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01.04.2008 | Original Article

Protective effects of sildenafil administration on testicular torsion/detorsion damage in rats

verfasst von: Azadeh Beheshtian, Amirali Hassanzadeh Salmasi, Seyedmehdi Payabvash, Saman Kiumehr, Behtash Ghazinezami, Sina Rahimpour, Seyed Mohammad Tavangar, Ahmad Reza Dehpour

Erschienen in: World Journal of Urology | Ausgabe 2/2008

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Abstract

Purpose

We assessed the effectiveness of sildenafil administration during ischemic period in a rat model of testicular torsion/detorsion (T/D).

Material and methods

Sprague–Dawley rats were divided into four groups (n = 10). In those animals that underwent T/D, right testes were rotated 720° for 1 h. Base line group was for basal normal values. Sham operated group was served as a control group. T/D group underwent 1 h testicular torsion. Sildenafil group received sildenafil (0.7 mg/kg) intraperitoneally 30 min after initiation of ischemic period. For measurement of lipid peroxidation and antioxidant enzyme activities, right testes of five animals in each group were excised after 4-h reperfusion. Germ cell apoptosis indices were determined 24 h following detorsion in right testes of remaining five animals in each group.

Results

Malondialdehyde (MDA) levels in T/D group were significantly higher versus control and base line groups. Moreover, testicular MDA values in sildenafil group were significantly lower than T/D. There were also significant decreases in catalase and superxide dismutase activities in T/D group compared with control and base line groups. These values were significantly higher in sildenafil group versus T/D. Germ cell apoptosis indices were significantly higher in both groups that experienced T/D in comparison to control and base line groups; however, sildenafil treatment significantly reduced the apoptosis in sildenafil group compared with T/D group.

Conclusion

Sildenafil administration during testicular torsion decreased ischemia/reperfusion cellular damage. The results of biochemical studies suggest that, reduction of oxidative stress by sildenafil may have a major role in its cytoprotective effects.

Williamson RC (1985) The continuing conundrum of testicular torsion. Br J Surg 72:509–510PubMedCrossRef

Lysiak JJ, Nguyen QA, Turner TT (2002) Peptide and nonpeptide reactive oxygen scavengers provide partial rescue of the testis after torsion. J Androl 23:400–409PubMed

Corbin JD, Francis SH (2002) Pharmacology of phosphodiesterase-5 inhibitors. Int J Clin Pract 56:453–459PubMed

Kukreja RC, Salloum F, Das A, Ockaili R, Yin C, Bremer YA, Fisher PW, Wittkamp M, Hawkins J, Chou E, Kukreja AK, Wang X, Marwaha VR, Xi L (2005) Pharmacological preconditioning with sildenafil: Basic mechanisms and clinical implications. Vascul Pharmacol 42:219–232PubMedCrossRef

Li J, Fu P, Deleon M, French BA, French SW (2005) The effect of Viagra (sildenafil citrate) on liver injury caused by chronic ethanol intragastric feeding in rats. Exp Mol Pathol 78:101–108PubMedCrossRef

Zhang RL, Zhang Z, Zhang L, Wang Y, Zhang C, Chopp M (2006) Delayed treatment with sildenafil enhances neurogenesis and improves functional recovery in aged rats after focal cerebral ischemia. J Neurosci Res 1:1213–1219CrossRef

Muzaffar S, Shukla N, Srivastava A, Angelini GD, Jeremy JY (2005) Sildenafil citrate and sildenafil nitrate (NCX 911) are potent inhibitors of superoxide formation and gp91phox expression in porcine pulmonary artery endothelial cells. Br J Pharmacol 146:109–117PubMedCrossRef

Koupparis AJ, Jeremy JY, Muzaffar S, Persad R, Shukla N (2005) Sildenafil inhibits the formation of superoxide and the expression of gp47 NAD[P]H oxidase induced by the thromboxane A2 mimetic, U46619, in corpus cavernosal smooth muscle cells. BJU Int 96:423–427PubMedCrossRef

Chanani NK, Cowan DB, Takeuchi K, Poutias DN, Garcia LM, Del Nido PJ, McGowan FXJ (2002) Differential effects of amrinone and milrinone upon myocardial inflammatory signaling. Circulation 24:1284–1289

10.

