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01.02.2004 | Rapid Communication

Sustained cardiomyocyte apoptosis and left ventricular remodelling after myocardial infarction in experimental diabetes

verfasst von: T. Bäcklund, E. Palojoki, A. Saraste, A. Eriksson, P. Finckenberg, V. Kytö, P. Lakkisto, E. Mervaala, L.-M. Voipio-Pulkki, M. Laine, I. Tikkanen

Erschienen in: Diabetologia | Ausgabe 2/2004

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Abstract

Aims/hypothesis

Diabetes is known to reduce survival after myocardial infarction. Our aim was to examine whether diabetes is associated with enhanced cardiomyocyte apoptosis and thus interferes with the post-infarction remodelling process in myocardium in rat.

Methods

Four weeks after intravenous streptozotocin (diabetic groups) or citrate buffer (controls) injection, myocardial infarction was produced by ligation of left descending coronary artery. Level of cardiomyocyte apoptosis was quantified by TUNEL and caspase-3 methods. Collagen volume fraction and connective tissue growth factor were determined under microscope. Left ventricular dimensions were evaluated by echocardiography and planimetry.

Results

The number of apoptotic cardiomyocytes was equally high in diabetic and non-diabetic rats after 1 week from infarction. At 12 weeks after infarction the number of apoptotic cells was higher in the diabetic as compared to non-diabetic rats both in the border zone of infarction and in non-infarcted area. Correspondingly, left ventricular end diastolic diameter, relative cardiac weight, connective tissue growth factor-expression and fibrosis were increased in diabetic compared with non-diabetic rats with myocardial infarction.

Conclusion/interpretation

Sustained cardiomyocyte apoptosis, left ventricular enlargement, increased cardiac fibrosis and enhanced profibrogenic connective tissue growth factor expression were detected after myocardial infarction in experimental diabetes. Apoptotic myocyte loss could be an important mechanism contributing to progressive dilatation of the heart and poor prognosis after myocardial infarction in diabetes.

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Titel: Sustained cardiomyocyte apoptosis and left ventricular remodelling after myocardial infarction in experimental diabetes
verfasst von: T. Bäcklund
E. Palojoki
A. Saraste
A. Eriksson
P. Finckenberg
V. Kytö
P. Lakkisto
E. Mervaala
L.-M. Voipio-Pulkki
M. Laine
I. Tikkanen
Publikationsdatum: 01.02.2004
Verlag: Springer-Verlag
Erschienen in: Diabetologia / Ausgabe 2/2004
Print ISSN: 0012-186X
Elektronische ISSN: 1432-0428
DOI: https://doi.org/10.1007/s00125-003-1311-5

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