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World Journal of Urology

Erschienen in:

01.11.2015 | Original Article

Magnitude-dependent proliferation and contractility modulation of human bladder smooth muscle cells under physiological stretch

verfasst von: De-Yi Luo, Romel Wazir, Caigan Du, Ye Tian, Xuan Yue, Tang-Qiang Wei, Kun-Jie Wang

Erschienen in: World Journal of Urology | Ausgabe 11/2015

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Abstract

Purpose

The purpose of this study was to describe and test a kind of stretch pattern which is based on modified BOSE BioDynamic system to produce optimum physiological stretch during bladder cycle. Moreover, we aimed to emphasize the effects of physiological stretch’s amplitude upon proliferation and contractility of human bladder smooth muscle cells (HBSMCs).

Methods

HBSMCs were seeded onto silicone membrane and subjected to stretch simulating bladder cycle at the range of stretches and time according to customized software on modified BOSE BioDynamic bioreactor. Morphological changes were assessed using immunofluorescence and confocal laser scanning microscope. Cell proliferation and cell viability were determined by BrdU incorporation assay and Cell Counting Kit-8, respectively. Contractility of the cells was determined using collagen gel contraction assay. RT-PCR was used to assess phenotypic and contractility markers.

Results

HBSMCs were found to show morphologically spindle-shaped and orientation at various elongations in the modified bioreactor. Stretch-induced proliferation and viability depended on the magnitude of stretch, and stretches also regulate contractility and contraction markers in a magnitude-dependent manner.

Conclusion

We described and tested a kind of stretch pattern which delivers physiological stretch implemented during bladder cycle. The findings also showed that mechanical stretch can promote magnitude-dependent morphological, proliferative and contractile modulation of HBSMCs in vitro.

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Titel: Magnitude-dependent proliferation and contractility modulation of human bladder smooth muscle cells under physiological stretch
verfasst von: De-Yi Luo
Romel Wazir
Caigan Du
Ye Tian
Xuan Yue
Tang-Qiang Wei
Kun-Jie Wang
Publikationsdatum: 01.11.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: World Journal of Urology / Ausgabe 11/2015
Print ISSN: 0724-4983
Elektronische ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-015-1509-4

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