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01.11.2011 | Original Article

Labeling human embryonic stem-cell-derived cardiomyocytes for tracking with MR imaging

verfasst von: Rosalinda T. Castaneda, Sophie Boddington, Tobias D. Henning, Mike Wendland, Lydia Mandrussow, Siyuan Liu, Heike Daldrup-Link

Erschienen in: Pediatric Radiology | Ausgabe 11/2011

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Abstract

Background

Human embryonic stem cells (hESC) can generate cardiomyocytes (CM), which offer promising treatments for cardiomyopathies in children. However, challenges for clinical translation result from loss of transplanted cell from target sites and high cell death. An imaging technique that noninvasively and repetitively monitors transplanted hESC-CM could guide improvements in transplantation techniques and advance therapies.

Objective

To develop a clinically applicable labeling technique for hESC-CM with FDA-approved superparamagnetic iron oxide nanoparticles (SPIO) by examining labeling before and after CM differentiation.

Materials and methods

Triplicates of hESC were labeled by simple incubation with 50 μg/ml of ferumoxides before or after differentiation into CM, then imaged on a 7T MR scanner using a T2-weighted multi-echo spin-echo sequence. Viability, iron uptake and T2-relaxation times were compared between groups using t-tests.

Results

hESC-CM labeled before differentiation demonstrated significant MR effects, iron uptake and preserved function. hESC-CM labeled after differentiation showed no significant iron uptake or change in MR signal (P < 0.05). Morphology, differentiation and viability were consistent between experimental groups.

Conclusion

hESC-CM should be labeled prior to CM differentiation to achieve a significant MR signal. This technique permits monitoring delivery and engraftment of hESC-CM for potential advancements of stem cell-based therapies in the reconstitution of damaged myocardium.

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Titel: Labeling human embryonic stem-cell-derived cardiomyocytes for tracking with MR imaging
verfasst von: Rosalinda T. Castaneda
Sophie Boddington
Tobias D. Henning
Mike Wendland
Lydia Mandrussow
Siyuan Liu
Heike Daldrup-Link
Publikationsdatum: 01.11.2011
Verlag: Springer-Verlag
Erschienen in: Pediatric Radiology / Ausgabe 11/2011
Print ISSN: 0301-0449
Elektronische ISSN: 1432-1998
DOI: https://doi.org/10.1007/s00247-011-2130-3

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Labeling human embryonic stem-cell-derived cardiomyocytes for tracking with MR imaging