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Urolithiasis
Erschienen in: Urolithiasis 6/2007

01.12.2007 | Original Paper

Reduction in oxalate-induced renal tubular epithelial cell injury by an extract from Quercus salicina Blume/Quercus stenophylla Makino

verfasst von: Manabu T. Moriyama, Katsuhito Miyazawa, Kumiko Noda, Michiko Oka, Mitsushi Tanaka, Koji Suzuki

Erschienen in: Urolithiasis | Ausgabe 6/2007

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Abstract

Urocalun®, a herbal medicine prepared from an extract of Quercus salicina Blume/Quercus stenophylla Makino (QS extract), has been clinically used for the treatment of urolithiasis in Japan since 1969. In the present study, the effects of QS extract on oxalate-induced cell injury and NADPH-induced superoxide anion (O2 −) production in the injured cells were investigated. Oxalate-induced cell injury was assessed by mitochondrial reduction of 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyltetrazolium bromide and leakage of lactate dehydrogenase into the extracellular fluid. When NRK-52E cells were injured by exposure to oxalate for 24 h, QS extract prevented the injury in a dose-dependent manner. In addition, QS extract suppressed the increase in NADPH-induced O2 − production, or NADPH oxidase activity, in the homogenate of cells injured by oxalate exposure. These findings suggest that the reduction in oxalate-induced O2 − production contributes to the cytoprotective effect of QS extract.
Literatur
1.
Finlayson B (1974) Symposium on renal lithiasis. Renal lithiasis in review. Urol Clin North Am 1:181–212PubMed
2.
Wahl C, Hess A (2000) Kidney calculi–is nutrition a trigger or treatment? Ther Umsch 57:138–145PubMedCrossRef
3.
Natsukawa T, Hirata K, Noda K, Oka M, Tanaka M, Hamada K, Kuwabara T (2005) Promoting effect of extract of Quercus salicina Blume/Q. stenophylla Makino (UROCALUN®) on urinary stone passage. Jpn Pharmacol Ther 33:361–368
4.
Ishigami J, Ooshima H, Saitou H (1967) Clinical effect of UROCALUN on urolithiasis in the upper urinary tract. Hifu to Hinyo 29:858–862
5.
Ookita K, Yamada S, Johsen T, Tanaka M (1968) Uses of UC-2 for treatment of upper urinary calculi and studies on dissolution of urinary calculi by UC-2. Hifu to Hinyo 30:426–431
6.
Huang HS, Ma MC, Chen J, Chen CF (2002) Changes in the oxidant–antioxidant balance in the kidney of rats with nephrolithiasis induced by ethylene glycol. J Urol 167:2584–2593PubMedCrossRef
7.
Selvam R (2002) Calcium oxalate stone disease: role of lipid peroxidation and antioxidants. Urol Res 30:35–47PubMedCrossRef
8.
Thamilselvan S (1998) Oxalate and calcium oxalate crystals are injurious to renal epithelial cells: results of in vivo and in vitro studies. J Nephrol 11(Suppl 1):66–69PubMed
9.
Wiessner JH, Hasegawa AT, Hung LY, Mandel GS, Mandel NS (2001) Mechanisms of calcium oxalate crystal attachment to injured renal collecting duct cells. Kidney Int 59:637–644PubMedCrossRef
10.
Khan SR (1995) Calcium oxalate crystal interaction with renal tubular epithelium, mechanism of crystal adhesion and its impact on stone development. Urol Res 23:71–79PubMedCrossRef
11.
Rashed T, Menon M, Thamilselvan S (2004) Molecular mechanism of oxalate-induced free radical production and glutathione redox imbalance in renal epithelial cells: effect of antioxidants. Am J Nephrol 24:557–568PubMedCrossRef
12.
Thamilselvan S, Byer KJ, Hackett RL, Khan SR (2000) Free radical scavengers, catalase and superoxide dismutase provide protection from oxalate-associated injury to LLC-PK1 and MDCK cells. J Urol 164:224–229PubMedCrossRef
13.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254PubMedCrossRef
14.
