nach oben

Erschienen in:

01.02.2014 | Maternal-Fetal Medicine

Oocyte morphology on day 0 correlates with aneuploidy as detected by polar body biopsy and FISH

verfasst von: Andreas G. Schmutzler, Bengi Acar-Perk, Jörg Weimer, Ali Salmassi, Knud Sievers, Monica Tobler, Liselotte Mettler, Walter Jonat, Norbert Arnold

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 2/2014

Einloggen, um Zugang zu erhalten

Abstract

Purpose

For better selection of oocytes and embryos, preimplantation genetic screening (PGS) was introduced. As from the beginning of IVF, morphology was used as selection criteria; we investigated the combination of both. If there was a correlation between phenotype and genotype, invasive PGS might be replaced.

Method

Therefore, 104 cycles with PGS were done by biopsy of the first polar body and FISH with five chromosomes. Morphology of the oocyte was recorded digitally and noted for 12 categories in 4–13 values; evaluation of the chromosomes was noted for five chromosomes in five values. Morphology and genetics were correlated to each other.

Result

Correlations between morphology and genetics for day 0 were found: oocytes with an irregular or dark zona are less probable to have a normal chromosome 13 (80 vs. 53 %, p = 0.001). A medium amount of detritus in the perivitelline space makes it more probable to have a normal chromosome 18 (94 vs. 78 %, p = 0.001). A halo in the cytoplasm makes it less probable to be euploid for chromosome 22 (56 vs. 75 %, p = 0.018). For day 1, pattern “1, 2, 3 and fine” in the pronuclei makes it more probable to be euploid for chromosome 22 (78 vs. 63 %, p = 0.002).

Conclusion

There are correlations between the oocyte genome and its morphology also on day 0. These correlations are not sufficient to replace PGS.

Geraedts J, Collins J, Gianaroli L, Goossens V, Handyside A, Harper J, Montag M, Repping S, Schmutzler A (2010) What next for preimplantation genetic screening? A polar body approach! Hum Reprod 25:575–577PubMedCrossRef

Schoolcraft, W. B., Surrey, E., Minjarez, D., Gustofson, R. L., Scott, J., R T and Katz-Jaffe, M. G. (2012) Comprehensive chromosome screening (CCS) with vitrification results in improved clinical outcome in women >35 years: a randomized control trial. Fertil Steril, 98, Suppl 1

Yang Z, Liu J, Collins GS, Salem SA, Liu X, Lyle SS, Peck AC, Sills ES, Salem RD (2012) Selection of single blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good prognosis IVF patients: results from a randomized pilot study. Mol Cytogenet 5:24PubMedCentralPubMedCrossRef

Rubio C, Bellver J, Rodrigo L, Bosch E, Mercader A, Vidal C, De Los Santos MJ, Giles J, Labarta E, Domingo J, Crespo J, Remohi J, Pellicer A, Simon C (2013) Preimplantation genetic screening using fluorescence in situ hybridization in patients with repetitive implantation failure and advanced maternal age: two randomized trials. Fertil Steril 99:1400–1407PubMedCrossRef

Schmutzler A, Acar-Perk B, Weimer J, Salmassi A, Sievers K, Tobler M, Mettler L, Jonat W, Arnold N (2013) Sham controlled implantation after preimplantation genetic screening by polar body biopsy and FISH. Arch Gynecol Obstet. doi:10.1007/s00404-013-2945-2

Van Blerkom J, Henry G (1992) Oocyte dysmorphism and aneuploidy in meiotically mature human oocytes after ovarian stimulation. Hum Reprod 7:379–390PubMed

Kahraman S, Kumtepe Y, Sertyel S, Donmez E, Benkhalifa M, Findikli N, Vanderzwalmen P (2002) Pronuclear morphology scoring and chromosomal status of embryos in severe male infertility. Hum Reprod 17:3193–3200PubMedCrossRef

Balaban B, Yakin K, Urman B, Isiklar A, Tesarik J (2004) Pronuclear morphology predicts embryo development and chromosome constitution. Reprod Biomed Online 8:695–700PubMedCrossRef

Gianaroli L, Magli MC, Ferraretti AP, Fortini D, Grieco N (2003) Pronuclear morphology and chromosomal abnormalities as scoring criteria for embryo selection. Fertil Steril 80:341–349PubMedCrossRef

10.

Gianaroli L, Magli MC, Ferraretti AP, Lappi M, Borghi E, Ermini B (2007) Oocyte euploidy, pronuclear zygote morphology and embryo chromosomal complement. Hum Reprod 22:241–249PubMedCrossRef

11.

Campbell A, Fishel S, Bowman N, Duffy S, Sedler M, Hickman CF (2013) Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics. Reprod Biomed Online 26:477–485PubMedCrossRef

12.

Glander, M. (2006) Molekularzytogenetische Analyse menschlicher Polkörper zur Präimplantationsdiagnostik, Mathematisch-Naturwissenschaftliche Fakultät. Christian-Albrechts-Universität Kiel

13.

