutzler
Publikationsdatum: 01.04.2017
Verlag: Springer International Publishing
Erschienen in: Journal of Endocrinological Investigation / Ausgabe 4/2017
Elektronische ISSN: 1720-8386
DOI: https://doi.org/10.1007/s40618-016-0590-x

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