Scheid C, Koul H, Hill WA, Luber-Narod J, Kennington L, Honeyman T, Jonassen J, Menon M (1996) Oxalate toxicity in LLC-PK1 cells: role of free radicals. Kidney Int 49:413–419PubMedCrossRef
15.
Thamilselvan S, Khan SR, Menon M (2003) Oxalate and calcium oxalate mediated free radical toxicity in renal epithelial cells: effect of antioxidants. Urol Res 31:3–9PubMed
16.
Hackett RL, Shevock PN, Khan SR (1990) Cell injury associated calcium oxalate crystalluuria. J Urol 144:1535–1538PubMed
17.
Ebisuno S, Kohjimoto Y, Tamura M, Ohkawa T (1995) Adhesion of calcium oxalate crystal to Madin-Darby canine kidney cells and some effects of glycosaminoglycans or cell injuries. Eur Urol 28:68–73PubMed
18.
Schepers MS, van Ballegooijen ES, Bangma CH, Verkoelen CF (2005) Crystals cause acute necrotic cell death in renal proximal tubule cells, but not in collecting tubule cells. Kidney Int 68:1543–1553PubMedCrossRef
19.
Schepers MS, van Ballegooijen ES, Bangma CH, Verkoelen CF (2005) Oxalate is toxic to renal tubular cells only at supraphysiologic concentrations. Kidney Int 68:1660–1669PubMedCrossRef
20.
Miyazawa K, Suzuki K, Ikeda R, Moriyama MT, Ueda Y, Katsuda S (2005) Apoptosis and its related genes in renal epithelial cells of the stone-forming rat. Urol Res 33:31–38PubMedCrossRef
21.
Sarica K, Yagci F, Bakir K, Erbagci A, Erturhan S, Ucak R (2001) Renal tubular injury induced by hyperoxaluria: evaluation of apoptotic changes. Urol Res 29:34–37PubMedCrossRef
22.
Huang HS, Ma MC, Chen CF, Chen J (2003) Lipid peroxidation and its correlations with urinary levels of oxalate, citric acid, and osteopontin in patients with renal calcium oxalate stones. Urology 62:1123–1128PubMedCrossRef
23.
Thamilselvan S, Menon M (2005) Vitamin E therapy prevents hyperoxaluria-induced calcium oxalate crystal deposition in the kidney by improving renal tissue antioxidant. BJU Int 96:117–126PubMedCrossRef
24.
Byer K, Khan SR (2005) Citrate provides protection against oxalate and calcium oxalate crystal induced oxidative damage to renal epithelium. J Urol 173:640–646PubMedCrossRef
25.
Coe FL, Parks JH, Asplin JR (1992) The pathogenesis and treatment of kidney stones. N Engl J Med 327:1141–1152PubMedCrossRef
26.
Moriyama MT, Domiki C, Miyazawa K, Tanaka T, Suzuki K (2005) Effect of oxalate exposure on Madin-Darby canine kidney (MDCK) cells in culture. Urol Res 33:470–475CrossRef
27.
Umekawa T, Byer K, Uemura H, Khan SR (2005) Diphenyleneiodium (DPI) reduces oxalate ion- and calcium oxalate monohydrate and brushite crystal-induced upregulation of MCP-1 in NRK52E cells. Nephrol Dial Transplant 20:870–878PubMedCrossRef
28.
Atmani F, Farell G, Lieske JC (2004) Extract from Herniaria hirsuta coats calcium oxalate monohydrate crystals and blocks their adhesion to renal epithelial cells. J Urol 172:1510–1514PubMedCrossRef
29.
Campos AH, Schor N (1999) Phyllanthus niruri inhibits calcium oxalate endocytosis by renal tubular cells: its role in urolithiasi. Nephron 81:393–397PubMedCrossRef
30.
Srinivasan S, Pragasam V, Jenita X, Kalaiselvi P, Muthu V, Varalakshmi P (2004) Oxidative stress in urogenital tuberculosis patients: a predisposing factor for renal stone formation–amelioration by vitamin E supplementation. Clin Chim Acta 350:57–63PubMedCrossRef
Metadaten
Titel
Reduction in oxalate-induced renal tubular epithelial cell injury by an extract from Quercus salicina Blume/Quercus stenophylla Makino
verfasst von
Manabu T. Moriyama
Katsuhito Miyazawa
Kumiko Noda
Michiko Oka
Mitsushi Tanaka
Koji Suzuki
Publikationsdatum
01.12.2007
Verlag
Springer-Verlag
Erschienen in
Urolithiasis / Ausgabe 6/2007
Print ISSN: 2194-7228
Elektronische ISSN: 2194-7236
DOI
https://doi.org/10.1007/s00240-007-0114-4

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