Schmutzler A, Glander M, Acar B, Weimer J, Hedderich J, Salmassi A, Arnold N, Mettler L (2009) Genetic imbalances of human oocytes according to zona thickness and size of the perivitelline space—preliminary data. US Obstetric Gynaecol 4:39–43

14.

Bertrand E, Van den Bergh M, Englert Y (1995) Does zona pellucida thickness influence the fertilization rate? Hum Reprod 10:1189–1193PubMed

15.

Garside WT, Loret de Mola JR, Bucci JA, Tureck RW, Heyner S (1997) Sequential analysis of zona thickness during in vitro culture of human zygotes: correlation with embryo quality, age, and implantation. Mol Reprod Dev 47:99–104PubMedCrossRef

16.

Gabrielsen A, Bhatnager PR, Petersen K, Lindenberg S (2000) Influence of zona pellucida thickness of human embryos on clinical pregnancy outcome following in vitro fertilization treatment. J Assist Reprod Genet 17:323–328PubMedCrossRef

17.

Gabrielsen A, Lindenberg S, Petersen K (2001) The impact of the zona pellucida thickness variation of human embryos on pregnancy outcome in relation to suboptimal embryo development. A prospective randomized controlled study. Hum Reprod 16:2166–2170PubMedCrossRef

18.

Host E, Gabrielsen A, Lindenberg S, Smidt-Jensen S (2002) Apoptosis in human cumulus cells in relation to zona pellucida thickness variation, maturation stage, and cleavage of the corresponding oocyte after intracytoplasmic sperm injection. Fertil Steril 77:511–515PubMedCrossRef

19.

Sun YP, Xu Y, Cao T, Su YC, Guo YH (2005) Zona pellucida thickness and clinical pregnancy outcome following in vitro fertilization. Int J Gynaecol Obstet 89:258–262PubMedCrossRef

20.

Shen Y, Stalf T, Mehnert C, Eichenlaub-Ritter U, Tinneberg HR (2005) High magnitude of light retardation by the zona pellucida is associated with conception cycles. Hum Reprod 20:1596–1606PubMedCrossRef

21.

Landwehr C, Montag M, van der Ven K, Weber RG (2008) Rapid comparative genomic hybridization protocol for prenatal diagnosis and its application to aneuploidy screening of human polar bodies. Fertil Steril 90:488–496PubMedCrossRef

22.

De Sutter P, Dozortsev D, Qian C, Dhont M (1996) Oocyte morphology does not correlate with fertilization rate and embryo quality after intracytoplasmic sperm injection. Hum Reprod 11:595–597PubMedCrossRef

23.

Balaban B, Urman B, Sertac A, Alatas C, Aksoy S, Mercan R (1998) Oocyte morphology does not affect fertilization rate, embryo quality and implantation rate after intracytoplasmic sperm injection. Hum Reprod 13:3431–3433PubMedCrossRef

24.

Xia P (1997) Intracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality. Hum Reprod 12:1750–1755PubMedCrossRef

25.

Ebner T, Moser M, Yaman C, Feichtinger O, Hartl J, Tews G (1999) Elective transfer of embryos selected on the basis of first polar body morphology is associated with increased rates of implantation and pregnancy. Fertil Steril 72:599–603PubMedCrossRef

26.

Ebner T, Yaman C, Moser M, Sommergruber M, Feichtinger O, Tews G (2000) Prognostic value of first polar body morphology on fertilization rate and embryo quality in intracytoplasmic sperm injection. Hum Reprod 15:427–430PubMedCrossRef

27.

Ebner T, Moser M, Sommergruber M, Yaman C, Pfleger U, Tews G (2002) First polar body morphology and blastocyst formation rate in ICSI patients. Hum Reprod 17:2415–2418PubMedCrossRef

28.

Younis JS, Radin O, Mirsky N, Izhaki I, Majara T, Bar-ami S, Ben-ami M (2008) First polar body and nucleolar precursor body morphology is related to the ovarian reserve of infertile women. Reprod Biomed Online 16:851–858PubMedCrossRef

29.

Rienzi L, Ubaldi FM, Iacobelli M, Minasi MG, Romano S, Ferrero S, Sapienza F, Baroni E, Litwicka K, Greco E (2008) Significance of metaphase II human oocyte morphology on ICSI outcome. Fertil Steril 90:1692–1700PubMedCrossRef

30.

Verlinsky Y, Lerner S, Illkevitch N, Kuznetsov V, Kuznetsov I, Cieslak J, Kuliev A (2003) Is there any predictive value of first polar body morphology for embryo genotype or developmental potential? Reprod Biomed Online 7:336–341PubMedCrossRef

31.

Ciotti PM, Notarangelo L, Morselli-Labate AM, Felletti V, Porcu E, Venturoli S (2004) First polar body morphology before ICSI is not related to embryo quality or pregnancy rate. Hum Reprod 19:2334–2339PubMedCrossRef

32.

