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Urolithiasis

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01.08.2007 | Original Paper

Urinary 8-hydroxydeoxyguanosine is elevated in patients with nephrolithiasis

verfasst von: Chanchai Boonla, Rattiporn Wunsuwan, Kriang Tungsanga, Piyaratana Tosukhowong

Erschienen in: Urolithiasis | Ausgabe 4/2007

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Abstract

8-hydroxydeoxyguanosine (8-OHdG) is an oxidatively modified guanosine, which has been widely used as an oxidative DNA damage marker in various diseases. The present study aimed to determine urinary 8-OHdG in nephrolithiasis patients and evaluate its clinical significance. Thirty-six nephrolithiasis patients and 30 healthy subjects were recruited. Urine volume, creatinine, malondialdehyde, β-N-acetylglucosaminidase (NAG) activity and proteins were measured in 24 h urine samples. Urinary 8-OHdG was determined by competitive enzyme-linked immunosorbent assay. Mineral composition of stones was analyzed using Fourier-transformed infrared spectroscopy. Nephrolithiasis patients excreted urinary 8-OHdG significantly higher than healthy controls. Urinary 8-OHdG levels compared among patients with calcium oxalate, struvite and uric acid stones were insignificantly different. The urinary NAG activity correlated positively with urinary 8-OHdG. Multiple linear regression showed that urinary NAG activity was an independent predictor of urinary 8-OHdG level. Receiver operating characteristic analysis revealed that the urinary 8-OHdG test was adequate for diagnosing nephrolithiasis. At 10 μg/g creatinine cutoff, the 8-OHdG test imparted high specificity (96.67%) and a positive predictive value (91.67%). In conclusion, this is the first report of elevated urinary 8-OHdG excretion in nephrolithiasis patients indicating increased oxidative DNA damage. Increased renal tubular damage was independently associated with elevated urinary 8-OHdG. Elevated urinary 8-OHdG levels adjunct with metabolic profile may be useful for identifying people at risk of stone development.

Khan SR (2005) Hyperoxaluria-induced oxidative stress and antioxidants for renal protection. Urol Res 33:349–357PubMedCrossRef

Thamilselvan S, Khan SR, Menon M (2003) Oxalate and calcium oxalate mediated free radical toxicity in renal epithelial cells: effect of antioxidants. Urol Res 31:3–9PubMed

Umekawa T, Chegini N, Khan SR (2003) Increased expression of monocyte chemoattractant protein-1 (MCP-1) by renal epithelial cells in culture on exposure to calcium oxalate, phosphate and uric acid crystals. Nephrol Dial Transplant 18:664–669PubMedCrossRef

Huang MY, Chaturvedi LS, Koul S, Koul HK (2005) Oxalate stimulates IL-6 production in HK-2 cells, a line of human renal proximal tubular epithelial cells. Kidney Int 68:497–503PubMedCrossRef

Khan SR (2004) Crystal-induced inflammation of the kidneys:results from human studies, animal models, and tissue-culture studies. Clin Exp Nephrol 8:75–88PubMedCrossRef

Cooke MS, Evans MD, Dizdaroglu M, Lunec J (2003) Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J 17:1195–1214PubMedCrossRef

Wu LL, Chiou CC, Chang PY, Wu JT (2004) Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta 339:1–9PubMedCrossRef

Hata I, Kaji M, Hirano S, Shigematsu Y, Tsukahara H, Mayumi M (2006) Urinary oxidative stress markers in young patients with type 1 diabetes. Pediatr Int 48:58–61PubMedCrossRef

Erhola M, Toyokuni S, Okada K, Tanaka T, Hiai H, Ochi H, Uchida K, Osawa T, Nieminen MM, Alho H, Kellokumpu-Lehtinen P (1997) Biomarker evidence of DNA oxidation in lung cancer patients: association of urinary 8-hydroxy-2′-deoxyguanosine excretion with radiotherapy, chemotherapy, and response to treatment. FEBS Lett 409:287–291PubMedCrossRef

10.

Chiou CC, Chang PY, Chan EC, Wu TL, Tsao KC, Wu JT (2003) Urinary 8-hydroxydeoxyguanosine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers. Clin Chim Acta 334:87–94PubMedCrossRef

11.

Marshall PJ, Warso MA, Lands WE (1985) Selective microdetermination of lipid hydroperoxides. Anal Biochem 145:192–199PubMedCrossRef

12.

Horak E, Hopfer SM, Sunderman FW Jr (1981) Spectrophotometric assay for urinary N-acetyl-beta-D-glucosaminidase activity. Clin Chem 27:1180–1185PubMed

13.

Maxwell SR, Dietrich T, Chapple IL (2006) Prediction of serum total antioxidant activity from the concentration of individual serum antioxidants. Clin Chim Acta 372:188–194PubMedCrossRef

14.

Thamilselvan S, Khan SR (1998) Oxalate and calcium oxalate crystals are injurious to renal epithelial cells: results of in vivo and in vitro studies. J Nephrol 11(Suppl 1):66–69PubMed

15.

