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01.03.2005 | Article

The mitochondrial rhomboid protease PSARL is a new candidate gene for type 2 diabetes

verfasst von: K. Walder, L. Kerr-Bayles, A. Civitarese, J. Jowett, J. Curran, K. Elliott, J. Trevaskis, N. Bishara, P. Zimmet, L. Mandarino, E. Ravussin, J. Blangero, A. Kissebah, G. R. Collier

Erschienen in: Diabetologia | Ausgabe 3/2005

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Abstract

Aims/hypothesis

This study aimed to identify genes that are expressed in skeletal muscle, encode proteins with functional significance in mitochondria, and are associated with type 2 diabetes.

Methods

We screened for differentially expressed genes in skeletal muscle of Psammomys obesus (Israeli sand rats), and prioritised these on the basis of genomic localisation and bioinformatics analysis for proteins with likely mitochondrial functions.

Results

We identified a mitochondrial intramembrane protease, known as presenilins-associated rhomboid-like protein (PSARL) that is associated with insulin resistance and type 2 diabetes. Expression of PSARL was reduced in skeletal muscle of diabetic Psammomys obesus, and restored after exercise training to successfully treat the diabetes. PSARL gene expression in human skeletal muscle was correlated with insulin sensitivity as assessed by glucose disposal during a hyperinsulinaemic–euglycaemic clamp. In 1,031 human subjects, an amino acid substitution (Leu262Val) in PSARL was associated with increased plasma insulin concentration, a key risk factor for diabetes. Furthermore, this variant interacted strongly with age to affect insulin levels, accounting for 5% of the variation in plasma insulin in elderly subjects.

Conclusions/interpretation

Variation in PSARL sequence and/or expression may be an important new risk factor for type 2 diabetes and other components of the metabolic syndrome.

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Titel: The mitochondrial rhomboid protease PSARL is a new candidate gene for type 2 diabetes
verfasst von: K. Walder
L. Kerr-Bayles
A. Civitarese
J. Jowett
J. Curran
K. Elliott
J. Trevaskis
N. Bishara
P. Zimmet
L. Mandarino
E. Ravussin
J. Blangero
A. Kissebah
G. R. Collier
Publikationsdatum: 01.03.2005
Verlag: Springer-Verlag
Erschienen in: Diabetologia / Ausgabe 3/2005
Print ISSN: 0012-186X
Elektronische ISSN: 1432-0428
DOI: https://doi.org/10.1007/s00125-005-1675-9

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