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International Urogynecology Journal

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01.09.2010 | Original Article

Tissue-engineered fascia from vaginal fibroblasts for patientsneeding reconstructive pelvic surgery

verfasst von: Man-Jung Hung, Mei-Chin Wen, Chia-Nung Hung, Esther Shih-Chu Ho, Gin-Den Chen, Vivian Cheng Yang

Erschienen in: International Urogynecology Journal | Ausgabe 9/2010

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Abstract

Introduction and hypothesis

Mesh-augmented reconstructive surgery for pelvic organ prolapse (POP) does not meet clinical expectations. A tissue-engineered fascia equivalent needs to be developed.

Methods

Human vaginal fibroblasts (HVFs) from 10 patients were characterized in vitro. Eligible HVFs and a biodegradable scaffold were used to fabricate a fascia equivalent, which was then transplanted in vivo.

Results

The cultured HVFs were divided into high (n = 6) or low (n = 4) collagen I/III ratio groups. Cells of the high-ratio group exhibited significantly higher proliferation potential than those of the low-ratio group (P < 0.05). A fascia equivalent was made with HVFs of the high-ratio group. In the subsequent animal study, a well-organized neo-fascia formation containing HVFs could be traced up to 12 weeks after transplantation.

Conclusions

Our results suggest that a tissue-engineered fascia could be developed from HVFs in vitro and in vivo, which might be an effective treatment for POP in the future.

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Titel: Tissue-engineered fascia from vaginal fibroblasts for patientsneeding reconstructive pelvic surgery
verfasst von: Man-Jung Hung
Mei-Chin Wen
Chia-Nung Hung
Esther Shih-Chu Ho
Gin-Den Chen
Vivian Cheng Yang
Publikationsdatum: 01.09.2010
Verlag: Springer-Verlag
Erschienen in: International Urogynecology Journal / Ausgabe 9/2010
Print ISSN: 0937-3462
Elektronische ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-010-1168-3

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Tissue-engineered fascia from vaginal fibroblasts for patientsneeding reconstructive pelvic surgery

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Introduction and hypothesis

Methods

Results

Conclusions

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Springer Medizin

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Introduction and hypothesis

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Results

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