Introduction
Materials and methods
Subject selection
Leptomeningeal sample selection
Sample fixing, staining and immunohistochemistry
Examination of samples
Analysis of results
Results
Demographics
Case no | General autopsy findings | Neuropathology findings |
---|---|---|
1 | Chronic aspiration/pneumonitis with MOSF | Oedema with herniation; HIE |
Seizure disorder | ||
4 | Noonan’s syndrome | Micrencephaly; pontosubicular neuronal necrosis |
5 | Trisomy 21 with CHD | Haemorrhagic infarct (left periventricular) |
7 | CHD | Micrencephaly |
8 | Interstitial pneumonitis (cultures negative) | PVL |
9 | Heterotaxy–asplenia syndrome | Micrencephaly; multiple cortical and WM infarcts |
11 | Congenital pulmonary malformation | Multifocal acute HI changes |
12 | Pulmonary hypoplasia | Remote and focal acute HI changes |
14 | None | Agenesis of CC |
(Brain only) | ||
17 | CHD with infarction | None |
22 | Cardiomyopathy | Multiple cerebral infarctions |
Sepsis with MOSF | ||
23 | Foetal hydrops | PVL; right occipital infarction |
26 | Noonan’s syndrome | Micrencephaly; hypomyelination; organising SAH, SDH |
27 | Liver failure | Metabolic astrogliosis; neuronal pyknosis; bilateral subdural membranes |
28 | RSV bronchiolitis | Remote occipital infarct; subacute EDH |
PNA | ||
33 | CHD | Cystic infarct (frontal) |
Case no. | General autopsy findings | Neuropathologic findings |
---|---|---|
2 | CHD | None |
3 | CHD | Micrencephaly; |
Arhinencephaly; | ||
Neuronal necrosis, subiculum | ||
6 | Harlequin ichthyosis | None |
10 | Diaphragmatic hernia Omphalocoele | PSNN |
13 | PNA (Strep) | Metabolic encephalopathy; |
Hydrocephalus; | ||
Partial agenesis of CC | ||
15 | CHD | Thoracic meningomyelocoele; |
Hydrocephalus; possible partial agenesis of CC | ||
16 | CHD | Organising SDH |
18 | Cardiomyopathy | Acute HI change (focal); |
DiGeorge syndrome | PSNN; | |
Remote dural haemorrhage | ||
19 | Pulmonary HTN | PSNN |
20 | Foetal hydrops | GM haemorrhage extending to SA; |
Diffuse HIE | ||
21 | Tetralogy of Fallot | PSNN; BG infarct |
24 | CHD | Focal SAH; |
Small WM haemorrhages; | ||
PSNN | ||
25 | CHD | PSNN; |
Focal HI, cerebellum; | ||
PVL; | ||
Parietal infarct; | ||
Organising SDH, IVH, SAH | ||
29 | None (brain only) | Diffuse HIE |
30 | NEC (MOSF) | Meningoencephalitis; |
PSNN | ||
31 | Multiple congenital abnormalities including CHD | GM haemorrhage with IVH and SAH; PSNN; |
Arhinencephaly | ||
32 | Pulmonary hypoplasia/HTN | GM haemorrhage with IV and SAH; PSNN |
Sites | No. of sites sampled | Leptomeningeal length (mm) | Mean (mm) +/− SD |
---|---|---|---|
Cerebral cortex | 39 | 5−41 | 18.8 +/− 10.5 |
Brain stem | 37 | 10−83 | 28.7 +/− 17.0 |
Cerebellum | 30 | 10−47 | 20.3 +/− 11.1 |
Comparison (group 1 vs. group 2) |
P-value | |
---|---|---|
Cerebellum | CD45 | 0.623 |
CD68 | 0.235 | |
CD163 | 0.457 | |
Brain stem | CD45 | 0.689 |
CD68 | 0.337 | |
CD163 | 0.172 | |
Cerebral cortex | CD45 | 0.247 |
CD68 | 0.149 | |
CD163 | 0.204 |