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Cancer Immunology, Immunotherapy

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01.05.2010 | Original Paper

Topoisomerase II alpha as a universal tumor antigen: antitumor immunity in murine tumor models and H-2K^b-restricted T cell epitope

verfasst von: Jung-Sun Park, Hye-Sung Kim, Mi-Young Park, Chang-Hyun Kim, Yeun-Jun Chung, Yong-Kil Hong, Tai-Gyu Kim

Erschienen in: Cancer Immunology, Immunotherapy | Ausgabe 5/2010

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Abstract

Topoisomerase II alpha (Top2α) is an attractive candidate to be used as a tumor antigen for cancer immunotherapy, because it is abundantly expressed in various tumors and serves as a target for a number of chemotherapeutic agents. In this study, we demonstrated the immunogenicity of Top2α, using dendritic cells (DC) electroporated with RNA encoding the Top2α C-terminus (Top2αCRNA/DC). Top2αCRNA/DC were able to demonstrate in vitro stimulation of T cells from mice that were previously vaccinated with Top2α-expressing tumor lysate-pulsed DC. Vaccination with Top2αCRNA/DC induced Top2α-specific T cell responses in vivo as well as antitumor effects in various murine tumor models including MC-38, B16F10, and GL26. DC pulsed with p1327 (DSDEDFSGL), defined as an epitope presented by H-2K^b, also induced Top2α-specific immune responses and antitumor effects. Based on these data, Top2α is suggested to be a universal target for cancer immunotherapy.

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Titel: Topoisomerase II alpha as a universal tumor antigen: antitumor immunity in murine tumor models and H-2Kb-restricted T cell epitope
verfasst von: Jung-Sun Park
Hye-Sung Kim
Mi-Young Park
Chang-Hyun Kim
Yeun-Jun Chung
Yong-Kil Hong
Tai-Gyu Kim
Publikationsdatum: 01.05.2010
Verlag: Springer-Verlag
Erschienen in: Cancer Immunology, Immunotherapy / Ausgabe 5/2010
Print ISSN: 0340-7004
Elektronische ISSN: 1432-0851
DOI: https://doi.org/10.1007/s00262-009-0795-3

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Springer Medizin

Topoisomerase II alpha as a universal tumor antigen: antitumor immunity in murine tumor models and H-2K^b-restricted T cell epitope

Abstract

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