De Santis L, Cino I, Rabellotti E, Calzi F, Persico P, Borini A, Coticchio G (2005) Polar body morphology and spindle imaging as predictors of oocyte quality. Reprod Biomed Online 11:36–42PubMedCrossRef

33.

Farhi J, Nahum H, Weissman A, Zahalka N, Glezerman M, Levran D (2002) Coarse granulation in the perivitelline space and IVF-ICSI outcome. J Assist Reprod Genet 19:545–549PubMedCrossRef

34.

Hassan-Ali H, Hisham-Saleh A, El-Gezeiry D, Baghdady I, Ismaeil I, Mandelbaum J (1998) Perivitelline space granularity: a sign of human menopausal gonadotrophin overdose in intracytoplasmic sperm injection. Hum Reprod 13:3425–3430PubMedCrossRef

35.

Serhal PF, Ranieri DM, Kinis A, Marchant S, Davies M, Khadum IM (1997) Oocyte morphology predicts outcome of intracytoplasmic sperm injection. Hum Reprod 12:1267–1270PubMedCrossRef

36.

Loutradis D, Drakakis P, Kallianidis K, Milingos S, Dendrinos S, Michalas S (1999) Oocyte morphology correlates with embryo quality and pregnancy rate after intracytoplasmic sperm injection. Fertil Steril 72:240–244PubMedCrossRef

37.

Ebner T, Moser M, Sommergruber M, Gaiswinkler U, Shebl O, Jesacher K, Tews G (2005) Occurrence and developmental consequences of vacuoles throughout preimplantation development. Fertil Steril 83:1635–1640PubMedCrossRef

38.

Magli MC, Montag M, Koster M, Muzi L, Geraedts J, Collins J, Goossens V, Handyside AH, Harper J, Repping S, Schmutzler A, Vesela K, Gianaroli L (2011) Polar body array CGH for prediction of the status of the corresponding oocyte. Part II: technical aspects. Hum Reprod 26:3181–3185PubMedCrossRef

39.

Kahraman S, Yakin K, Donmez E, Samli H, Bahce M, Cengiz G, Sertyel S, Samli M, Imirzalioglu N (2000) Relationship between granular cytoplasm of oocytes and pregnancy outcome following intracytoplasmic sperm injection. Hum Reprod 15:2390–2393PubMedCrossRef

40.

Tesarik J, Junca AM, Hazout A, Aubriot FX, Nathan C, Cohen-Bacrie P, Dumont-Hassan M (2000) Embryos with high implantation potential after intracytoplasmic sperm injection can be recognized by a simple, non-invasive examination of pronuclear morphology. Hum Reprod 15:1396–1399PubMedCrossRef

41.

Scott L, Alvero R, Leondires M, Miller B (2000) The morphology of human pronuclear embryos is positively related to blastocyst development and implantation. Hum Reprod 15:2394–2403PubMedCrossRef

Titel: Oocyte morphology on day 0 correlates with aneuploidy as detected by polar body biopsy and FISH
verfasst von: Andreas G. Schmutzler
Bengi Acar-Perk
Jörg Weimer
Ali Salmassi
Knud Sievers
Monica Tobler
Liselotte Mettler
Walter Jonat
Norbert Arnold
Publikationsdatum: 01.02.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 2/2014
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-013-2944-3

Neu im Fachgebiet Gynäkologie und Geburtshilfe

23.04.2024 | Medikamente in Schwangerschaft und Stillzeit | Nachrichten

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Springer Medizin

Oocyte morphology on day 0 correlates with aneuploidy as detected by polar body biopsy and FISH

Abstract

Purpose

Method

Result

Conclusion

Neu im Fachgebiet Gynäkologie und Geburtshilfe

ICI-Therapie in der Schwangerschaft wird gut toleriert

Weniger postpartale Depressionen nach Esketamin-Einmalgabe

Bei RSV-Impfung vor 60. Lebensjahr über Off-Label-Gebrauch aufklären!

Harnwegsinfektprophylaxe: Es geht auch ohne Antibiotika

Update Gynäkologie

Springer Medizin

Abstract

Purpose

Method

Result

Conclusion

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2014

Log-linear human chorionic gonadotropin elimination in cases of retained placenta percreta

The necessity of using tenaculum for endometrial sampling procedure with pipelle: a randomized controlled study

Four countries, four ways of discussing low-risk pregnancy and normal delivery: in France, Germany, The Netherlands, and the United Kingdom

Influence of fetal birth weight on perinatal outcome in planned vaginal births

Clinicopathological and molecular markers associated with prognosis and treatment effectiveness of endometrial stromal sarcoma: a retrospective study in China

Female patients with pseudomyxoma peritonei: a single-institution clinicopathologic study of 35 cases

Neu im Fachgebiet Gynäkologie und Geburtshilfe

ICI-Therapie in der Schwangerschaft wird gut toleriert

Weniger postpartale Depressionen nach Esketamin-Einmalgabe

Bei RSV-Impfung vor 60. Lebensjahr über Off-Label-Gebrauch aufklären!

Harnwegsinfektprophylaxe: Es geht auch ohne Antibiotika

Update Gynäkologie