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Archives of Gynecology and Obstetrics

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24.09.2018 | Gynecologic Endocrinology and Reproductive Medicine

Effects of superovulation, in vitro fertilization, and oocyte in vitro maturation on imprinted gene Grb10 in mouse blastocysts

verfasst von: Xuan Chen, Yanfang Huang, Hongfeng Huang, Yingying Guan, Ming Li, Xiaohong Jiang, Miao Yu, Xiaoyu Yang

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 6/2018

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Abstract

Purpose

Grb10 is a key imprinted gene that is suspected to have a role in the adverse outcomes of assisted reproductive technology (ART), but little is known about the effects of ART on it. Primary ART techniques, including superovulation, in vitro fertilization (IVF), and oocyte in vitro maturation (IVM), were analyzed in this study of the effects of ART on embryo quality and Grb10.

Methods

Embryo development rates were determined. Blastocyst cell number and global methylation were analyzed at the single-embryo level, together with Grb10 methylation and mRNA expression of the imprinted genes.

Results

Lower blastocyst cell number, higher genome and Grb10 CGI1 methylation, and variable mRNA expression were observed in the ART groups compared with the control group. Whether fertilization was in vivo or in vitro, the changes in the genome and Grb10 CGI1 methylation level and Grb10 and H19 expression were similar in the groups with superovulation and more significant than the IVM group.

Conclusions

These results suggest that superovulation had a greater impact than IVF or IVM on the genome and Grb10 DNA methylation level, and Grb10 and H19 expression.

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Titel: Effects of superovulation, in vitro fertilization, and oocyte in vitro maturation on imprinted gene Grb10 in mouse blastocysts
verfasst von: Xuan Chen
Yanfang Huang
Hongfeng Huang
Yingying Guan
Ming Li
Xiaohong Jiang
Miao Yu
Xiaoyu Yang
Publikationsdatum: 24.09.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 6/2018
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-018-4905-3

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Effects of superovulation, in vitro fertilization, and oocyte in vitro maturation on imprinted gene Grb10 in mouse blastocysts

Abstract

Purpose

Methods

Results

Conclusions

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Purpose

Methods

Results

Conclusions

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