Huang HS, Ma MC, Chen CF, Chen J (2003) Lipid peroxidation and its correlations with urinary levels of oxalate, citric acid, and osteopontin in patients with renal calcium oxalate stones. Urology 62:1123–1128PubMedCrossRef

16.

Tungsanga K, Sriboonlue P, Futrakul P, Yachantha C, Tosukhowong P (2005) Renal tubular cell damage and oxidative stress in renal stone patients and the effect of potassium citrate treatment. Urol Res 33:65–69PubMedCrossRef

17.

Fraga CG, Shigenaga MK, Park JW, Degan P, Ames BN (1990) Oxidative damage to DNA during aging: 8-hydroxy-2′-deoxyguanosine in rat organ DNA and urine. Proc Natl Acad Sci USA 87:4533–4537PubMedCrossRef

18.

Park EM, Shigenaga MK, Degan P, Korn TS, Kitzler JW, Wehr CM, Kolachana P, Ames BN (1992) Assay of excised oxidative DNA lesions: isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column. Proc Natl Acad Sci USA 89:3375–3379PubMedCrossRef

19.

Faure H, Mousseau M, Cadet J, Guimier C, Tripier M, Hida H, Favier A (1998) Urine 8-oxo-7,8-dihydro-2-deoxyguanosine vs. 5-(hydroxymethyl) uracil as DNA oxidation marker in adriamycin-treated patients. Free Radic Res 28:377–382PubMedCrossRef

20.

Courtney PA, Crockard AD, Williamson K, Irvine AE, Kennedy RJ, Bell AL (1999) Increased apoptotic peripheral blood neutrophils in systemic lupus erythematosus: relations with disease activity, antibodies to double stranded DNA, and neutropenia. Ann Rheum Dis 58:309–314PubMedCrossRef

21.

Cooke MS, Evans MD, Dove R, Rozalski R, Gackowski D, Siomek A, Lunec J, Olinski R (2005) DNA repair is responsible for the presence of oxidatively damaged DNA lesions in urine. Mutat Res 574:58–66PubMed

22.

Evan A, Lingeman J, Coe FL, Worcester E (2006) Randall’s plaque: pathogenesis and role in calcium oxalate nephrolithiasis Kidney Int 69:1313–1318PubMedCrossRef

23.

Maki A, Kono H, Gupta M, Asakawa M, Suzuki T, Matsuda M, Fujii H, Rusyn I (2007) Predictive power of biomarkers of oxidative stress and inflammation in patients with hepatitis C virus-associated hepatocellular carcinoma. Ann Surg Oncol 14:1182–1190PubMedCrossRef

24.

Huang HS, Ma MC, Chen J, Chen CF (2002) Changes in the oxidant-antioxidant balance in the kidney of rats with nephrolithiasis induced by ethylene glycol. J Urol 167:2584–2593PubMedCrossRef

25.

Pilger A, Germadnik D, Riedel K, Meger-Kossien I, Scherer G, Rudiger HW (2001) Longitudinal study of urinary 8-hydroxy-2′-deoxyguanosine excretion in healthy adults. Free Radic Res 35:273–280PubMedCrossRef

26.

Kimura S, Yamauchi H, Hibino Y, Iwamoto M, Sera K, Ogino K (2006) Evaluation of urinary 8-hydroxydeoxyguanine in healthy Japanese people. Basic Clin Pharmacol Toxicol 98:496–502PubMedCrossRef

27.

Hakim IA, Harris RB, Brown S, Chow HH, Wiseman S, Agarwal S, Talbot W (2003) Effect of increased tea consumption on oxidative DNA damage among smokers: a randomized controlled study. J Nutr 133:3303S–3309SPubMed

28.

Besaratinia A, Van Schooten FJ, Schilderman PA, De Kok TM, Haenen GR, Van Herwijnen MH, Van Agen E, Pachen D, Kleinjans JC (2001) A multi-biomarker approach to study the effects of smoking on oxidative DNA damage and repair and antioxidative defense mechanisms. Carcinogenesis 22:395–401PubMedCrossRef

29.

Toraason M, Butler MA, Ruder A, Forrester C, Taylor L, Ashley DL, Mathias P, Marlow KL, Cheever KL, Krieg E, Wey H (2003) Effect of perchloroethylene, smoking, and race on oxidative DNA damage in female dry cleaners. Mutat Res 539:9–18PubMed

Titel: Urinary 8-hydroxydeoxyguanosine is elevated in patients with nephrolithiasis
verfasst von: Chanchai Boonla
Rattiporn Wunsuwan
Kriang Tungsanga
Piyaratana Tosukhowong
Publikationsdatum: 01.08.2007
Verlag: Springer-Verlag
Erschienen in: Urolithiasis / Ausgabe 4/2007
Print ISSN: 2194-7228
Elektronische ISSN: 2194-7236
DOI: https://doi.org/10.1007/s00240-007-0098-0

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