Toward TJ, Smith N, Broadley KJ (2004) Effect of phosphodiesterase-5 inhibitor, sildenafil (Viagra), in animal models of airways disease. Am J Respir Crit Care Med 169:227–234PubMedCrossRef

11.

Ockaili R, Salloum F, Hawkins J, Kukreja RC (2002) Sildenafil (Viagra) induces powerful cardioprotective effect via opening of mitochondrial K(ATP) channels in rabbits. Am J Physiol Heart Circ Physiol 283:1263–1269

12.

Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358PubMedCrossRef

13.

Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126PubMedCrossRef

14.

Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169PubMed

15.

Paoletti F, Mocali A (1990) Determination of superoxide dismutase activity by purely chemical system based on NAD(P)H oxidation. Methods Enzymol 186:209–220PubMed

16.

Anderson JB, Williamson RC (1986) The fate of the human testes following unilateral torsion of the spermatic cord. Br J Urol 58:698–704PubMed

17.

Turner TT, Bang HJ, Lysiak JL (2004) The molecular pathology of experimental testicular torsion suggests adjunct therapy to surgical repair. J Urol 172:2574–2578PubMedCrossRef

18.

Salmasi AH, Beheshtian A, Payabvash S, Demehri S, Ebrahimkhani MR, Karimzadegan M, Bahadori M, Pasalar P, Dehpour AR (2005) Effect of morphine on ischemia-reperfusion injury: experimental study in testicular torsion rat model. Urology 66:1338–1342PubMedCrossRef

19.

Filho DW, Torres MA, Bordin AL, Crezcynski-Pasa TB, Boveris A (2004) Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia-reperfusion injury. Mol Aspects Med 25:199–210PubMedCrossRef

20.

Ardehali H, O’Rourke B (2005) Mitochondrial K(ATP) channels in cell survival and death. J Mol Cell Cardiol 39:7–16PubMedCrossRef

21.

Kitakaze M, Hori M, Gotoh K, Sato H, Iwakura K, Kitabatake A, Inoue M, Kamada T (1989) Beneficial effects of alpha 2-adrenoceptor activity on ischemic myocardium during coronary hypoperfusion in dogs. Circ Res 65:1632–45PubMed

22.

Chin S, Ikeda M, Ozaki M, Kameoka S (2005) Protective effect of diltiazem on hepatic ischemia-reperfusion injury in rats by improving liver tissue blood flow. Transplant Proc 37:4556–9PubMedCrossRef

23.

Savas C, Dindar H, Bilgehan A, Ataoglu O, Yucesan S (2002) Pentoxifylline attenuates reperfusion injury in testicular torsion. Scand J Urol Nephrol 36:65–70PubMedCrossRef

24.

Savas C, Dindar H, Aras T, Yucesan S (2002) Pentoxifylline improves blood flow to both testes in testicular torsion. Int Urol Nephrol 33:81–5PubMedCrossRef

25.

Halcox JP, Nour KR, Zalos G, Mincemoyer RA, Waclawiw M, Rivera CE, Willie G, Ellahham S, Quyyumi AA (2002) The effect of sildenafil on human vascular function, platelet activation, and myocardial ischemia. J Am Coll Cardiol 40:1232–1240PubMedCrossRef

Titel: Protective effects of sildenafil administration on testicular torsion/detorsion damage in rats
verfasst von: Azadeh Beheshtian
Amirali Hassanzadeh Salmasi
Seyedmehdi Payabvash
Saman Kiumehr
Behtash Ghazinezami
Sina Rahimpour
Seyed Mohammad Tavangar
Ahmad Reza Dehpour
Publikationsdatum: 01.04.2008
Verlag: Springer-Verlag
Erschienen in: World Journal of Urology / Ausgabe 2/2008
Print ISSN: 0724-4983
Elektronische ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-008-0243-6

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Protective effects of sildenafil administration on testicular torsion/detorsion damage in rats